Effects of monoclonal antibodies against human stathmin combined with paclitaxel on proliferation of the QG-56 human lung carcinoma cell line
- PMID: 22938491
- DOI: 10.7314/apjcp.2012.13.6.2967
Effects of monoclonal antibodies against human stathmin combined with paclitaxel on proliferation of the QG-56 human lung carcinoma cell line
Abstract
Objective: To explore whether monoclonal antibodies against stathmin and the chemotherapuetic agent paclitaxel have synergenic effects in inhibiting growth and inducing apoptosis in human QG-56 cells.
Methods: QG-56 cells were treated with monoclonal antibodies against stathmin or paclitaxel alone or in combination, with untreated cells used as controls. After 24, 48, 72 and 96 hours the cell growth condition was observed under an inverted microscope and inhibition was studied by MTT assay; apoptosis was analyzed by flow cytometry.
Results: The populations decreased and cell shape and size changed after the various treatments. Monoclonal antibodies against stathmin and paclitaxel used alone or incombination inhibited the proliferation of QG-56 cells, especially in combination with synergism (P<0.05). Combined treatment also resulted in a significantly higher apoptosis rate than in the other groups (P<0.05).
Conclusions: Monoclonal antibodies against stathmin and paclitaxel used alone or in combination can inhibit proliferation of QG-56 cells and induce apoptosis when applied together, The observed synergistic effects may have important implications for clinical application.
Similar articles
-
Effect of trichostatin A and paclitaxel on the proliferation and apoptosis of lung adenocarcinoma cells.Chin Med J (Engl). 2013 Jan;126(1):129-34. Chin Med J (Engl). 2013. PMID: 23286491
-
A combination of paclitaxel and siRNA-mediated silencing of Stathmin inhibits growth and promotes apoptosis of nasopharyngeal carcinoma cells.Cell Oncol (Dordr). 2014 Feb;37(1):53-67. doi: 10.1007/s13402-013-0163-3. Epub 2013 Dec 5. Cell Oncol (Dordr). 2014. PMID: 24306928
-
[Effect of trichostatin A and paclitaxel on the growth and apoptosis of lung adenocarcinoma cell lines].Zhonghua Zhong Liu Za Zhi. 2012 Jul;34(7):492-6. doi: 10.3760/cma.j.issn.0253-3766.2012.07.004. Zhonghua Zhong Liu Za Zhi. 2012. PMID: 22967465 Chinese.
-
Paclitaxel and vinorelbine cause synergistic increases in apoptosis but not in microtubular disruption in human lung adenocarcinoma cells (A-549).Histochem Cell Biol. 2004 Feb;121(2):115-21. doi: 10.1007/s00418-004-0618-8. Epub 2004 Jan 27. Histochem Cell Biol. 2004. PMID: 14745558
-
Cytotoxicity and apoptosis of ovarian and breast cancer cell lines induced by OVS1 monoclonal antibody and paclitaxel.Asian Pac J Allergy Immunol. 2003 Mar;21(1):31-41. Asian Pac J Allergy Immunol. 2003. PMID: 12931749
Cited by
-
Correlation between microtubule-associated gene expression and chemosensitivity of patients with stage II non-small cell lung cancer.Exp Ther Med. 2013 May;5(5):1506-1510. doi: 10.3892/etm.2013.1007. Epub 2013 Mar 14. Exp Ther Med. 2013. PMID: 23737908 Free PMC article.
-
Stathmin-dependent molecular _targeting therapy for malignant tumor: the latest 5 years' discoveries and developments.J Transl Med. 2016 Sep 27;14(1):279. doi: 10.1186/s12967-016-1000-z. J Transl Med. 2016. PMID: 27670291 Free PMC article. Review.
-
Synergy of Taxol and rhein lysinate associated with the downregulation of ERK activation in lung carcinoma cells.Oncol Lett. 2013 Aug;6(2):525-528. doi: 10.3892/ol.2013.1398. Epub 2013 Jun 14. Oncol Lett. 2013. PMID: 24137360 Free PMC article.
-
High STMN1 level is associated with chemo-resistance and poor prognosis in gastric cancer patients.Br J Cancer. 2017 Apr 25;116(9):1177-1185. doi: 10.1038/bjc.2017.76. Epub 2017 Mar 23. Br J Cancer. 2017. PMID: 28334732 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
