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Comparative Effect of Chlorhexidine and Some Mouthrinses on Bacterial Biofilm Formation on Titanium Surface

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Abstract

The aim of the present study was to evaluate the effectiveness of chlorhexidine digluconate (CHX) and commonly used mouthrinses to single- and poly-species biofilms by S. mutans, S. aureus and P. aeruginosa, on titanium discs of grade IV. The formation of single- and poly-species biofilms at 16.5, 40.5 and 64.5-h incubation on titanium surface was evaluated by plate count (CFU ml−1) before and after exposure to CHX and four mouthrinses (Curasept, Listerine, Meridol and Buccagel) and expressed as percentage of Inhibitory Activity (IA%). The application of the different anti-plaque formulations on biofilm can reduce the adhesion of bacteria to titanium surface with different degrees. The higher efficacy was observed for Listerine that shows IA% = 100 on the biofilm formed by S. mutans at 16.5 h. Log count of CFU was dependent to culture time and four mouthrinses for S. mutans and S. aureus, whilst was not dependent to culture time but to mouthrinses for P. aeruginosa. In general, the efficacy was particularly lesser to poly-species biofilms; no statistical differences were evidenced between all the mouthrinses and CHX as control group. The tested mouthrinses, compared to reference CHX 0.2%, have demonstrated a significant lower antibacterial activity than Listerine towards the experimental biofilms. This “in vitro” biofilm model should prove extremely useful for pre-clinical testing of anti-plaque agents, which inhibit biofilm formation, can prevent subsequent implant failure.

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Authors and Affiliations

  1. Department of Biomolecular Science, Division of Toxicological, Hygienistic and Environmental Sciences, University of Urbino “Carlo Bo”, Via Santa Chiara, 27, Urbino, Italy

    Wally Baffone & Raffaella Campana

  2. Department of Dentistry, University of Rome “Tor Vergata”, Rome, Italy

    Gianfranco Sorgente

  3. Center of Biotechnology Researches, University of Cremona “Sacro Cuore”, Cremona, Italy

    Vania Patrone

  4. DiSUAN Department, Division of Biomathematic, University of Urbino “Carlo Bo”, Urbino, Italy

    Davide Sisti

  5. Department de Producció Vegetal i Ciència Forestal, Universitat de Lleida, Avda. A. Rovira Roure 191, 25198, Lleida, Spain

    Tania Falcioni

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  1. Wally Baffone
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  2. Gianfranco Sorgente
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  3. Raffaella Campana
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  4. Vania Patrone
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  5. Davide Sisti
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  6. Tania Falcioni
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Correspondence to Wally Baffone.

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Baffone, W., Sorgente, G., Campana, R. et al. Comparative Effect of Chlorhexidine and Some Mouthrinses on Bacterial Biofilm Formation on Titanium Surface. Curr Microbiol 62, 445–451 (2011). https://doi.org/10.1007/s00284-010-9727-x

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  • DOI: https://doi.org/10.1007/s00284-010-9727-x

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