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Lipids and cardiovascular/metabolic health

Postprandial total and HMW adiponectin following a high-fat meal in lean, obese and diabetic men

European Journal of Clinical Nutrition volume 67, pages 377–384 (2013)Cite this article

Subjects

Abstract

Background/Objectives:

Recent work suggests that macronutrients are pro-inflammatory and promote oxidative stress. Reports of postprandial regulation of total adiponectin have been mixed, and there is limited information regarding postprandial changes in high molecular weight (HMW) adiponectin. The aim of this study was to assess the effect of a standardised high-fat meal on metabolic variables, adiponectin (total and HMW), and markers of inflammation and oxidative stress in: (i) lean, (ii) obese non-diabetic and (iii) men with type 2 diabetes mellitus (T2DM).

Subjects/Methods:

Male subjects: lean (n=10), obese (n=10) and T2DM (n=10) were studied for 6 h following both a high-fat meal and water control. Metabolic variables (glucose, insulin, triglycerides), inflammatory markers (interleukin-6 (IL6), tumour necrosis factor (TNF)α, high-sensitivity C-reactive protein (hsCRP), nuclear factor (NF)κB expression in peripheral blood mononuclear cells (p65)), indicators of oxidative stress (oxidised low density lipoprotein (oxLDL), protein carbonyl) and adiponectin (total and HMW) were measured.

Results:

No significant changes in TNFα, p65, oxLDL or protein carbonyl concentrations were observed. Overall, postprandial IL6 decreased in subjects with T2DM but increased in lean subjects, whereas hsCRP decreased in the lean cohort and increased in obese subjects. There was no overall postprandial change in total or HMW adiponectin in any group. Total adiponectin concentrations changed over time following the water control, and the response was significantly different in lean subjects compared with subjects with T2DM (P=0.04).

Conclusions:

No consistent significant postprandial inflammation, oxidative stress or regulation of adiponectin was observed in this study. Findings from the water control suggest differential basal regulation of total adiponectin in T2DM compared with lean controls.

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Acknowledgements

We thank Mr Goce Dimeski, Clinical Biochemistry Princess Alexandra Hospital, Brisbane, Australia, for his help with biochemical analyses; Louise Cooney, Diamantina Institute, the University of Queensland, Brisbane, Australia, for her help with clinical studies. This research was supported by participation from the 50+ Registry of the Australasian Centre on Ageing, the University of Queensland, Brisbane, Australia. The following grants and fellowships supported the writing of this paper: National Health and Medical Research Council, Australia; Princess Alexandra Foundation, Brisbane, Australia; GlaxoSmithKline Postgraduate Support Grant.

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Authors and Affiliations

  1. Diamantina Institute, Princess Alexandra Hospital, University of Queensland, Brisbane, Queensland, Australia

    L K Phillips & I J Hickman

  2. Faculty of Health, School of Biomedical Sciences, Queensland University of Technology, Brisbane, Queensland, Australia

    J M Peake

  3. Department of Gastroenterology and Hepatology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China

    X Zhang

  4. School of Human Movement Studies, The University of Queensland, Brisbane, Queensland, Australia

    D R Briskey

  5. CSIRO Mathematics, Informatics and Statistics, Queensland Bioscience Precinct, Brisbane, Queensland, Australia

    B E Huang

  6. Department of Pediatrics, Section of Quantitative Health Sciences, Medical College of Wisconsin, Milwaukee, WI, USA

    P Simpson & S-H Li

  7. Mater Medical Research Institute and University of Queensland, Brisbane, Queensland, Australia

    J P Whitehead & J B Prins

  8. Clinical School of Medicine, Princess Alexandra Hospital, University of Queensland, Brisbane, Queensland, Australia

    J H Martin

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Phillips, L., Peake, J., Zhang, X. et al. Postprandial total and HMW adiponectin following a high-fat meal in lean, obese and diabetic men. Eur J Clin Nutr 67, 377–384 (2013). https://doi.org/10.1038/ejcn.2013.49

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