Caenorhabditis Intervention Testing Program: Healthspan Protocols

The Caenorhabditis Intervention Testing Program (CITP) is a multi-institutional program that seeks robust and reproducible pro-longevity interventions that are efficacious across genetically diverse cohorts in the Caenorhabditis genus. The project features a unique design in which multiple experimental replicates are collected by three different laboratories at discrete locations for all candidate compounds tested. To limit confounding factors that may influence experimental reproducibility, we develop standardized operating procedures (SOPs) for all experimental procedures. Here we provide SOPs for the health assays used by the CITP.