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Brassica rapa var. japonica Leaf Extract Mediated Green Synthesis of Crystalline Silver Nanoparticles and Evaluation of Their Stability, Cytotoxicity and Antibacterial Activity

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Abstract

Silver nanoparticles (AgNPs) were successfully synthesized from the reduction of Ag+ using AgNO3 solution as a precursor and Brassica rapa var. japonica leaf extract as a reducing and capping agent. This study was aimed at synthesis of AgNPs, exhibiting less toxicity with high antibacterial activity. The characterization of AgNPs was carried out using UV–Vis spectrometry, energy dispersive X-ray spectrometry, fourier transform infrared spectrometry, field emission scanning electron microscopy, X-ray diffraction, atomic absorption spectrometry, and transmission electron microscopy analyses. The analyses data revealed the successful synthesis of nano-crystalline Ag possessing more stability than commercial AgNPs. The cytotoxicity of Brassica AgNPs was compared with commercial AgNPs using in vitro PC12 cell model. Commercial AgNPs reduced cell viability to 23% (control 97%) and increased lactate dehydrogenase activity at a concentration of 3 ppm, whereas, Brassica AgNPs did not show any effects on both of the cytotoxicity parameters up to a concentration level of 10 ppm in PC12 cells. Moreover, Brassica AgNPs exhibited antibacterial activity in terms of zone of inhibition against E. coli (11.1 ± 0.5 mm) and Enterobacter sp. (15 ± 0.5 mm) which was higher than some previously reported green-synthesised AgNPs. Thus, this finding can be a matter of interest for the production and safe use of green-AgNPs in consumer products.

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References

  1. S. Gajbhiye, S. Sakharwade, J. Cosmet. Dermatol. Sci. Appl. 6, 48–53 (2016)

    CAS  Google Scholar 

  2. M. Akter, M.T. Sikder, M.M. Rahman, A.K.M.A. Ullah, K.F.B. Hossain, M. Kurasaki, J. Adv. Res. 9, 1–16 (2018)

    Article  CAS  PubMed  Google Scholar 

  3. J.G. Rouse, J. Yang, J.P. Ryman-Rasmussen, A.R. Barron, N.A. Monteiro-Riviere, Nano Lett. 7, 155–160 (2007)

    Article  CAS  PubMed  Google Scholar 

  4. T. Prow, J.E. Grice, L. Lin, F.N. Rokhaya, M. Butler, W. Becker et al., Adv. Drug. Deliv. Rev. 63, 470–491 (2011)

    Article  CAS  PubMed  Google Scholar 

  5. M. Kreilgaard, Adv. Drug. Deliv. Rev. 54, 77–98 (2002)

    Article  Google Scholar 

  6. S. Kokura, O. Handa, T. Takagi, T. Ishikawa, Y. Naito, T. Yoshikawa, Nanomedicine 6, 570–574 (2010)

    Article  CAS  PubMed  Google Scholar 

  7. Z. Shavandi, T. Ghazanfari, K.N. Moghaddam, Immunopharmacol. Immunotoxicol. 33, 135–140 (2011)

    Article  CAS  PubMed  Google Scholar 

  8. J.H. Sung, J.H. Ji, J.D. Park, J.U. Yoon, D.S. Kim, K.S. Jeon, Toxicol. Sci. 108, 452–461 (2009)

    Article  CAS  PubMed  Google Scholar 

  9. F. Sambale, S. Wagner, F. Stahl, R.R. Khaydarov, T. Scheper, D. Bahnemann, J. Nanomater. (2015). https://doi.org/10.1155/2015/136765

    Article  Google Scholar 

  10. J. Mytych, J. Zebrowski, A. Lewinska, M. Wnuk, Mol. Neurobiol. 54, 1–16 (2016)

    Google Scholar 

  11. M.G. Guzmán, J. Dille, S. Godet, Int. J. Chem. Biomol. Eng. 2, 104–111 (2009)

    Google Scholar 

  12. C. Mao, Y. Xiang, X. Liu, Z. Cui, X. Yang, K.W. Yeung, H. Pan, X. Wang, P.K. Chu, S. Wu, ACS Nano 11, 9010–9021 (2017)

    Article  CAS  PubMed  Google Scholar 

  13. M. Starowicz, B. Stypula, J. Banas, Electrochem. Commun. 8, 227–230 (2006)

    Article  CAS  Google Scholar 

  14. H. Xu, B.W. Zeiger, K.S. Suslick, Chem. Soc. Rev. 42, 2555–2567 (2013). https://doi.org/10.1039/C2CS35282F

    Article  CAS  PubMed  Google Scholar 

  15. P. Velmurugan, S.C. Hong, A. Aravinthan, S.H. Jang, P.I. Yi, Y.C. Song et al., Arab. J. Sci. Eng. 42, 201–208 (2016)

    Article  CAS  Google Scholar 

  16. J.L. Gardea-Torresdey, E. Gomez, J.G. Parsons, J.R. Peralta-Videa, J.G. Parsons, H.E. Troiani, J. Langmuir. 19, 1357–1361 (2003)

    Article  CAS  Google Scholar 

  17. S.P. Chandran, M. Chaudhary, R. Pasricha, A. Ahmad, M. Sastry, Biotechnol Prog. 22, 577–583 (2006)

    Article  CAS  PubMed  Google Scholar 

  18. J.M. Ashraf, M.A. Ansari, H.M. Khan, A.M.A. Alzohairy, I. Choi, Choi, Sci. Rep. (2016). https://doi.org/10.1038/srep20414

    Article  PubMed  PubMed Central  Google Scholar 

  19. A. Narayanamma, IOSR J. Polym. Text. Eng. 3, 17–25 (2016)

    Google Scholar 

  20. S.M. Roopan, G. Madhumitha, A.A. Rahuman, C. Kamaraj, A. Bharathi, T.V. Surendra, Ind. Crops. Prod. 43, 631–635 (2013)

    Article  CAS  Google Scholar 

  21. U.B. Jagtap, V.A. Bapat, J. Plant. Biochem. Biotechnol. 22, 434–440 (2013)

    Article  CAS  Google Scholar 

  22. G.A. Kahrilas, L.M. Wally, S.J. Fedrick, M. Hiskey, A.L. Prieto, E. Janel, ACS Sustain. Chem. Eng. 2, 367–376 (2014)

    Article  CAS  Google Scholar 

  23. F.H. Sahin, T. Aktas, F.E. Acikgoz, T. Akcay, Peer J. Pre Prints (2016) https://doi.org/10.7287/peerj.preprints.1698v1

    Article  Google Scholar 

  24. C. Jiang. Y. Yuan, F. Hu, Q. Wang, K. Zhang, Y. Wang et al., Biol. Trace Elem. Res. 158, 249–258 (2014)

    Article  CAS  PubMed  Google Scholar 

  25. S.C. Edberg, E.W. Rice, R.J. Karlin, M.J. Allen, Appl Microbiol. 88, 1068–1168 (2000)

    Article  Google Scholar 

  26. X. Xie, C. Mao, X. Liu, Y. Zhang, Z. Cui, X. Yang, K.W.K. Yeung, H. Pan, P.K. Chu, S. Wu, ACS Appl. Mater. Interfaces 9, 26417–26428 (2017)

    Article  CAS  PubMed  Google Scholar 

  27. Y. Zhang, X. Liu, Z. Li, S. Zhu, X. Yuan, Z. Cui, X. Yang, P.K. Chu, S. Wu, ACS Appl. Mater. Interfaces. https://doi.org/10.1021/acsami.7b17351

  28. K. Soo-Hwan, H.S. Lee, D.S. Ryu, S.J. Choi, D.S. Lee, Korean J. Microbiol. Biotechnol. 39, 77–85 (2011)

    Google Scholar 

  29. J.W. Chow, V.L. Yu, D.M. Shlaes, Am. J. Infect. Control. 22, 195–220 (1994)

    Article  CAS  PubMed  Google Scholar 

  30. M.M. Rahman, J. Ukiana, R.U. Lopez, M.T. Sikder, T. Saito, Chem. Biol. Interact. 269, 41–49 (2017)

    Article  CAS  PubMed  Google Scholar 

  31. M.M. Rahman, R.U. Lopez, M.T. Sikder, G. Tan, T. Hosokawa, T. Saito, M. Kurasaki, Chemosphere 196, 453–466 (2018)

    Article  CAS  PubMed  Google Scholar 

  32. M. Saravanan, A.K. Vemu, S.K. Barik, Colloids Surf. B Biointerfaces 88, 325–331 (2011)

    Article  CAS  PubMed  Google Scholar 

  33. Z. Liu, Y. Zhu, X. Liu, K.W.K. Yeung, S. Wu, Colloids Surf. B Biointerfaces 151, 165–177 (2017)

    Article  CAS  PubMed  Google Scholar 

  34. A.K.M.A. Ullah, A.K.M.F. Kibria, M. Akter, M.N.I. Khan, M.A. Maksud, R.A. Jahan, S.H. Firoz, J. Saudi Chem. Soc. 21, 830–836 (2017)

    Article  CAS  Google Scholar 

  35. G. Sagar, B. Ashok, Eur. J. Exp. Biol. 2, 1654–1658 (2012)

    CAS  Google Scholar 

  36. A.K.M.A. Ullah, A.K.M.F. Kibria, M. Akter, M.N.I. Khan, A.R.M. Tareq, S.H. Firoz, Water Conserv. Sci. Eng. 1, 249–256 (2017)

    Article  Google Scholar 

  37. H.M.B. Alam, R. Das, M. Shajahan, A.K.M.A. Ullah, A.K.M.F. Kibria, Int. J. Hydrog. Energy 43, 1998–2008 (2018)

    Article  CAS  Google Scholar 

  38. F. Yang, M. Zhao, B. Zheng, D. Xiao, L. Wu, Y. Guo, J. Matter. Chem. 22, 25471–25479 (2012)

    Article  CAS  Google Scholar 

  39. P.H.M. Hoet, I. Bruske-Hohlfeld, O.V. Salata, J. Nanobitechnol. 2, 1–15 (2004)

    Article  Google Scholar 

  40. E.J. Park, J. Yi, Y. Kim, K. Choi, K. Park, Toxicol. In Vitro 24, 872–878 (2010)

    Article  CAS  PubMed  Google Scholar 

  41. C. Krishnaraj, E.G. Jagan, S. Rajasekar, P. Selvakumar, P.T. Kalaichelvan, N. Mohan, Colloids Surf. B Biointerfaces 76, 50–56 (2010)

    Article  CAS  PubMed  Google Scholar 

  42. J. Huang, Q. Li, D. Sun, Y. Lu, Y. Su, X. Yang, Nanotechnology 18(11), 105104 (2007)

    Article  CAS  Google Scholar 

  43. K.T. Vadiraj, S.L. belagali, J. Mater. Sci. 27, 2885–2889 (2016)

    CAS  Google Scholar 

  44. G. Sathishkumar, C. Gobinath., K. Karpagam, V. Hemamalini, K. Premkumar, S. Sivaramakrishnan, Colloids Surf. B Biointerfaces 95, 235–240 (2012)

    Article  CAS  PubMed  Google Scholar 

  45. Y.H. Hsueh, K.S. Lin, W.J. Ke, C.T. Hsieh, C.L. Chiang, PLoS ONE (2015) https://doi.org/10.1371/journal.pone.0144306

    Article  PubMed  PubMed Central  Google Scholar 

  46. M. Jayaraj, G. Sathishkumar, G. Sivanandhan, A. Mubarak, M. Rajesh, R. Aurun, Colloids Surf. B Biointerfaces 106, 86–92 (2013)

    Article  CAS  Google Scholar 

  47. S.I. Kaba, E.M. Egorova, Nanotechnol. Sci. Appl. 8, 19–29 (2015)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  48. S. Kim, J.E. Choi, J. Choi, K.H. Chung, K. Park, Y. Jongheop, et al. Toxicol. In Vitro 23, 1076–1084 (2009)

    Article  CAS  PubMed  Google Scholar 

  49. S. Arora, J. Jain, J.M. Rajwade, K.M. Paknikar, Toxicol. Lett. 179, 93–100 (2008)

    Article  CAS  PubMed  Google Scholar 

  50. S.M. Hussain, K.L. Hess, J.M. Gearhart, K.T. Geiss, J.J. Schlager, Toxicol. In Vitro 19, 975–983 (2005)

    Article  CAS  PubMed  Google Scholar 

  51. R.M. Gengan, K. Anand, A. Phulukdaree, A. Chuturgoon, Colloids Surf. B Biointerfaces 105, 87–91 (2013)

    Article  CAS  PubMed  Google Scholar 

  52. M.P. Noghabi, M.R. Parizadeh, M.G. Mobarhan, D. Taherzadeh, H.A.H.M. Darroudi, J. Mol. Struct. 1146, 499–503 (2017)

    Article  CAS  Google Scholar 

  53. S.S. Saraswati, G.C. Kundu, A. Ahmad, Spectrochim. Acta A Mol. Biomol. Spectrosc. 114, 144–147 (2013)

    Article  CAS  PubMed  Google Scholar 

  54. P. Velmurugan., M. Iydroose., M.H. Mohideen. T.S. Mohan, M. Cho, B.T. Oh, Bioprocess. Biosyst. Eng. 37, 1527–1534 (2014)

    Article  CAS  PubMed  Google Scholar 

  55. K. Renugadevi, R.V. Aswani, Int. J. Nanomater. Biostruct. 2, 5–10 (2012)

    Google Scholar 

  56. A.A. Abdullah, Z.U. Hamid, J. Biol. Agric. Healthc. 3, 67–71 (2013)

    Google Scholar 

  57. Q. Sun, X. Cai, J. Li, M. Zheng, Z. Chen, C.P. Yu, Colloids Surf. A Physicochem. Eng. Asp. 444, 226–231 (2014)

    Article  CAS  Google Scholar 

  58. A.R. Allafchian, S.Z. Mirahmadi-Zare, S.A.H. Jalali, S.S. Hashemi, M.R. Vahabi, J. Nanostruct. Chem. 6, 129–135 (2016)

    Article  CAS  Google Scholar 

  59. I. Maliszewska., Z. Sadowski, J. Phys. 2009, 146 (2009). https://doi.org/10.1088/1742-6596/146/1/012024

    Article  CAS  Google Scholar 

  60. J.R. Morones, A.C. Elechifuerra, K. Holt, J.B. Kouri, J.T. Ramirez, M.J. Yacaman, Nanotechnology 16, 2346–2353 (2005)

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We are indebted to Mrs. Miyako Komori, Prof. Kamiya, Dr. Umezawa, and Dr. Parvin Begum, for giving instrumental support during experiments.

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Authors and Affiliations

  1. Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan

    Mahmuda Akter, Md. Mostafizur Rahman & Masaaki Kurasaki

  2. Chemistry Division, Atomic Energy Centre, Bangladesh Atomic Energy Commission, Dhaka 1000, Bangladesh

    A. K. M. Atique Ullah

  3. Faculty of Health Sciences, Hokkaido University, Sapporo, Japan

    Md. Tajuddin Sikder & Takeshi Saito

  4. Faculty of Environmental Earth Science, Hokkaido University, Kita 10, Nishi 5, Kita-ku, Sapporo 060-0810, Japan

    Md. Tajuddin Sikder & Masaaki Kurasaki

  5. Department of Public Health and Informatics, Jahangirnagar University, Dhaka, Bangladesh

    Md. Tajuddin Sikder

  6. Research Division of Higher Education, Institute for the Advancement of Higher Education, Hokkaido University, Sapporo 060-0817, Japan

    Toshiyuki Hosokawa

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  1. Mahmuda Akter
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Akter, M., Rahman, M.M., Ullah, A.K.M.A. et al. Brassica rapa var. japonica Leaf Extract Mediated Green Synthesis of Crystalline Silver Nanoparticles and Evaluation of Their Stability, Cytotoxicity and Antibacterial Activity. J Inorg Organomet Polym 28, 1483–1493 (2018). https://doi.org/10.1007/s10904-018-0818-7

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