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Proteomics for Protein Expression Profiling in Neuroscience

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Abstract

As the technology of proteomics moves from a theoretical approach to a practical reality, neuroscientists will have to determine the most appropriate applications for this technology. Neuroscientists will have to surmount difficulties particular to their research, such as limited sample amounts, heterogeneous cellular compositions in samples, and the fact that many proteins of interest are rare, hydrophobic proteins. This review examines protein isolation and protein fractionation and separation using two-dimensional electrophoresis (2-DE) and mass spectrometry proteomic methods. Methods for quantifying relative protein expression between samples (e.g., 2-DIGE, and ICAT) are also described. The coverage of the proteome, ability to detect membrane proteins, resource requirements, and quantitative reliability of different approaches is also discussed. Although there are many challenges in proteomic neuroscience, this field promises many rewards in the future.

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Authors and Affiliations

  1. Departments of Pharmacology and Psychiatry & Behavioral Sciences, Yerkes National Primate Research Center, Neuroscience Division, Emory University School of Medicine, Atlanta, Georgia

    Willard M. Freeman & Scott E. Hemby

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  1. Willard M. Freeman
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  2. Scott E. Hemby
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Correspondence to Scott E. Hemby.

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Freeman, W.M., Hemby, S.E. Proteomics for Protein Expression Profiling in Neuroscience. Neurochem Res 29, 1065–1081 (2004). https://doi.org/10.1023/B:NERE.0000023594.21352.17

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  • DOI: https://doi.org/10.1023/B:NERE.0000023594.21352.17

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