Nobiletin, a citrus flavonoid, suppresses phorbol ester-induced expression of multiple scavenger receptor genes in THP-1 human monocytic cells
- PMID: 16698017
- DOI: 10.1016/j.febslet.2006.04.077
Nobiletin, a citrus flavonoid, suppresses phorbol ester-induced expression of multiple scavenger receptor genes in THP-1 human monocytic cells
Abstract
Unregulated uptake of oxidized low-density lipoproteins (ox-LDL) via macrophage scavenger receptors (SRs) such as lectin-like ox-LDL receptor-1 (LOX-1) is a key event in atherosclerosis. In this study, we examined the effects of five selected food phytochemicals on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced LOX-1 mRNA expression in THP-1 human monocyte-like cells. Nobiletin, a citrus polymethoxylated flavone, markedly reduced it in dose- and time-dependent manners. It also suppressed the phosphorylation of extracellular signal-regulated protein kinase (ERK) 1/2, c-Jun NH2-terminal kinase (JNK) 1/2, and c-Jun (Ser-63), thereby inhibiting the transcriptional activity of activator protein-1. Further nobiletin attenuated expression of SR-A, SR-PSOX, CD36, and CD68, but not CLA-1, mRNA, leading to the blockade of DiI-acLDL uptake. Together, our results suggest that nobiletin is a promising phytochemical for regulating atherosclerosis with reasonable action mechanisms.
Similar articles
-
Zerumbone suppresses phorbol ester-induced expression of multiple scavenger receptor genes in THP-1 human monocytic cells.Biosci Biotechnol Biochem. 2007 Apr;71(4):935-45. doi: 10.1271/bbb.60596. Epub 2007 Apr 7. Biosci Biotechnol Biochem. 2007. PMID: 17420607
-
Suppressive effects of demethylated metabolites of nobiletin on phorbol ester-induced expression of scavenger receptor genes in THP-1 human monocytic cells.Biofactors. 2007;31(2):107-16. doi: 10.1002/biof.5520310201. Biofactors. 2007. PMID: 18806314
-
Sp1 is an essential transcription factor for LPS-induced tissue factor expression in THP-1 monocytic cells, and nobiletin represses the expression through inhibition of NF-kappaB, AP-1, and Sp1 activation.Biochem Pharmacol. 2008 Apr 1;75(7):1504-14. doi: 10.1016/j.bcp.2007.12.019. Epub 2008 Jan 5. Biochem Pharmacol. 2008. PMID: 18261712
-
Endothelial scavenger receptors.Prog Lipid Res. 2006 Sep;45(5):379-404. doi: 10.1016/j.plipres.2006.03.002. Epub 2006 Apr 19. Prog Lipid Res. 2006. PMID: 16712941 Review.
-
Effect of the Citrus Flavone Nobiletin on Circadian Rhythms and Metabolic Syndrome.Molecules. 2022 Nov 10;27(22):7727. doi: 10.3390/molecules27227727. Molecules. 2022. PMID: 36431828 Free PMC article. Review.
Cited by
-
Decades Long Involvement of THP-1 Cells as a Model for Macrophage Research: A Comprehensive Review.Antiinflamm Antiallergy Agents Med Chem. 2024;23(2):85-104. doi: 10.2174/0118715230294413240415054610. Antiinflamm Antiallergy Agents Med Chem. 2024. PMID: 38676532 Review.
-
Comparative Activities of Three Compounds from Citrus aurantium L. var. amara Engl. to Improve Oxidized Low-Density Lipoprotein-Induced Lipid Deposition.ACS Omega. 2024 Jan 24;9(5):5683-5694. doi: 10.1021/acsomega.3c08147. eCollection 2024 Feb 6. ACS Omega. 2024. PMID: 38343915 Free PMC article.
-
Citrus flavonoids and adhesion molecules: Potential role in the management of atherosclerosis.Heliyon. 2023 Nov 4;9(11):e21849. doi: 10.1016/j.heliyon.2023.e21849. eCollection 2023 Nov. Heliyon. 2023. PMID: 38028000 Free PMC article. Review.
-
The citrus flavonoid "Nobiletin" impedes STZ-induced Alzheimer's disease in a mouse model through regulating autophagy mastered by SIRT1/FoxO3a mechanism.Inflammopharmacology. 2023 Oct;31(5):2701-2717. doi: 10.1007/s10787-023-01292-z. Epub 2023 Aug 19. Inflammopharmacology. 2023. PMID: 37598127 Free PMC article.
-
Three polymethoxyflavones from the peel of Citrus reticulata "Chachi" inhibits oxidized low-density lipoprotein-induced macrophage-derived foam cell formation.Front Cardiovasc Med. 2022 Jul 28;9:924551. doi: 10.3389/fcvm.2022.924551. eCollection 2022. Front Cardiovasc Med. 2022. PMID: 35966555 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases
Research Materials
Miscellaneous
