Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Brief Communication
  • Published:

Adult-born SVZ progenitors receive transient synapses during remyelination in corpus callosum

Nature Neuroscience volume 13, pages 287–289 (2010)Cite this article

Subjects

Abstract

We found that demyelinated axons formed functional glutamatergic synapses onto adult-born NG2+ oligodendrocyte progenitor cells (OPCs) migrating from the subventricular zone after focal demyelination of adult mice corpus callosum. This glutamatergic input was substantially reduced compared with endogenous callosal OPCs 1 week after lesion and was lost on differentiation into oligodendrocytes. Therefore, axon–oligodendrocyte progenitor synapse formation is a transient and regulated step that occurs during remyelination of callosal axons.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Adult-born SVZ NG2-positive cells display synaptic currents after migrating into a demyelinated lesion of the corpus callosum.
Figure 2: Synaptically connected SVZ-derived OPCs give rise to oligodendrocytes.
Figure 3: Glutamatergic synaptic transmission between axons and NG2-positive cells is reduced 1 week after demyelination.

Similar content being viewed by others

References

  1. Gensert, J.M. & Goldman, J.E. Neuron 19, 197–203 (1997).

    Article  CAS  PubMed  Google Scholar 

  2. Nait-Oumesmar, B. et al. Eur. J. Neurosci. 11, 4357–4366 (1999).

    Article  CAS  PubMed  Google Scholar 

  3. Menn, B. et al. J. Neurosci. 26, 7907–7918 (2006).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Aguirre, A., Dupree, J., Mangin, J.M. & Gallo, V. Nat. Neurosci. 10, 990–1002 (2007).

    Article  CAS  PubMed  Google Scholar 

  5. Kukley, M., Capetillo-Zarate, E. & Dietrich, D. Nat. Neurosci. 10, 311–320 (2007).

    Article  CAS  PubMed  Google Scholar 

  6. Ziskin, J.L., Nishiyama, A., Rubio, M., Fukaya, M. & Bergles, D.E. Nat. Neurosci. 10, 321–330 (2007).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Chittajallu, R., Aguirre, A.A. & Gallo, V. J. Neurosci. 25, 8601–8610 (2005).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Bezzi, P. et al. Nat. Neurosci. 7, 613–620 (2004).

    Article  CAS  PubMed  Google Scholar 

  9. Gallo, V. et al. J. Neurosci. 16, 2659–2670 (1996).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Yuan, X., Eisen, A.M., McBain, C.J. & Gallo, V. Development 125, 2901–2914 (1998).

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank F. Gage (Salk Institute) and J. Goldman (Columbia University) for the pNIT-GFP retrovirus, A. Nishiyama (University of Connecticut) for the NG2-dsRed mouse and D. Rowitch (University of California San Francisco) for antibody to Olig2. We are particularly grateful to J. Corbin for discussion and to R. McCarter for help with statistical analysis. This work was supported by grants from the US National Institutes of Health (R01NS045702 and R01NS045702 to V.G., K99NS057944 to A.A. and Intellectual and Developmental Disabilities Research Center P30HD40677). A.E. was supported by a Postdoctoral Fellowship from the Department of Education and Science of the Spanish Government.

Author information

Author notes

  1. Ainhoa Etxeberria and Jean-Marie Mangin: These authors contributed equally to this work.

Authors and Affiliations

  1. Center for Neuroscience Research, Children's National Medical Center, Washington, DC, USA

    Ainhoa Etxeberria, Jean-Marie Mangin, Adan Aguirre & Vittorio Gallo

Authors
  1. Ainhoa Etxeberria
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jean-Marie Mangin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Adan Aguirre
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Vittorio Gallo
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

A.E. and J.-M.M. designed, performed and analyzed all of the experiments. A.A. helped with the LPC injection experiments and with the preparation of the retrovirus. V.G. designed the experiments with A.E. and J.-M.M. supervised the project and wrote the manuscript with A.E. and J.-M.M.

Corresponding author

Correspondence to Vittorio Gallo.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–6 and Supplementary Methods (PDF 2713 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Etxeberria, A., Mangin, JM., Aguirre, A. et al. Adult-born SVZ progenitors receive transient synapses during remyelination in corpus callosum. Nat Neurosci 13, 287–289 (2010). https://doi.org/10.1038/nn.2500

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nn.2500

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing
  NODES
INTERN 1
Note 1
Project 1
twitter 1