English: A schematic view of primers and _target region for PCR amplification, demonstrated on 16S rRNA gene in Pseudomonas. Primers are short conserved sequences with low variability that can _target most or all species in the chosen _target group. They are used to amplify highly variable _target region in between two primers, which is the basis for species discrimination. Modified from »Variable Copy Number, Intra-Genomic Heterogeneities and Lateral Transfers of the 16S rRNA Gene in Pseudomonas - Scientific Figure on ResearchGate« by Bodilis, Josselin; Nsigue-Meilo, Sandrine; Besaury, Ludovic; Quillet, Laurent, used under CC BY, available from: https://www.researchgate.net/figure/Hypervariable-regions-within-the-16S-rRNA-gene-in-Pseudomonas-The-plotted-line-reflects_fig2_224832532.