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The images were observed under a fluorescence microscope at ×200. EC9706 cells (B), KYSE150 cells (C) or NIH 3T3 cells (D) were exposed to the indicated concentrations of Ec-LDP-Hr-AE for 48 h. Cells were harvested and stained with a combination of FITC-Annexin V and PI. The lower left quadrants (FITC−/PI−) indicated the viable cells, and the lower right quadrants (FITC+/PI−) indicated the early apoptotic cells. 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