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ADHD Animal Model Characterization: Transcriptomics and Proteomics Analyses

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Psychiatric Disorders

Part of the book series: Methods in Molecular Biology ((MIMB,volume 829))

Abstract

Mechanisms underlying behavioral abnormalities of patients with attention-deficit hyperactivity disorder (ADHD) disorder are still unknown. It is worth clarifying alterations in the brain of animal models for ADHD. The animals with neonatal treatment with 6-hydroxydopamine (6-OHDA) and congenic wiggling (Wig) rats show motor hyperactivities during a period of darkness at 4 weeks of age. In rats with 6-OHDA lesions, subcutaneous injection of methamphetamine attenuates hyperactivity, the reverse of its effect in Wig rats. To understand mechanisms underlying such behavioral abnormalities, transcriptomics and proteomics analyses may provide novel information in brain research. The expression of genes and proteins in brain regions can be measured by DNA microarray and two-dimensional gel electrophoresis, respectively. We have shown different expressions of genes and proteins in brains of rats with neonatal 6-OHDA lesions and Wig rats.

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Acknowledgments

This work was supported in part by a fellowship to Y.M. from the New Energy and Industrial Technology Development Organization (NEDO) and by the Ministry of the Environment; and the Ministry of Economy, Trade, and Industry, Japan. We appreciate the support of Drs. Hyung Wook Nam (Protein Network Research Center, Yonsei University, Seoul, Korea), Yoko Ogawa and Yasukazu Yoshida (Health Technology Research Center, AIST, Ikeda, Osaka, Japan) in use of the MALDI-MS and LC-MS/MS equipments. RR also appreciates the kind support of Prof. Seiji Shioda (Department of Anatomy I, Showa University-School of Medicine) for his encouragement on multidisciplinary research and study.

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Authors and Affiliations

  1. Laboratory of Neuroscience, Department of Biology, Toho University, Funabashi, Chiba, Japan

    Yoshinori Masuo & Junko Shibato

  2. School of Medicine, Showa University, Shinagawa, Tokyo, Japan

    Randeep Rakwal

Authors
  1. Yoshinori Masuo
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  2. Junko Shibato
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  3. Randeep Rakwal
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Corresponding author

Correspondence to Yoshinori Masuo .

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Editors and Affiliations

  1. McKnight Brain Institute, Department of Psychiatry, University of Florida, S. Newell Dr. 100, Gainesville, 32610, Florida, USA

    Firas H. Kobeissy

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Masuo, Y., Shibato, J., Rakwal, R. (2012). ADHD Animal Model Characterization: Transcriptomics and Proteomics Analyses. In: Kobeissy, F. (eds) Psychiatric Disorders. Methods in Molecular Biology, vol 829. Humana Press. https://doi.org/10.1007/978-1-61779-458-2_32

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  • DOI: https://doi.org/10.1007/978-1-61779-458-2_32

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-457-5

  • Online ISBN: 978-1-61779-458-2

  • eBook Packages: Springer Protocols

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