@prefix dbo: .
@prefix dbr: .
dbr:Luciferase dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Citrullination dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Restriction_site_associated_DNA_markers dbo:wikiPageWikiLink dbr:Biotinylation .
@prefix foaf: .
@prefix wikipedia-en: .
wikipedia-en:Biotinylation foaf:primaryTopic dbr:Biotinylation .
dbr:Time-resolved_RNA_sequencing dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Stress_granule dbo:wikiPageWikiLink dbr:Biotinylation .
dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Dideoxynucleotide dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Biotin dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:BLESS dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Serial_analysis_of_gene_expression dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:ELISpot dbo:wikiPageWikiLink dbr:Biotinylation .
dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Janus_particles dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Bioconjugation dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Holocarboxylase_synthetase dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Meir_Wilchek dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Protein_tag dbo:wikiPageWikiLink dbr:Biotinylation .
dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Post-translational_modification dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Chemoproteomics dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Chem-seq dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:NeutrAvidin dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Biotin_label dbo:wikiPageWikiLink dbr:Biotinylation ;
dbo:wikiPageRedirects dbr:Biotinylation .
dbr:Biotin_labeling dbo:wikiPageWikiLink dbr:Biotinylation ;
dbo:wikiPageRedirects dbr:Biotinylation .
dbr:Biotin_marker dbo:wikiPageWikiLink dbr:Biotinylation ;
dbo:wikiPageRedirects dbr:Biotinylation .
dbr:Biotinilation dbo:wikiPageWikiLink dbr:Biotinylation ;
dbo:wikiPageRedirects dbr:Biotinylation .
dbr:P-bodies dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Electrophoretic_mobility_shift_assay dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Epitranscriptome dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Proximity_labeling dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:MHC_multimer dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Avidin dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Streptavidin dbo:wikiPageWikiLink dbr:Biotinylation .
@prefix rdf: .
@prefix yago: .
dbr:Biotinylation rdf:type yago:OrganicProcess113526110 ,
dbo:Election ,
yago:Process100029677 ,
yago:WikicatBiologicalProcesses ,
yago:PhysicalEntity100001930 .
@prefix rdfs: .
dbr:Biotinylation rdfs:label "Biotinylation"@en ,
"\u751F\u7269\u7D20\u5316"@zh ,
"Biotinylierung"@de ;
rdfs:comment "Die Biotinylierung bezeichnet den Vorgang der kovalenten Bindung von Biotin an ein Molek\u00FCl, meistens ein Protein oder DNA. Dies geschieht bei manchen Proteinen als posttranslationale Modifikation. Die Biotinylierung wird auch im Labor gezielt eingesetzt, um ein Molek\u00FCl zu Zwecken einer Molek\u00FClmarkierung (z. B. bei einer Detektion im ELISA, ELISPOT, Immunhistochemie und Western Blot) oder einer Aufreinigung zu markieren. Anschlie\u00DFend macht man sich die starke spezifische Wechselwirkung zwischen Biotin und Avidin bzw. Streptavidin zunutze."@de ,
"In biochemistry, biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule. Biotinylation is rapid, specific and is unlikely to disturb the natural function of the molecule due to the small size of biotin (MW = 244.31 g/mol). Biotin binds to streptavidin and avidin with an extremely high affinity, fast on-rate, and high specificity, and these interactions are exploited in many areas of biotechnology to isolate biotinylated molecules of interest. Biotin-binding to streptavidin and avidin is resistant to extremes of heat, pH and proteolysis, making capture of biotinylated molecules possible in a wide variety of environments. Also, multiple biotin molecules can be conjugated to a protein of interest, which allows binding of multiple streptavidin,"@en ,
"\u5728\u751F\u7269\u5316\u5B66\u4E2D\uFF0C\u751F\u7269\u7D20\u5316\uFF08\u82F1\u8A9E\uFF1Abiotinylation\uFF09\u662F\u6307\u5C06\u751F\u7269\u7D20\u5171\u4EF7\u8FDE\u63A5\u5230\u86CB\u767D\u8D28\u3001\u6838\u9178\u6216\u5176\u5B83\u5206\u5B50\u4E0A\u7684\u8FC7\u7A0B\u3002\u7531\u4E8E\u751F\u7269\u7D20\u7684\u5206\u5B50\u91CF\u4E0D\u5927\uFF08\u5206\u5B50\u91CF\u4E3A244.31\uFF09\uFF0C\u751F\u7269\u7D20\u5316\u53CD\u5E94\u5FEB\u901F\u3001\u9AD8\u6548\u4E14\u4E0D\u6613\u88AB\u5E72\u6270\u3002\u751F\u7269\u7D20\u5316\u7684\u5206\u5B50\u80FD\u901A\u8FC7\u751F\u7269\u7D20\u4E0E\u94FE\u9709\u4EB2\u548C\u7D20\u3001\u4E92\u4F5C\uFF0C\u4E14\u4E0D\u53D7\u9AD8\u70ED\u3001pH\u3001\u86CB\u767D\u9176\u89E3\u7684\u5F71\u54CD\u3002\u751F\u7269\u7D20\u4E0E\u94FE\u9709\u4EB2\u548C\u7D20\u3001\u4EB2\u548C\u7D20\u7684\u7ED3\u5408\u7279\u5F02\u4E14\u9AD8\u6548\uFF0C\u56E0\u6B64\u8FD9\u4E00\u4E92\u4F5C\u5728\u751F\u7269\u6280\u672F\u7684\u8BB8\u591A\u9886\u57DF\u6709\u7740\u5E7F\u6CDB\u7684\u5E94\u7528\u3002\u53E6\u5916\uFF0C\u591A\u4E2A\u751F\u7269\u7D20\u5316\u5206\u5B50\u53EF\u4EE5\u53D1\u751F\u4EA4\u8054\u6765\u5F62\u6210\u4E2A\u79D1\u7814\u4EBA\u5458\u611F\u5174\u8DA3\u7684\u86CB\u767D\uFF0C\u540C\u65F6\u5141\u8BB8\u548C\u591A\u4E2A\u94FE\u9709\u4EB2\u548C\u7D20\u3001\u4EB2\u548C\u7D20\u3001\u86CB\u767D\u7ED3\u5408\uFF0C\u589E\u52A0\u4E86\u5BF9\u6B64\u86CB\u767D\u7684\u68C0\u6D4B\u7075\u654F\u5EA6\u3002\u6B64\u5916\u8FD8\u6709\u5927\u91CF\u7684\u751F\u7269\u7D20\u5316\u5206\u5B50\u7684\u5E94\u7528\u6709\u5F85\u5F00\u53D1\u3002"@zh ;
foaf:depiction ,
.
@prefix dct: .
@prefix dbc: .
dbr:Biotinylation dct:subject dbc:Biological_processes ;
dbo:abstract "In biochemistry, biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule. Biotinylation is rapid, specific and is unlikely to disturb the natural function of the molecule due to the small size of biotin (MW = 244.31 g/mol). Biotin binds to streptavidin and avidin with an extremely high affinity, fast on-rate, and high specificity, and these interactions are exploited in many areas of biotechnology to isolate biotinylated molecules of interest. Biotin-binding to streptavidin and avidin is resistant to extremes of heat, pH and proteolysis, making capture of biotinylated molecules possible in a wide variety of environments. Also, multiple biotin molecules can be conjugated to a protein of interest, which allows binding of multiple streptavidin, avidin or neutravidin protein molecules and increases the sensitivity of detection of the protein of interest. There is a large number of biotinylation reagents available that exploit the wide range of possible labelling methods. Due to the strong affinity between biotin and streptavidin, the purification of biotinylated proteins has been a widely used approach to identify protein-protein interactions and post-translational events such as ubiquitylation in molecular biology."@en ,
"Die Biotinylierung bezeichnet den Vorgang der kovalenten Bindung von Biotin an ein Molek\u00FCl, meistens ein Protein oder DNA. Dies geschieht bei manchen Proteinen als posttranslationale Modifikation. Die Biotinylierung wird auch im Labor gezielt eingesetzt, um ein Molek\u00FCl zu Zwecken einer Molek\u00FClmarkierung (z. B. bei einer Detektion im ELISA, ELISPOT, Immunhistochemie und Western Blot) oder einer Aufreinigung zu markieren. Anschlie\u00DFend macht man sich die starke spezifische Wechselwirkung zwischen Biotin und Avidin bzw. Streptavidin zunutze."@de ,
"\u5728\u751F\u7269\u5316\u5B66\u4E2D\uFF0C\u751F\u7269\u7D20\u5316\uFF08\u82F1\u8A9E\uFF1Abiotinylation\uFF09\u662F\u6307\u5C06\u751F\u7269\u7D20\u5171\u4EF7\u8FDE\u63A5\u5230\u86CB\u767D\u8D28\u3001\u6838\u9178\u6216\u5176\u5B83\u5206\u5B50\u4E0A\u7684\u8FC7\u7A0B\u3002\u7531\u4E8E\u751F\u7269\u7D20\u7684\u5206\u5B50\u91CF\u4E0D\u5927\uFF08\u5206\u5B50\u91CF\u4E3A244.31\uFF09\uFF0C\u751F\u7269\u7D20\u5316\u53CD\u5E94\u5FEB\u901F\u3001\u9AD8\u6548\u4E14\u4E0D\u6613\u88AB\u5E72\u6270\u3002\u751F\u7269\u7D20\u5316\u7684\u5206\u5B50\u80FD\u901A\u8FC7\u751F\u7269\u7D20\u4E0E\u94FE\u9709\u4EB2\u548C\u7D20\u3001\u4E92\u4F5C\uFF0C\u4E14\u4E0D\u53D7\u9AD8\u70ED\u3001pH\u3001\u86CB\u767D\u9176\u89E3\u7684\u5F71\u54CD\u3002\u751F\u7269\u7D20\u4E0E\u94FE\u9709\u4EB2\u548C\u7D20\u3001\u4EB2\u548C\u7D20\u7684\u7ED3\u5408\u7279\u5F02\u4E14\u9AD8\u6548\uFF0C\u56E0\u6B64\u8FD9\u4E00\u4E92\u4F5C\u5728\u751F\u7269\u6280\u672F\u7684\u8BB8\u591A\u9886\u57DF\u6709\u7740\u5E7F\u6CDB\u7684\u5E94\u7528\u3002\u53E6\u5916\uFF0C\u591A\u4E2A\u751F\u7269\u7D20\u5316\u5206\u5B50\u53EF\u4EE5\u53D1\u751F\u4EA4\u8054\u6765\u5F62\u6210\u4E2A\u79D1\u7814\u4EBA\u5458\u611F\u5174\u8DA3\u7684\u86CB\u767D\uFF0C\u540C\u65F6\u5141\u8BB8\u548C\u591A\u4E2A\u94FE\u9709\u4EB2\u548C\u7D20\u3001\u4EB2\u548C\u7D20\u3001\u86CB\u767D\u7ED3\u5408\uFF0C\u589E\u52A0\u4E86\u5BF9\u6B64\u86CB\u767D\u7684\u68C0\u6D4B\u7075\u654F\u5EA6\u3002\u6B64\u5916\u8FD8\u6709\u5927\u91CF\u7684\u751F\u7269\u7D20\u5316\u5206\u5B50\u7684\u5E94\u7528\u6709\u5F85\u5F00\u53D1\u3002"@zh ;
dbo:wikiPageWikiLink dbr:Solubility ,
dbr:Biochemistry ,
dbr:Sialic_acids ,
dbr:Proximity_labeling ,
dbr:UV_light ,
dbr:Citrate ,
dbr:Chemical_reaction ,
dbr:ELISPOT ,
dbr:Aldehydes ,
dbr:Half-life ,
dbr:ELISA ,
dbr:Alkaline_phosphatase ,
dbr:Western_blot ,
dbr:Biotin_holoenzyme_synthetase ,
dbr:Carbohydrate ,
dbr:Iminobiotin ,
dbr:Glycine ,
dbr:Epsilon-amine ,
,
dbr:N-hydroxysuccinimide ,
dbr:Non-covalent_bond ,
dbr:Carboxylic_acid ,
,
dbr:Oligonucleotide ,
dbr:Covalent_bond ,
dbr:Thiol ,
dbr:Nucleotides ,
,
,
dbr:Aqueous_solution ,
dbr:Peptides ,
dbr:Red_blood_cells ,
,
dbr:Major_histocompatibility_complex ,
dbr:Acetate ,
dbr:Cysteine ,
dbr:Ubiquitylation ,
dbr:Molecules ,
dbr:Sulfonate ,
dbr:Sodium_periodate ,
dbr:Organic_compound ,
dbr:Carbodiimide ,
dbr:Antibody ,
dbr:Fluorophore ,
dbr:Cross-link ,
dbr:Coomassie_brilliant_blue ,
dbr:Glycoproteins ,
,
dbr:Maleimide ,
dbr:Polyethylene_glycol ,
dbr:Cell_membrane ,
dbr:Avidin ,
dbr:Protein ,
dbr:Dimethyl_sulfoxide ,
dbr:Biotin ,
dbr:Hydrolysis ,
dbr:Neutravidin ,
dbr:Streptavidin ,
dbr:PH ,
dbr:Agarose_gel_electrophoresis ,
dbr:Affinity_chromatography ,
,
dbr:Biotin_carboxyl_carrier_protein ,
dbr:Polyacrylamide_gel_electrophoresis ,
dbc:Biological_processes ,
dbr:Polyclonal_antibodies ,
dbr:Solvent ,
dbr:Oligonucleotide_synthesis ,
dbr:MHC_multimer ,
dbr:Tris ,
dbr:Ultraviolet_light ,
dbr:T-cell ,
dbr:Horseradish_peroxidase ,
dbr:Hydrazine ,
dbr:Dimethyl_formamide .
@prefix dbp: .
@prefix dbt: .
dbr:Biotinylation dbp:wikiPageUsesTemplate dbt:Reflist ,
dbt:Refimprove ,
dbt:ISBN ,
dbt:UniProt ,
dbt:Cite_journal ,
dbt:Protein_sequence ;
dbo:thumbnail ;
dbo:wikiPageRevisionID 1051528521 .
@prefix ns11: .
dbr:Biotinylation dbo:wikiPageExternalLink ns11:biotinylation .
@prefix xsd: .
dbr:Biotinylation dbo:wikiPageLength "23098"^^xsd:nonNegativeInteger ;
dbo:wikiPageID 909363 .
@prefix owl: .
@prefix dbpedia-de: .
dbr:Biotinylation owl:sameAs dbpedia-de:Biotinylierung ,
,
.
@prefix yago-res: .
dbr:Biotinylation owl:sameAs yago-res:Biotinylation .
@prefix dbpedia-sl: .
dbr:Biotinylation owl:sameAs dbpedia-sl:Biotinilacija_histonov .
@prefix wikidata: .
dbr:Biotinylation owl:sameAs wikidata:Q864854 ,
dbr:Biotinylation ,
.
@prefix gold: .
dbr:Biotinylation gold:hypernym dbr:Process .
@prefix prov: .
dbr:Biotinylation prov:wasDerivedFrom ;
foaf:isPrimaryTopicOf wikipedia-en:Biotinylation .
dbr:Immunostaining dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Cell-free_protein_array dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Photobiotin dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Molecular_Inversion_Probe dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Nanopore_sequencing dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Paleogenomics dbo:wikiPageWikiLink dbr:Biotinylation .
dbr:Eastern_blot dbo:wikiPageWikiLink dbr:Biotinylation .