Methods for error correction and classification of metagenomic datasets suggest that the rare biosphere is not as large as previously assumed.
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References
Brady, A. & Salzberg, S.L. Nat. Methods 6, 673–676 (2009).
Quince, C. et al. Nat. Methods 6, 639–641 (2009).
Turnbaugh, P.J. et al. Nature 457, 480–484 (2009).
Sogin, M.L. et al. Proc. Natl. Acad. Sci. USA 103, 12115–12120 (2006).
Roesch, L.F. et al. ISME J. 1, 283–290 (2007).
Fierer, N., Hamady, M., Lauber, C.L. & Knight, R. Proc. Natl. Acad. Sci. USA 105, 17994–17999 (2008).
Tringe, S.G. & Hugenholtz, P. Curr. Opin. Microbiol. 11, 442–446 (2008).
Huse, S.M., Huber, J.A., Morrison, H.G., Sogin, M.L. & Welch, D.M. Genome Biol. 8, R143 (2007).
McHardy, A.C., Martin, H.G., Tsirigos, A., Hugenholtz, P. & Rigoutsos, I. Nat. Methods 4, 63–72 (2007).
Ley, R.E. et al. Science 320, 1647–1651 (2008).
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Reeder, J., Knight, R. The 'rare biosphere': a reality check. Nat Methods 6, 636–637 (2009). https://doi.org/10.1038/nmeth0909-636
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DOI: https://doi.org/10.1038/nmeth0909-636
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