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. 2015 Apr 15;8(4):5954-8.
eCollection 2015.

RhoBTB2 (DBC2) functions as a multifunctional tumor suppressor in thyroid cancer cells via mitochondrial apoptotic pathway

Chuan-Jiang Wang  1 , Dong Yang  1 , Ying-Wei Luo  1
Affiliations

RhoBTB2 (DBC2) functions as a multifunctional tumor suppressor in thyroid cancer cells via mitochondrial apoptotic pathway

Chuan-Jiang Wang et al. Int J Clin Exp Med. .

Abstract

Thyroid cancer is the most common endocrine malignancy worldwide. Tumor suppressor gene RhoBTB2 (also known as Deleted in Breast Cancer 2, DBC2) was observed in various carcinomas, however, no reports showed the effects of RhoBTB2 on thyroid cancer. In our study, we found that RhoBTB2 decreases proliferation, increases apoptosis, inhibits mobility, and induces mitochondria damage in SW579 cells through increased Bax and decreased Bcl-2 and Bcl-xL protein expression. The effects of RhoBTB2 on SW579 cells were inversed by using butin (an inhibitor of the mitochondrial apoptosis pathway). Our results suggest that RhoBTB2 suppresses the growth of SW579 cells through a mitochondrial apoptosis pathway.

Keywords: RhoBTB2; apoptosis; mitochondrial apoptotic pathway; mobility; thyroid cancer.

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Figures

Figure 1
Figure 1
Anti-tumor activities of RhoBTB2 in SW579 cells. A. Proliferation ratio of SW579 cells treated with recombinant RhoBTB2 protein was measured using MTT assay. B. Apoptotic ratio of cells was analyzed by double stained with Annexin-V/propidium iodide (PI). C. Mitochondrial-Membrane Potential was analyzed by Flow cytometry. D. Transwell assays were performed. Cells that migrated to the bottom side of the membrane were stained and counted. E. RhoBTB2 treated SW579 cells showed reduced telomerase activity. These experiments were repeated three times. SW579: untreated SW579 cells; RhoBTB2: RhoBTB2 treated SW579 cells; Butin: both butin and RhoBTB2 treated SW579 cells.
Figure 2
Figure 2
Effects of RhoBTB2 on mitochondrial apoptosis related proteins. Western blot analysis was performed. Cell lysates were electrophoresed and Bax, Bcl-2, Bcl-xL, Bad, Bak proteins were detected by their specific antibodies.
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