Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Nov 7;13(11):15242-51.
doi: 10.3390/s131115242.

Short chain N-acylhomoserine lactone production by clinical multidrug resistant Klebsiella pneumoniae strain CSG20

Yun Fong Ngeow  1 , Huey Jia Cheng, Jian Woon Chen, Wai-Fong Yin, Kok-Gan Chan
Affiliations

Short chain N-acylhomoserine lactone production by clinical multidrug resistant Klebsiella pneumoniae strain CSG20

Yun Fong Ngeow et al. Sensors (Basel). .

Abstract

Klebsiella pneumoniae is one of the most common Gram-negative bacterial pathogens in clinical practice. It is associated with a wide range of disorders, ranging from superficial skin and soft tissue infections to potentially fatal sepsis in the lungs and blood stream. Quorum sensing, or bacterial cell-cell communication, refers to population density-dependent gene expression modulation. Quorum sensing in Proteobacteria relies on the production and sensing of signaling molecules which are mostly N-acylhomoserine lactones. Here, we report the identification of a multidrug resistant clinical isolate, K. pneumoniae strain CSG20, using matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry. We further confirmed quorum sensing activity in this strain with the use of high resolution tandem liquid chromatography quadrupole mass spectrometry and provided evidence K. pneumoniae strain CSG20 produced N-hexanoyl-homoserine lactone (C6-HSL). To the best of our knowledge, this is the first report on the production of N-hexanoylhomoserine lactone (C6-HSL) in clinical isolate K. pneumoniae.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Score-oriented dendrogram of clinical isolate K. pneumoniae strain CSG2.
Figure 2.
Figure 2.
Diagram of quorum sensing.
Figure 3.
Figure 3.
Mass spectra of AHLs extracted from K. pneumoniae strain CSG20 supernatant. (a) Total ion chromatogram of AHLs extract strain CSG20 and standard C6-HSL. (b) MS analysis of AHLs extract strain CGS20 shown product ion m/z 101.90 and precursor ion m/z 200.1 (Retention time: 1.970 min; Abundance: 439.96; Abundance %: 7.07).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Podschun R., Ullmann U. Klebsiella spp. as nosocomial pathogens: Epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin. Microbiol. Rev. 1998;11:589–603. - PMC - PubMed
    1. Guentzel M.N. Medical Microbiology. 4th ed. Galveston (TX); University of Texas Medical Branch at Galveston, Galveston, TX, USA: 1996. Escherichia, Klebsiella, Enterobacter, Serratia, Citrobacter, and Proteus. - PubMed
    1. Ko W.C., Paterson D.L., Sagnimeni A.J., Hansen D.S., von Gottberg A., Mohapatra S., Casellas J.M., Goossens H., Mulazimoglu L., Trenholme G., et al. Community-acquired Klebsiella pneumoniae bacteremia: Global differences in clinical patterns. Emerg. Infect. Dis. 2002;8:160–166. - PMC - PubMed
    1. McCloud J.M., Jameson J.S. Life-threatening sepsis following treatment for haemorrhoids: A systematic review. Colorectal Dis. 2006;8:748–755. - PubMed
    1. Jong G.M., Hsiue T.R., Chen C.R., Chang H.Y., Chen C.W. Rapidly fatal outcome of bacteremic Klebsiella pneumoniae pneumonia in alcoholics. Chest. J. 1995;107:214–217. - PubMed

Publication types

MeSH terms

LinkOut - more resources

  NODES
COMMUNITY 1
twitter 2