CRISPRz
CRISPR/Cas9 has emerged as a versatile genome editing tool and is being used in a wide variety of cell lines and organisms including zebrafish. The Burgess lab has developed a streamlined pipeline for generating gene knockouts using CRISPR/Cas9 in a high-throughput manner.
CRISPRz is a curated database of validated CRISPR _targets in zebrafish maintained by Shawn Burgess' lab at NHGRI/NIH. The database contains _target sites from the Burgess lab's large-scale mutagenesis project, as well as those reported in the literature. A search form allows users to search by various identifiers in order to determine if a validated CRISPR _target exists for a gene of interest.
The CRISPRz site also hosts methods and protocols developed by the Burgess lab, software for identifying indels generated by CRISPR/Cas9 (ampliconDIVider), and links to CRISPR _target tracks on the UCSC Genome browser.
We welcome submissions to CRISPRz. If you wish to add CRISPR _target activity data from your lab, please use fill out this template and email to: Gaurav Varshney at Gaurav-varshney@omrf.org
To get CRISPR _targets tracks at the UCSC Genome Browser, please click HERE.
Sept 24, 2018 data release (genome assembly danRer11):
Current CRISPR _target sequences: 1759. Number of Sources: 14
To get CRISPR _targets in genome assembly ZV9, please click HERE
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Last modified:
Friday October 09, 2020
