Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Sep;15(3):306-12.
doi: 10.4048/jbc.2012.15.3.306. Epub 2012 Sep 28.

Impact of breast cancer and combination chemotherapy on oxidative stress, hepatic and cardiac markers

Kamal Adel Amin  1 , Basant Mahmoud Mohamed, Mohamed Aly M El-Wakil, Sanaa Omar Ibrahem
Affiliations

Impact of breast cancer and combination chemotherapy on oxidative stress, hepatic and cardiac markers

Kamal Adel Amin et al. J Breast Cancer. 2012 Sep.

Abstract

Purpose: Carcinoma of the breast is the most prevalent cancer among Egyptian women and constitutes 29% of National Cancer Institute cases. The aim of this study was to determine the effect of breast cancer on oxidative stress, cardiac markers and liver function tests, moreover the role of 5-fluorouracil, doxorubicin, and cyclophosphamide (FAC) in the treatment of breast cancer and its mechanism through changing the measured markers.

Methods: Forty female breast cancer patients who were admitted to the Department of Oncology of the Beni-Suef University Hospital were enrolled in the study. This study included three arms: a control group of healthy age-matched females (n=20), breast cancer patients who weren't receiving treatment (n=20), and patients undergoing treatment with anticancer combination drugs FAC (n=20). Blood samples collected from the control subjects and patients were analysed to determine levels of catalase, reduced glutathione (GSH), uric acid, nitric oxide (NO), malondialdehyde, creatine kinase (CK), lactate dehydrogenase (LDH), liver enzymes (alanine aminotransferase and aspartate aminotransferase), and creatinine.

Results: The levels of catalase and GSH were significantly reduced (p<0.05) in breast carcinoma and FAC treated breast cancer patients. The lipid peroxidation and NO levels were significantly enhanced in both untreated and FAC treated breast cancer patients. The CK and LDH were significantly enhanced (p<0.05) in the FAC group.

Conclusion: The results from the present study show that oxidative stress is implicated in breast carcinoma and chemotherapy aggravates this oxidative stress which causes damage to many cellular _targets and has the main side effect of cardiotoxicity.

Keywords: Breast neoplasms; Cardiac function; Chemotherapy; Liver function; Oxidative stress.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
The malondialdehyde (MDA) levels in the control group, breast cancer patients before treatment and chemotherapy treated groups. FAC=5-fluorouracil, doxorubicin, and cyclophosphamide. *Significantly different from the control group; †Significantly different from the breast cancer patients before chemotherapy group.
Figure 2
Figure 2
The nitric oxide concentration in the control group, breast cancer patients before treatment and chemotherapy treated groups. FAC=5-fluorouracil, doxorubicin, and cyclophosphamide. *Significantly different from the control group; †Significantly different from the breast cancer patients before chemotherapy group.
Figure 3
Figure 3
The reduced glutathione (GSH) concentration in the control group, breast cancer patients before treatment and chemotherapy treated groups. FAC=5-fluorouracil, doxorubicin, and cyclophosphamide. *Significantly different from the control group; †Significantly different from the breast cancer patients before chemotherapy group.
Figure 4
Figure 4
The catalase activity in the control group, breast cancer patients before treatment and chemotherapy treated groups. FAC=5-fluorouracil, doxorubicin, and cyclophosphamide. *Significantly different from the control group; †Significantly different from the breast cancer patients before chemotherapy group.
Figure 5
Figure 5
The creatine kinase (CK) activity in the control group, breast cancer patients before treatment and chemotherapy treated groups. FAC=5-fluorouracil, doxorubicin, and cyclophosphamide. *Significantly different from the control group; †Significantly different from the breast cancer patients before chemotherapy group.
Figure 6
Figure 6
The lactate dehydrogenase (LDH) activity in the control group, breast cancer patients before treatment and chemotherapy treated groups. FAC=5-fluorouracil, doxorubicin, and cyclophosphamide. *Significantly different from the control group; †Significantly different from the breast cancer patients before chemotherapy group.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Polyak K. On the birth of breast cancer. Biochim Biophys Acta. 2001;1552:1–13. - PubMed
    1. Gönenç A, Erten D, Aslan S, Akinci M, Simşek B, Torun M. Lipid peroxidation and antioxidant status in blood and tissue of malignant breast tumor and benign breast disease. Cell Biol Int. 2006;30:376–380. - PubMed
    1. Yeh CC, Hou MF, Tsai SM, Lin SK, Hsiao JK, Huang JC, et al. Superoxide anion radical, lipid peroxides and antioxidant status in the blood of patients with breast cancer. Clin Chim Acta. 2005;361:104–111. - PubMed
    1. Kang DH. Oxidative stress, DNA damage, and breast cancer. AACN Clin Issues. 2002;13:540–549. - PubMed
    1. Birnboim HC. DNA strand breakage in human leukocytes exposed to a tumor promoter, phorbol myristate acetate. Science. 1982;215:1247–1249. - PubMed
  NODES
twitter 2