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. 2010 Aug 11;132(31):10628-9.
doi: 10.1021/ja101387b.

Rapid visualization and large-scale profiling of bacterial lipoproteins with chemical reporters

Kavita J Rangan  1 , Yu-Ying Yang, Guillaume Charron, Howard C Hang
Affiliations

Rapid visualization and large-scale profiling of bacterial lipoproteins with chemical reporters

Kavita J Rangan et al. J Am Chem Soc. .

Abstract

Lipoproteins are a largely uncharacterized class of proteins in bacteria. In this study, metabolic labeling of bacteria with fatty acid chemical reporters allowed rapid profiling of lipid-modified proteins. We identified many candidate lipoproteins in Escherichia coli and detected a novel modification on YjgF. This chemical approach should facilitate future characterization of lipoproteins.

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Figures

Figure 1
Figure 1
A) Structure of a bacterial lipoprotein. (n = 12 – 20). B) Two-step bioorthogonal labeling strategy using alkynyl-fatty acids.
Figure 2
Figure 2
A) Profiling of E. coli lipoproteins with alk-FA reporters. B) Specific labeling of NlpE-His6 with alk-14. Coomassie blue staining (CB) indicates level of protein loading.
Figure 3
Figure 3
Validation of alk-FA labeled proteins in bacteria. A) GO annotation of high confidence candidates identified from E. coli. Percent LPPs in each category is indicated in parenthesis. B. alk-14 labeling of SlyB and YggG. C) alk-14 labeling of YjgF and YjgFC107S. D) Competition of alk-14 labeling of YjgF with palmitic acid (PA) and sodium acetate (SA).
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