Identifying SARS-CoV-2-related coronaviruses in Malayan pangolins

doi:10.1038/s41586-020-2169-0

Comparative Study

. 2020 Jul;583(7815):282-285.

doi: 10.1038/s41586-020-2169-0. Epub 2020 Mar 26.

Identifying SARS-CoV-2-related coronaviruses in Malayan pangolins

Tommy Tsan-Yuk Lam^#^{1

2}, Na Jia^#³, Ya-Wei Zhang^#³, Marcus Ho-Hin Shum^#², Jia-Fu Jiang^#³, Hua-Chen Zhu^{1

2}, Yi-Gang Tong^#⁴, Yong-Xia Shi⁵, Xue-Bing Ni², Yun-Shi Liao², Wen-Juan Li⁴, Bao-Gui Jiang³, Wei Wei⁶, Ting-Ting Yuan³, Kui Zheng⁵, Xiao-Ming Cui³, Jie Li³, Guang-Qian Pei³, Xin Qiang³, William Yiu-Man Cheung², Lian-Feng Li⁷, Fang-Fang Sun⁵, Si Qin³, Ji-Cheng Huang⁵, Gabriel M Leung², Edward C Holmes⁸, Yan-Ling Hu^{9

10}, Yi Guan^{11

12}, Wu-Chun Cao¹³

Affiliations

¹ Joint Institute of Virology (Shantou University and The University of Hong Kong), Guangdong-Hongkong Joint Laboratory of Emerging Infectious Diseases, Shantou University, Shantou, P. R. China.
² State Key Laboratory of Emerging Infectious Diseases, School of Public Health, The University of Hong Kong, Hong Kong, P. R. China.
³ State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, P. R. China.
⁴ Beijing Advanced Innovation Center for Soft Matter Science and Engineering (BAIC-SM), College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, P. R. China.
⁵ Guangzhou Customs Technology Center, Guangzhou, P. R. China.
⁶ Life Sciences Institute, Guangxi Medical University, Nanning, P. R. China.
⁷ School of Information and Management, Guangxi Medical University, Nanning, P. R. China.
⁸ Marie Bashir Institute for Infectious Diseases and Biosecurity, School of Life and Environmental Sciences and School of Medical Sciences, The University of Sydney, Sydney, New South Wales, Australia.
⁹ Life Sciences Institute, Guangxi Medical University, Nanning, P. R. China. huyanling@gxmu.edu.cn.
¹⁰ Center for Genomic and Personalized Medicine, Guangxi Medical University, Nanning, P. R. China. huyanling@gxmu.edu.cn.
¹¹ Joint Institute of Virology (Shantou University and The University of Hong Kong), Guangdong-Hongkong Joint Laboratory of Emerging Infectious Diseases, Shantou University, Shantou, P. R. China. yguan@hku.hk.
¹² State Key Laboratory of Emerging Infectious Diseases, School of Public Health, The University of Hong Kong, Hong Kong, P. R. China. yguan@hku.hk.
¹³ State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, P. R. China. caowc@bmi.ac.cn.

^# Contributed equally.

PMID: 32218527
DOI: 10.1038/s41586-020-2169-0

Comparative Study

Identifying SARS-CoV-2-related coronaviruses in Malayan pangolins

Tommy Tsan-Yuk Lam et al. Nature. 2020 Jul.

. 2020 Jul;583(7815):282-285.

doi: 10.1038/s41586-020-2169-0. Epub 2020 Mar 26.

Authors

Affiliations

¹ Joint Institute of Virology (Shantou University and The University of Hong Kong), Guangdong-Hongkong Joint Laboratory of Emerging Infectious Diseases, Shantou University, Shantou, P. R. China.
² State Key Laboratory of Emerging Infectious Diseases, School of Public Health, The University of Hong Kong, Hong Kong, P. R. China.
³ State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, P. R. China.
⁴ Beijing Advanced Innovation Center for Soft Matter Science and Engineering (BAIC-SM), College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, P. R. China.
⁵ Guangzhou Customs Technology Center, Guangzhou, P. R. China.
⁶ Life Sciences Institute, Guangxi Medical University, Nanning, P. R. China.
⁷ School of Information and Management, Guangxi Medical University, Nanning, P. R. China.
⁸ Marie Bashir Institute for Infectious Diseases and Biosecurity, School of Life and Environmental Sciences and School of Medical Sciences, The University of Sydney, Sydney, New South Wales, Australia.
⁹ Life Sciences Institute, Guangxi Medical University, Nanning, P. R. China. huyanling@gxmu.edu.cn.
¹⁰ Center for Genomic and Personalized Medicine, Guangxi Medical University, Nanning, P. R. China. huyanling@gxmu.edu.cn.
¹¹ Joint Institute of Virology (Shantou University and The University of Hong Kong), Guangdong-Hongkong Joint Laboratory of Emerging Infectious Diseases, Shantou University, Shantou, P. R. China. yguan@hku.hk.
¹² State Key Laboratory of Emerging Infectious Diseases, School of Public Health, The University of Hong Kong, Hong Kong, P. R. China. yguan@hku.hk.
¹³ State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, P. R. China. caowc@bmi.ac.cn.

^# Contributed equally.

PMID: 32218527
DOI: 10.1038/s41586-020-2169-0

Abstract

The ongoing outbreak of viral pneumonia in China and across the world is associated with a new coronavirus, SARS-CoV-2¹. This outbreak has been tentatively associated with a seafood market in Wuhan, China, where the sale of wild animals may be the source of zoonotic infection². Although bats are probable reservoir hosts for SARS-CoV-2, the identity of any intermediate host that may have facilitated transfer to humans is unknown. Here we report the identification of SARS-CoV-2-related coronaviruses in Malayan pangolins (Manis javanica) seized in anti-smuggling operations in southern China. Metagenomic sequencing identified pangolin-associated coronaviruses that belong to two sub-lineages of SARS-CoV-2-related coronaviruses, including one that exhibits strong similarity in the receptor-binding domain to SARS-CoV-2. The discovery of multiple lineages of pangolin coronavirus and their similarity to SARS-CoV-2 suggests that pangolins should be considered as possible hosts in the emergence of new coronaviruses and should be removed from wet markets to prevent zoonotic transmission.

PubMed Disclaimer

Comment in

Pangolins Harbor SARS-CoV-2-Related Coronaviruses.
Han GZ. Han GZ. Trends Microbiol. 2020 Jul;28(7):515-517. doi: 10.1016/j.tim.2020.04.001. Epub 2020 Apr 6. Trends Microbiol. 2020. PMID: 32544437 Free PMC article.

Cited by

Viruses That Can and Cannot Coexist With Humans and the Future of SARS-CoV-2.
Furuse Y, Oshitani H. Furuse Y, et al. Front Microbiol. 2020 Sep 18;11:583252. doi: 10.3389/fmicb.2020.583252. eCollection 2020. Front Microbiol. 2020. PMID: 33042101 Free PMC article.
Latest updates on SARS-CoV-2 genomic characterization, drug, and vaccine development; a comprehensive bioinformatics review.
Sakr MM, Elsayed NS, El-Housseiny GS. Sakr MM, et al. Microb Pathog. 2021 May;154:104809. doi: 10.1016/j.micpath.2021.104809. Epub 2021 Feb 27. Microb Pathog. 2021. PMID: 33647446 Free PMC article. Review.
The comparison of pathogenicity among SARS-CoV-2 variants in domestic cats.
Park ES, Kuroda Y, Uda A, Kaku Y, Okutani A, Hotta A, Tatemoto K, Ishijima K, Inoue Y, Harada M, Ami Y, Shirakura M, Watanabe S, Suzuki Y, Harada T, Ainai A, Shiwa N, Sakai Y, Iwata-Yoshikawa N, Nagata N, Suzuki T, Hasegawa H, Maeda K. Park ES, et al. Sci Rep. 2024 Sep 18;14(1):21815. doi: 10.1038/s41598-024-71791-8. Sci Rep. 2024. PMID: 39294189 Free PMC article.
Identification of multipotent drugs for COVID-19 therapeutics with the evaluation of their SARS-CoV2 inhibitory activity.
Kumar S, Singh B, Kumari P, Kumar PV, Agnihotri G, Khan S, Kant Beuria T, Syed GH, Dixit A. Kumar S, et al. Comput Struct Biotechnol J. 2021;19:1998-2017. doi: 10.1016/j.csbj.2021.04.014. Epub 2021 Apr 7. Comput Struct Biotechnol J. 2021. PMID: 33841751 Free PMC article.
Evidence for the medicinal value of Squama Manitis (pangolin scale): A systematic review.
Jin X, Chua HZ, Wang K, Li N, Zheng W, Pang W, Yang F, Pang B, Zhang M, Zhang J. Jin X, et al. Integr Med Res. 2021 Mar;10(1):100486. doi: 10.1016/j.imr.2020.100486. Epub 2020 Jul 28. Integr Med Res. 2021. PMID: 32837905 Free PMC article. Review.

See all "Cited by" articles

References

1. Wu, F. et al. A new coronavirus associated with human respiratory disease in China. Nature 579, 265–269 (2020). - DOI
1. Lu, R. et al. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet 395, 565–574 (2020). - DOI
1. World Health Organization. Coronavirus disease 2019 (COVID-19) Situation Report – 101 https://www.who.int/docs/default-source/coronaviruse/situation-reports/2... v.101 (30 April 2020).
1. Cohen, J. Mining coronavirus genomes for clues to the outbreak’s origins. Science https://www.sciencemag.org/news/2020/01/mining-coronavirus-genomes-clues... (31 January 2020).
1. Wang, M. et al. SARS-CoV infection in a restaurant from palm civet. Emerg. Infect. Dis. 11, 1860–1865 (2005). - DOI

Publication types

Actions
Actions
Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions

Grants and funding

HHSN272201400006C/AI/NIAID NIH HHS/United States

LinkOut - more resources

Full Text Sources
- Nature Publishing Group
Other Literature Sources
- H1 Connect
- The Lens - Patent Citations Database
Miscellaneous
- NCI CPTAC Assay Portal

[1] Wu, F. et al. A new coronavirus associated with human respiratory disease in China. Nature 579, 265–269 (2020). - DOI

[2] Wu, F. et al. A new coronavirus associated with human respiratory disease in China. Nature 579, 265–269 (2020). - DOI

[3] Lu, R. et al. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet 395, 565–574 (2020). - DOI

[4] Lu, R. et al. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet 395, 565–574 (2020). - DOI

[5] World Health Organization. Coronavirus disease 2019 (COVID-19) Situation Report – 101 https://www.who.int/docs/default-source/coronaviruse/situation-reports/2... v.101 (30 April 2020).

[6] World Health Organization. Coronavirus disease 2019 (COVID-19) Situation Report – 101 https://www.who.int/docs/default-source/coronaviruse/situation-reports/2... v.101 (30 April 2020).

[7] Cohen, J. Mining coronavirus genomes for clues to the outbreak’s origins. Science https://www.sciencemag.org/news/2020/01/mining-coronavirus-genomes-clues... (31 January 2020).

[8] Cohen, J. Mining coronavirus genomes for clues to the outbreak’s origins. Science https://www.sciencemag.org/news/2020/01/mining-coronavirus-genomes-clues... (31 January 2020).

[9] Wang, M. et al. SARS-CoV infection in a restaurant from palm civet. Emerg. Infect. Dis. 11, 1860–1865 (2005). - DOI

[10] Wang, M. et al. SARS-CoV infection in a restaurant from palm civet. Emerg. Infect. Dis. 11, 1860–1865 (2005). - DOI

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Identifying SARS-CoV-2-related coronaviruses in Malayan pangolins

Affiliations

Identifying SARS-CoV-2-related coronaviruses in Malayan pangolins

Authors

Affiliations

Abstract

Comment in

Similar articles

Cited by

References

Publication types

MeSH terms

Substances

Grants and funding

LinkOut - more resources

Full Text Sources

Other Literature Sources

Miscellaneous

Abstract

Comment in

Similar articles

Cited by

References

Publication types

MeSH terms

Substances

Related information

Grants and funding

LinkOut - more resources

Full Text Sources

Other Literature Sources

Miscellaneous