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Gene expression profiles of laser-captured adjacent neuronal subtypes

Nature Medicine volume 5, pages 117–122 (1999)Cite this article

A Correction to this article was published on 01 March 1999

Gene expression profiles of thousands of genes can now be examined en masse through cDNA and oligonucleotide microarrays1,2,3. Recently, studies have been reported that examined gene expression changes in yeast4,5, as well as in mammalian cell lines6, primary cells7 and tissues8. However, present applications of microarray technology do not include the study of gene expression from individual cell types residing in a given tissue/organ (that is, in situ). Such studies would greatly facilitate our understanding of the complex interactions that exist in vivo between neighboring cell types in normal and disease states. We demonstrate here that gene expression profiles from adjacent cell types can be successfully obtained by integrating the technologies of laser capture microdissection9 (LCM) and T7-based RNA amplification10 with cDNA microarrays11.

Neighboring small and large neurons are individually captured

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Figure 1: Laser capture microdissection from Nissl-stained sections (10 μm in thickness) of adult rat large and small DRG neurons.
Figure 2: cDNA microarray expression patterns of small (S) and large (L) neurons.
Figure 3: Representative fields of radioisotopic in situ hybridization of rat DRG with selected cDNAs.

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Acknowledgements

We thank K. Witmeyer, K. Flores, J. Chambers, A. Leung and V. Le for their technical assistance.

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  1. merlande@prius.jnj.com

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  1. R.W. Johnson Pharmaceutical Research Institute, 3535 General Atomics Court, Suite 100, San Diego, 92121, California, USA

    Lin Luo, Ranelle C. Salunga, Hongqing Guo, Anton Bittner, K.C. Joy, Jose E. Galindo, Huinian Xiao, Jackson S. Wan, Michael R. Jackson & Mark G. Erlander

  2. R.W. Johnson Pharmaceutical Research Institute, Spring House, 19477, Pennsylvania, USA

    Kathryn E. Rogers

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Luo, L., Salunga, R., Guo, H. et al. Gene expression profiles of laser-captured adjacent neuronal subtypes . Nat Med 5, 117–122 (1999). https://doi.org/10.1038/4806

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