The Effect and Biocompatibility of Nanofibrous Nano-Hydroxyapatite/Polycaprolactone/Gelatin Scaffolds Multipurpose Membrane in Guiding Bone Regeneration

To prepare a new tri-layer multifunctional membrane (TLMM), which comprises gelatin loaded with tinidazole as the outer layer, dense polycaprolactone (PCL) as the intermediate layer, and compound nHA/PCL/gelatin as the inner layer, and to investigate its applicability as a barrier membrane to guide osteanagenesis. The morphology of TLMM prepared is observed using a scanning electron microscope (SEM). The hydrophilicity and swelling ratio of are tested. The mechanical properties of TLMM are tested using a universal electronic tester. The infrared spectrogram of each component in TLMM is obtained using infrared Fourier spectrometer through analytic transmission method. MG63 cell is cultivated on the surface of the multifunctional membrane, the activity of ALP is tested, and the cellular vitality is determined through the cck-8 test. Rats with skull defect are used as the test model. The skull recovery of the rats with skull defect is examined through Micro-CT method. The expression of osteocalcin and osteopontin in the cranial tissue of rats whose skull defect is repaired through TLMM repair is tested through WB test. SEM shows that this compound membrane has a three-dimensional network structure of nanofiber, and the cross-section of the membrane has a thickness of 120 m. TLMM has good hydrophilicity and extremely low swelling ratio. Results from mechanical testing show that TLMM has excellent mechanical strength. Infrared spectrum result demonstrates that the gelatin successfully carries tinidazole, and the release mechanism of tinidazole matches the recovery process after surgery. CCK-8 test indicates that TLMM has no significant influence on the proliferation of MG63 cell, but can greatly improve the activity of cellular ALP. Micro-CT examination proves that TLMM can effectively facilitate cranial recovery among rats with skull defect. WB test shows that TLMM can significantly induce the expression of osteocalcin and osteopontin in the cranial tissue of rats with skull defect, and performs better than traditional bone powder. The results demonstrate that TLMM designed by the study has good biocompatibility, and is applicable as a barrier membrane to guide osteanagenesis.