Next Article in Journal
Comparison and Three-Dimensional Fluorescence Spectrum Analysis of Activated Sludge Treatment with Fenton and UV-Fenton
Next Article in Special Issue
Genomic Insights into the Symbiotic and Plant Growth-Promoting Traits of “Candidatus Phyllobacterium onerii” sp. nov. Isolated from Endemic Astragalus flavescens
Previous Article in Journal
Differences in Bacterial Co-Occurrence Networks and Ecological Niches at the Surface Sediments and Bottom Seawater in the Haima Cold Seep
Previous Article in Special Issue
Metagenomic Approach Deciphers the Role of Community Composition of Mycobiome Structured by Bacillus velezensis VB7 and Trichoderma koningiopsis TK in Tomato Rhizosphere to Suppress Root-Knot Nematode Infecting Tomato
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Microorganisms
Volume 11
Issue 12
10.3390/microorganisms11123002
microorganisms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Communication

Soil Bacterial Diversity Responds to Long-Term Establishment of Perennial Legumes in Warm-Season Grassland at Two Soil Depths

by
Adesuwa Sylvia Erhunmwunse
1,2,*,
Victor Alonso Guerra
1,2,
Jung-Chen Liu
1,2,
Cheryl L. Mackowiak
1,2,
Ann Rachel Soffes Blount
1,
José Carlos Batista Dubeux, Jr.
3 and
Hui-Ling Liao
1,2
1
North Florida Research and Education Center, University of Florida, 155 Research Road, Quincy, FL 32351, USA
2
Soil, Water and Ecosystem Sciences Department, University of Florida, Gainesville, FL 32611, USA
3
North Florida Research and Education Center, University of Florida, 3925 Highway 71, Marianna, FL 32446, USA
*
Author to whom correspondence should be addressed.
Microorganisms 2023, 11(12), 3002; https://doi.org/10.3390/microorganisms11123002
Submission received: 31 October 2023 / Revised: 24 November 2023 / Accepted: 14 December 2023 / Published: 18 December 2023
(This article belongs to the Special Issue Research on Plant—Bacteria Interactions)
Browse Figures
Versions Notes

Abstract

:
The introduction of rhizoma peanut (RP Arachis glabrata Benth) into bahiagrass (Paspalum notatum Flüggé) may require time to develop stable plant–soil microbe interactions as the microbial legacy of the previous plant community may be long-lasting. A previous study showed that <2 years of introducing rhizoma peanut into bahiagrass pastures minimally affected soil bacterial diversity and community composition. In this study, we compared the effects of the long-term inclusion of rhizoma peanut (>8 years) into bahiagrass on soil bacterial diversity and community composition against their monocultures at 0 to 15 and 15 to 30 cm soil depths using next-generation sequencing to _target bacterial 16S V3–V4 regions. We observed that a well-established RP–bahiagrass mixed stand led to a 36% increase in bacterial alpha diversity compared to the bahiagrass monoculture. There was a shift from a soil bacterial community dominated by Proteobacteria (~26%) reported in other bahiagrass and rhizoma peanut studies to a soil bacterial community dominated by Firmicutes (39%) in our study. The relative abundance of the bacterial genus Crossiella, known for its antimicrobial traits, was enhanced in the presence of RP. Differences in soil bacterial diversity and community composition were substantial between 0 to 15 and 15 to 30 cm soil layers, with N2-fixing bacteria belonging to the phylum Proteobacteria concentrated in 0 to 15 cm. Introducing RP into bahiagrass pastures is a highly sustainable alternative to mineral N fertilizer inputs. Our results provide evidence that this system also promotes greater soil microbial diversity and is associated with unique taxa that require further study to better understand their contributions to healthy pastures.

1. Introduction

The integration of legumes into grasslands has gained renewed attention due to their potential to lower nitrogen (N) fertilizer inputs, improve soil health, enhance nutrient cycling, and minimize environmental damage associated with the excessive use of inorganic N fertilizer [1,2,3]. Rhizoma peanut (Arachis glabrata Benth.; RP) is a tropical perennial legume well adapted to pasture systems in the southeastern United States. It persists under grazing [4]. Extensive research has been conducted on the agronomic and livestock benefits of introducing RP into bahiagrass (Paspalum notatum Flüggé) pastures, a major warm-season perennial grass also adapted to the southeastern United States [4,5,6,7,8]. When compared to bahiagrass receiving N fertilizer, RP inclusion into a pasture elevates forage nutritive value and animal performance [5,6,7].
Likewise, desirable soil microbial associations with RP have also been observed [9,10,11,12]. For example, the presence of RP in bahiagrass systems was associated with increases in soil microbial diversity and the relative abundance of soil microbes with potential N2-fixing and cycling abilities, such as Bradyrhizobium and members of the Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium clade [10,11]. Plants influence soil microbial communities mainly through rhizodeposition and plant litter accumulation [13]. The impacts of plants on soil microbes are dynamic, with outcomes that are likely to change as plant growth and development progresses [14,15]. Kulmatiski and Beard [16] demonstrated that plants require several years to assemble soil microbial communities and that the development of plant–soil–microbe feedback should be viewed in a longer-term context in perennial plant systems. Hannula et al. [17] reported that soil microbiomes are reversible and plant–soil–microbe feedback characteristics at an early plant development stage can change with time. In a recent study, we observed that the bacterial alpha and beta diversities and the relative abundance of individual taxa within the first two years of RP integration into an existing bahiagrass system were not different from those in bahiagrass monoculture [12]. Rhizoma peanut establishment is achieved through the vegetative propagation of rhizomes and requires two to three years for stands to fully establish [18,19]. As a result, this longer establishment period likely delays RP impacts on soil microbial communities.
Many microbial studies on RP have _targeted the upper 10 to 15 cm soil depth [9,10,11,12]. The surface soil likely harbors greater microbial density because of greater root densities and plant litter accumulation, which also results in greater soil carbon [20,21,22]. Microbial diversity has been linked to the sustainability and productivity of grasslands by enhancing ecosystem services such as nutrient cycling [23,24]. Microbial diversity and community composition have differed between 0 to 15 and 15 to 30 cm, showing the differentiation of these microbial communities between these layers [20,22]. In this study, we compared the bacterial diversity and community composition in bahiagrass–RP mixtures versus their monocultures at two soil depths. We hypothesized that compared to bahiagrass monoculture, a well-established bahiagrass–RP mixed stand will result in greater bacterial diversity and increase the relative abundance of key soil bacterial taxa, especially near the surface soil (0 to 15 cm depth). Our objectives were to determine the longer-term (>8 years) impact of RP introduction into bahiagrass on soil bacterial diversity and community composition at two soil depths (0 to 15 and 15 to 30 cm). Overall, our results will provide valuable insights into the bacterial groups and the potential functions promoted by RP establishment in bahiagrass stands.

2. Materials and Methods

2.1. Experimental Site

The study was conducted in 2020 at the University of Florida, North Florida Research and Education Center, Quincy, Florida (30°33′ N, 84°36′ W). The soil was classified as Orangeburg loamy sand series (fine-loamy, kaolinitic, thermic Typic Kandiudults) [25]. The average temperature in 2020 was 20.0 °C (6.7 and 31.7 °C min/max, respectively). Annual rainfall was 1781 mm in 2020 compared to the 30-year average of 1519 mm [26]. The experimental site was initiated in 2011 and consisted of eight treatments, including two bahiagrass entries, two RP entries, and their mixtures, arranged in a randomized complete block design with three replicates. Detailed information on site establishment and management through 2017 is reported by Santos et al. [6] and Santos et al. [27]. In this study, only three of the existing forage treatments, Ecoturf RP (Eco), Argentine bahiagrass (Arg), and their binary mixtures (Arg-Eco), were sampled. Each plot measured 9 m2 with 1.2 m-wide alleys between plots. The average soil fertility of the soil samples collected from each plot was measured as follows for 0–15 cm and 15–30 soil depths: pH (2:1 water: soil) = 5.9 and 5.8, total soil carbon = 12 and 6 g kg−1, CEC = 4.6 and 3.4 cmol+ kg−1, Mehlich-3 extractable P, K, and Mg of 28 and 16, 76 and 49, and 79 and 44 mg kg−1. In 2019, there was a rotary cut in each plot at the end of the growing season. In March 2020, the RP monoculture plots were staged in March and treated for winter weeds in April 2020. Imazapic was applied at a rate of 291 mL ha−1 (0.07 kg a.i. ha−1), and Clethodim was applied ten days later at a rate of 877 mL ha−1 (0.21 kg a.i. ha−1). The grass monoculture and binary mixture treatments were mowed but not treated with herbicides.

2.2. Soil Sampling and Analysis

Soil samples (combined bulk and rhizosphere) were collected in July 2020 using a hydraulic soil coring probe (Giddings Machine Company, Windsor, CO, USA). Four soil cores (4.4 cm dia.) were collected from two soil depths (0 to 15 and 15 to 30 cm) per plot and composited. Subsamples were placed in 15 mL centrifuge tubes, frozen at −80 °C for 48 h, freeze-dried for 24 h and stored at −80 °C until DNA extraction. Genomic DNA was extracted from soil samples following the DNeasy PowerSoil extraction kit instructions (Qiagen, Hilden, Germany). DNA quantity and quality were measured using NanoDropTM One (Thermo Scientific, USA) based on the absorbance ratios (A260/A280 and A260/A230). Bacterial 16S rRNA gene V3–V4 regions were amplified using primer pairs 341F/806R [28]. Amplicon libraries were prepared using two PCR amplification steps described by Chen et al. [29] and using a Labnet MultiGene Optimax Thermal Cycler (Labnet International Inc., Edison, NJ, USA). At the second PCR stage, barcode tags unique to individual samples were tagged to the tail of the reverse primer. PCR products from the first and second steps were purified using AMPure XP beads (Beckman Coulter, Inc., Indianapolis, IN, USA) following the manufacturer’s instructions and observed on 1% agarose gels. Generated amplicon libraries were pooled at 20 ng/µL and sent to Duke Center for Genomic and Computational Biology (GCB) for Illumina MiSeq sequencing (v3 300 bp, 13 Gb) and paired-end reads were generated.

2.3. Bioinformatic and Statistical Analysis

The prokaryotic 16S rRNA gene sequences were processed in QIIME2 [30]. Primers were removed using cutadapt version 3.4 in QIIME2 version 2021.4 [30]. DADA2 was used for quality filtering and removal of chimeric sequences. Sequences at 100% similarity were assigned to the same amplicon sequence variant (ASV). Silva 138.1 database (version 2020) was used to assign taxonomic information. Singletons, unassigned, and reads that were classified as mitochondria and chloroplast were removed.
The ASV table (100% sequence similarity) was rarefied at 5000 sequencing depth and used for alpha and beta diversity. Alpha diversity indices were measured in QIIME2 using the Shannon index and observed features and analyzed using linear mixed model in R version 4.2.1 [31]. Forage treatment and soil depths were considered fixed effects and block was used as the random effect. When the main effect of forage treatment was significant, pairwise comparisons were carried out using Tukey’s post hoc test. In addition to graphical observations, Shapiro–Wilk’s test was used to assess the normal distribution of the residuals of our variables, and Bartlett’s test was used to determine the equality of variance. Beta diversity was estimated using the Bray–Curtis dissimilarity-based principal coordinates analysis with the vegdist function in vegan package. A test for homogeneity of multivariate dispersions (PERMDISP) within treatments was conducted [32] using 9999 permutations. PERMDISP was performed using the betadisper function in the vegan version 2.6-4 [33], and homogenous dispersion was observed within treatments. Dissimilarities of soil bacterial community composition in response to forage treatments, soil depth, and their interaction were tested using nonparametric permutational multivariate ANOVA [34] using 9999 permutations with the Adonis function in the vegan package. Pairwise comparisons were made for significant main effect of forage treatment using ADONIS.

3. Results

3.1. Soil Bacterial Community Composition

Across all forage treatments and soil depths, dominant phyla included Firmicutes (39 ± 4.3%), Proteobacteria (25 ± 2.3%), Actinobacteriota (15 ± 1.5%), and Acidobacteriota (7 ± 0.6%). Phyla representing <5% relative abundance included Chloroflexi (3.1 ± 0.7%), Verrucomicrobiota (2.9 ± 0.3%), Planctomycetota (2.0 ± 0.3%), and Gemmatimonadota (1.4 ± 0.2%), and Myxococcota (1.0 ± 0.1) (Figure 1). Unassigned and rare (<0.5%) phyla accounted for 1.8 ± 1.1% of all sequences. Approximately 76% of total sequences were assigned at the genus level. Bacillus (27.1 ± 3.0; Firmicutes), Rhodoplanes (8.9 ± 1.0; Proteobacteria), Tumebacillus (3.2 ± 0.8; Firmicutes), Paenibacillus (3.1 ± 0.7; Firmicutes), and Massilia (3.0 ± 0.7; Proteobacteria) were among the major bacterial genera, with Bacillus and Rhodoplanes accounting for 36% of the total bacterial genera.

3.2. Soil Bacterial Diversity in Response to Forage Treatment and Soil Depth

There were no significant two-way interactions between forage treatment and soil depth on bacterial alpha diversity measured in terms of the observed features or Shannon diversity index, but forage treatment and soil depth significantly (p < 0.05) affected bacterial alpha diversity (Table 1). Among all forage treatments, Ecoturf RP (Eco) and Arg-Eco mixture exhibited greater bacterial alpha diversity than bahiagrass monoculture (Arg). Observed features and Shannon diversity were 20 and 6%, respectively, greater in 0 to 15 cm than in 15 to 30 cm.
Bacterial beta diversity showed that soil bacterial community composition was significantly (p < 0.05) affected by forage treatment, soil depth, and their interaction (Table 2; Figure S1). At the 0 to 15 cm soil depth, soil bacterial community composition was distinct within each forage treatment. However, at 15 to 30 cm soil depth, soil bacterial community composition in Eco was dissimilar from those in the mixture (Arg-Eco) and bahiagrass monoculture (Arg) (Figure S1). Both Arg and Arg-Eco plots had overlapping soil bacterial communities. Soil depth explained 28% of the variation in soil bacterial community composition compared to 14% explained by forage treatment (Table 2).

3.3. Core Microbiome and Soil Bacterial Biomarkers

Core microbiomes were identified based on the presence of the ASVs in 50% of the samples across forage treatments and soil depths. Amplicon sequence variants identified at genus level included Bacillus (Firmicutes, 27.1 ± 2.9%), Rhodoplanes (Proteobacteria, 8.9 ± 1.0%), Tumebacillus (Firmicutes, 3.3 ± 0.8%), Bradyrhizobium (Proteobacteria, 2.2 ± 0.3%), Candidatus Udaeobacter (Verrucomicrobiota, 2.3 ± 1.0%), and Streptomyces (Actinobacteriota, 2.1 ± 0.8%) (Figure S2). Effects of forage treatment, soil depth, and their interaction were determined on the relative abundances of the core microbiome (Table S1). Crossiella and Phenylobacterium were influenced by forage treatment (Figure S3A). Crossiella was greater in Eco than Arg and Arg-Eco, while Phenylobacterium was enhanced in plots containing RP (Eco and Arg-Eco). Bradyrhizobium, Crossiella, Lysinibacillus, Paenibacillus, Rhodanobacter, Sphingomonas, and Subgroup_2 (Phylum: Actinobacteria) were affected by soil depth (Table S1). Bradyrhizobium, Lysinibacillus, and Sphingomonas were enriched in 0 to 15 cm soil depth and others were dominant in 15 to 30 cm soil depth (Figure S3B). Only Tumebacillus was affected by the interaction of forage treatment and soil depth (Table S1), where Eco demonstrated a greater relative abundance than the mixture, followed by Arg (Figure S3C).
Lefse analysis detected two abundant bacterial genera within each forage treatment, with Lachnoclostridium and Anaerocolumna (Firmicutes) identified in Eco, Massilia (Proteobacteria) and Leifsonia (Actinobacteriota) in Arg-Eco, and Arthrobacter (Actinobacteriota) and Terribacillus (Firmicutes) in Arg (Figure 2A). Fourteen bacterial genera were identified across soil depths, with six in 0 to 15 cm and eight in 15 to 30 cm (Figure 2B). Apart from Kribbella (Actinobacteriota), bacterial genera belonging to Proteobacteria were mainly present at the 0 to 15 cm soil depth and included Skermanella, Mesorhizobium, Phyllobacterium, Inquilinaceae, and Methylorubrum. Bacterial genera identified at 15 to 30 cm soil depth were a mixture of phyla Actinobacteriota (Nonomuraea, Oryzihumus, and Streptacidiphilus), Firmicutes (Fictibacillus, Thermoactinomyces, and Turicibacter), and Proteobacteria (Nevskia and Pseudoduganella).

4. Discussion

4.1. Soil Bacterial Diversity and Community Response

Legumes incorporated into grasslands often enhance soil bacterial diversity and community composition directly through the supply of N from biological N2 fixation and the release of N-rich root exudates and plant litter [13,35,36]. Increased microbial diversity in agroecosystems has been linked to increases in ecosystem services, such as nutrient cycling and nutrient availability [24,37], as well as resilience to environmental disturbances [38,39]. In support of our hypothesis, the long-term (>8 years) incorporation of RP into a bahiagrass system enhanced soil bacterial diversity and the relative abundance of core bacterial taxa. This result is in contrast to a previous study [12], where <2 years of RP establishment in an existing bahiagrass system did not alter soil microbial diversity relative to the bahiagrass monoculture. Prior existing plant communities can have a strong effect on soil community composition. However, the association between introduced plant species and soil bacteria may change over time within the same ecosystem as plant growth and development advances [40,41]. Schmid et al. [15] showed that the effect of old/existing grassland communities on soil microbial communities prior to the reassembling of new plant communities may still be identifiable after 4 years. The authors elaborated that the impact of a plant community on soil communities is a gradual process and requires more time to fully develop. In comparison, Hannula et al. [17] discovered that the influence of prior existing Poaceae and Asteraceae species on soil bacterial communities faded quickly with the introduction of new Poaceae and Asteraceae species.
Ecoturf RP and its mixture with bahiagrass had greater bacterial alpha diversity than bahiagrass monoculture. Factors such as soil properties and environmental conditions can drive microbial community structure in agricultural systems. However, plant species with divergent above and belowground properties also can exert a strong selective effect on soil bacterial communities [13,35]. For example, a concurrent study showed that RP root + rhizomes biomass and N concentrations were greater than bahiagrass root + rhizomes [42]. Furthermore, we found a 71% increase in rhizoma peanut root + rhizome biomass from 2015/2016 [43] to 2020 [42] on the same study site with greater annual rainfall in 2016 and similar temperature across study years. Often, perennial plants tend to accumulate belowground biomass over several years.
Interestingly, the bacterial community shifted from being dominated by Proteobacteria, as reported in other RP studies [10,11,12,44], to Firmicutes in our study. Reasons for the shift are not well understood, but Firmicutes have been reported in older, stable agroecosystems [45]. Different plant species have been linked to unique microbial taxa due to differences in plant litter quality and root exudate composition [36,44]. Guerra et al. [10] showed that Bryobacter, which is associated more with abundant C substrates, dominated a bahiagrass system, while RP had a greater relative abundance of Crossiella. Similarly, in our study, the relative abundance of Crossiella was enhanced under Ecoturf RP plots. Crossiella has been documented to have antimicrobial properties that can inhibit soil-borne pathogens [46]. Crossiella might provide an indirect defense mechanism against pathogens [46,47]. In our study, we observed that bacterial taxa capable of metabolizing a wide range of plant polysaccharides were found in bahiagrass and Ecoturf RP plots. For example, in Ecoturf RP plots, anaerobic, spore-forming bacteria, Anaerocolumna and Lachnoclostridium were identified. These require amino acids/peptides as a source of N for the decomposition of plant materials [48,49]. In comparison, the bahiagrass plots contained non-spore-forming and siderophore-producing Arthrobacter and aerobic Terribacillus, [50,51] known to utilize different organic compounds and thought to be involved in the transformation of soil organic matter [52].

4.2. Soil Depth Influences Variation in Soil Microbial Communities

Soil depth had a noticeable influence in shaping soil bacterial communities, as evident in the 28% variation of soil bacterial communities explained by soil depth. The impact of soil depth on soil bacterial communities has been attributed to changes in plant root systems and soil physicochemical properties, including soil C, soil moisture, and soil temperature, within soil depths [22,53]. Soil bacterial alpha diversity between 0 to 15 and 15 to 30 cm soil depths showed that 0 to 15 cm had greater bacterial diversity. It is well-established that microbial diversity is greater in the surface soil due to greater accumulation of organic matter inputs [20,21,22]. Guerra [42] reported that most of the total root length and root mass density observed for Argentine bahiagrass and Ecoturf RP was in the surface 0 to 15 cm. Rhizodeposits and root and rhizome litter provide soil carbon and nutrient resources for soil microbes, thereby likely creating niche differentiation for soil bacteria [20,53].
We found that members of the phylum Proteobacteria were enriched in the 0 to 15 cm. Interestingly, most of the bacterial genera were potential N2 fixers, including members of Bradyrhizobium, Skermanella, and Mesorhizobium. The concentration of N2-fixing bacteria in the surface soil, compared to 15 to 30 cm depth, might be related to a greater presence of roots/nodules and soil C [54,55]. Bacterial genera differentially selected at the 15 to 30 cm soil depth were a mixture of phyla with diverse putative functions, such as N2 fixation and P solubilization (Paenibacillus; Firmicutes) [56], denitrification (Rhodanobacter; Proteobacteria) [57], biodegradation (Fictibacillus; Firmicutes) [58], and release of plant beneficial metabolites (Nonomuraea; Actinobacteriota) [59]. This diversity in bacterial genera and their functions highlights the complex and dynamic interactions within bahiagrass–RP mixture, emphasizing the need for further research to unravel soil bacteria roles and contributions in pasture production.

5. Conclusions

In this study, we found increases in soil bacterial diversity and shifts in community composition in response to the long-term integration of RP into the bahiagrass system. There was a shift in previously reported Proteobacteria-dominated early establishment systems to a Firmicute-dominated system in our study. Substantial microbial differentiation was observed between 0 to 15 and 15 to 30 cm, with N2-fixing bacteria belonging to the phylum Proteobacteria dominating surface soil (0 to 15 cm depth). A mixture of bacterial phyla with diverse plant-promoting abilities was enriched in the 15 to 30 cm soil. Well-established (several years) RP in bahiagrass pastures increases soil bacterial diversity and enhance soil microbial taxa that have the potential to improve plant litter decomposition and suppress plant pathogens present in soil. However, further study is needed to better understand the roles of these soil bacterial taxa in maintaining efficient and healthy pastures. Our findings demonstrate the importance establishment time for perennial forages has in shaping soil microbial community composition. This study, however, only reports data from one time-point; there is a need to conduct more studies across different time points and to consider more long-term studies to understand the interactions within soil microbial communities in perennial forage systems.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms11123002/s1, Figure S1: Principle coordinates analysis; Figure S2: A heatmap showing core microbiome; Figure S3: The effect of (A) forage treatment, (B) soil depth, and (C) forage treatment x soil depth on the relative abundance of significant core microbiome; Table S1: ANOVA table showing the effect of forage treatment (FT), soil depth (D), and their interactions (FT × D) on the relative abundance of core soil microbiome at the genus level.

Author Contributions

Conceptualization, A.S.E., V.A.G., J.-C.L., C.L.M., A.R.S.B., J.C.B.D.J. and H.-L.L.; methodology, A.S.E., V.A.G., J.-C.L., C.L.M. and H.-L.L.; software, A.S.E.; validation, A.S.E. and H.-L.L.; formal analysis, A.S.E.; investigation, A.S.E.; resources, H.-L.L.; data curation, A.S.E.; writing—original draft preparation, A.S.E.; writing—review and editing, A.S.E., V.A.G., C.L.M., A.R.S.B., J.C.B.D.J. and H.-L.L.; visualization, A.S.E.; supervision, H.-L.L.; project administration, H.-L.L.; funding acquisition, A.S.E., C.L.M., A.R.S.B., J.C.B.D.J. and H.-L.L. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by the Soil, Water, and Ecosystem Sciences Graduate School Matching Funding to Adesuwa Erhunmwunse, National Institute of Food and Agriculture, U.S. Department of Agriculture, [grant number 2021-38640-34724] through the Southern Sustainable Agriculture Research and Education program [subaward number GS22-268] to Hui-Ling Liao and Adesuwa Erhunmwunse, and a United States Department of Agriculture-National Institute of Food and Agriculture grant [grant number 2019-67013-29107] to Hui-Ling Liao.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The raw reads used in this study can be found at online repository (http://trace.ncbi.nlm.nih.gov/Traces/sra/ under bioproject accession number, PRJNA835799, with submission ID for bacteria (SUB13901473).

Acknowledgments

We appreciate Cheryl Mackowiak’s team for establishing and maintaining the field site.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Macharia, P.N.; Kinyamario, J.I.; Ekaya, W.N.; Gachene, C.K.K.; Mureithi, J.G.; Thuranira, E.G. Evaluation of Forage Legumes for Introduction into Natural Pastures of Semi-Arid Rangelands of Kenya. Grass Forage Sci. 2010, 65, 456–462. [Google Scholar] [CrossRef]
  2. Villegas, D.M.; Velasquez, J.; Arango, J.; Obregon, K.; Rao, I.M.; Rosas, G.; Oberson, A. Urochloa Grasses Swap Nitrogen Source When Grown in Association with Legumes in Tropical Pastures. Diversity 2020, 12, 419. [Google Scholar] [CrossRef]
  3. Sollenberger, L.E.; Dubeux Junior, J.C.B. Warm-Climate, Legume-Grass Forage Mixtures versus Grass-Only Swards: An Ecosystem Services Comparison. Rev. Bras. De Zootec. 2022, 51, e20210198. [Google Scholar] [CrossRef]
  4. Ortega-S, J.A.; Sollenberger, L.E.; Quesenberry, K.H.; Jones, C.S., Jr.; Cornell, J.A. Productivity and Persistence of Rhizoma Peanut Pastures under Different Grazing Managements. Agron. J. 1992, 84, 799–804. [Google Scholar] [CrossRef]
  5. Williams, M.J.; Hammond, A.C.; Kunkle, W.E.; Spreen, T.H. Stocker Performance on Continuously Grazed Mixed Grass-Rhizoma Peanut and Bahiagrass Pastures. J. Prod. Agric. 1991, 4, 19–24. [Google Scholar] [CrossRef]
  6. Santos, E.R.S.; Dubeux, J.C.B., Jr.; Sollenberger, L.E.; Blount, A.R.S.; Mackowiak, C.; DiLorenzo, N.; Jaramillo, D.M.; Garcia, L.; Pereira, T.P.; Ruiz-Moreno, M. Herbage Responses and Biological N2 Fixation of Bahiagrass and Rhizoma Peanut Monocultures Compared with Their Binary Mixtures. Crop Sci. 2018, 58, 2149–2163. [Google Scholar] [CrossRef]
  7. Jaramillo, D.M.; Dubeux, J.C.B.; Sollenberger, L.E.; Vendramini, J.M.B.; Mackowiak, C.; DiLorenzo, N.; Garcia, L.; Queiroz, L.M.D.; Santos, E.R.S.; Homem, B.G.C.; et al. Water Footprint, Herbage, and Livestock Responses for Nitrogen-fertilized Grass and Grass–legume Grazing Systems. Crop Sci. 2021, 61, 3844–3858. [Google Scholar] [CrossRef]
  8. Kohmann, M.M.; de Oliveira Bauer, M.; Sollenberger, L.E.; Moreno, L.S.B.; da Silva, L.S.; Saraiva, S.; Dubeux, J.C.B. Legume Proportion Affects Bahiagrass–rhizoma Peanut Mixture Production and Nutritive Value and Legume Composition of Cattle Diets. Appl. Anim. Sci. 2022, 38, 560–569. [Google Scholar] [CrossRef]
  9. Wang, X.-B.; Hsu, C.-M.; Dubeux, J.C.B., Jr.; Mackowiak, C.; Blount, A.; Han, X.-G.; Liao, H.-L. Effects of Rhizoma Peanut Cultivars (Arachis glabrata Benth.) on the Soil Bacterial Diversity and Predicted Function in Nitrogen Fixation. Ecol. Evol. 2019, 9, 12676–12687. [Google Scholar] [CrossRef]
  10. Guerra, V.A.; Beule, L.; Mackowiak, C.L.; Dubeux, J.C.B., Jr.; Blount, A.R.S.; Wang, X.-B.; Rowland, D.L.; Liao, H.-L. Soil Bacterial Community Response to Rhizoma Peanut Incorporation into Florida Pastures. J. Environ. Qual. 2022, 51, 55–65. [Google Scholar] [CrossRef]
  11. Erhunmwunse, A.S.; Queiroz, L.M.D.; Zhang, K.; Mackowiak, C.L.; Blount, A.R.S.; Dubeux, J.C.B.; Liao, H.-L. Changes in Soil Microbial Diversity and Community Composition across Bahiagrass and Rhizoma Peanut Pastures. Biol. Fertil. Soils 2023, 59, 285–300. [Google Scholar] [CrossRef]
  12. Erhunmwunse, A.S.; Mackowiak, C.L.; Blount, A.R.S.; Dubeux, J.C.B.; Ogram, A.; Liao, H.-L. Short-Term Perennial Peanut Integration into Bahiagrass System Influence on Soil Microbial-Mediated Nitrogen Cycling Activities and Microbial Co-Occurrence Networks. Eur. J. Soil Biol. 2023, 119, 103566. [Google Scholar] [CrossRef]
  13. Wardle, D.A.; Bardgett, R.D.; Klironomos, J.N.; Setälä, H.; van der Putten, W.H.; Wall, D.H. Ecological Linkages between Aboveground and Belowground Biota. Science 2004, 304, 1629–1633. [Google Scholar] [CrossRef] [PubMed]
  14. Kardol, P.; De Deyn, G.B.; Laliberté, E.; Mariotte, P.; Hawkes, C.V. Biotic Plant-Soil Feedbacks across Temporal Scales. J. Ecol. 2013, 101, 309–315. [Google Scholar] [CrossRef]
  15. Schmid, M.W.; van Moorsel, S.J.; Hahl, T.; De Luca, E.; De Deyn, G.B.; Wagg, C.; Niklaus, P.A.; Schmid, B. Effects of Plant Community History, Soil Legacy and Plant Diversity on Soil Microbial Communities. J. Ecol. 2021, 109, 3007–3023. [Google Scholar] [CrossRef]
  16. Kulmatiski, A.; Beard, K.H. Long-Term Plant Growth Legacies Overwhelm Short-Term Plant Growth Effects on Soil Microbial Community Structure. Soil Biol. Biochem. 2011, 43, 823–830. [Google Scholar] [CrossRef]
  17. Hannula, S.E.; Heinen, R.; Huberty, M.; Steinauer, K.; De Long, J.R.; Jongen, R.; Bezemer, T.M. Persistence of Plant-Mediated Microbial Soil Legacy Effects in Soil and inside Roots. Nat. Commun. 2021, 12, 5686. [Google Scholar] [CrossRef]
  18. Williams, M.J.; Kelly-Begazo, C.A.; Stanley, R.L., Jr.; Quesenberry, K.H.; Prine, G.M. Establishment of Rhizoma Peanut: Interaction of Cultivar, Planting Date, and Location on Emergence and Rate of Cover. Agron. J. 1997, 89, 981–987. [Google Scholar] [CrossRef]
  19. Castillo, M.S.; Sollenberger, L.E.; Blount, A.R.; Ferrell, J.A.; Williams, M.J.; Mackowiak, C.L. Strip Planting a Legume into Warm-season Grass Pasture: Defoliation Effects during the Year of Establishment. Crop Sci. 2013, 53, 724–731. [Google Scholar] [CrossRef]
  20. Fierer, N.; Schimel, J.P.; Holden, P.A. Variations in Microbial Community Composition through Two Soil Depth Profiles. Soil Biol. Biochem. 2003, 35, 167–176. [Google Scholar] [CrossRef]
  21. Allison, V.J.; Yermakov, Z.; Miller, R.M.; Jastrow, J.D.; Matamala, R. Using Landscape and Depth Gradients to Decouple the Impact of Correlated Environmental Variables on Soil Microbial Community Composition. Soil Biol. Biochem. 2007, 39, 505–516. [Google Scholar] [CrossRef]
  22. Hao, J.; Chai, Y.N.; Lopes, L.D.; Ordóñez, R.A.; Wright, E.E.; Archontoulis, S.; Schachtman, D.P. The Effects of Soil Depth on the Structure of Microbial Communities in Agricultural Soils in Iowa (United States). Appl. Environ. Microbiol. 2021, 87, e02673-20. [Google Scholar] [CrossRef] [PubMed]
  23. van der Heijden, M.G.A.; Bardgett, R.D.; van Straalen, N.M. The Unseen Majority: Soil Microbes as Drivers of Plant Diversity and Productivity in Terrestrial Ecosystems. Ecol. Lett. 2008, 11, 296–310. [Google Scholar] [CrossRef] [PubMed]
  24. Maron, P.-A.; Sarr, A.; Kaisermann, A.; Lévêque, J.; Mathieu, O.; Guigue, J.; Karimi, B.; Bernard, L.; Dequiedt, S.; Terrat, S.; et al. High Microbial Diversity Promotes Soil Ecosystem Functioning. Appl. Environ. Microbiol. 2018, 84, e02738-17. [Google Scholar] [CrossRef] [PubMed]
  25. Soil Survey Staff, Natural Resources Conservation Service, United States Department of Agriculture. Web Soil Survey. 2007. Available online: https://websoilsurvey.sc.egov.usda.gov/ (accessed on 10 May 2020).
  26. Florida Automated Weather Network (FAWN). 2020. Available online: https://fawn.ifas.ufl.edu/about.php (accessed on 30 October 2023).
  27. Santos, E.R.S.; Dubeux, J.C.B., Jr.; Menezes, R.C.; Mackowiak, C.L.; Sollenberger, L.E.; Ruiz-Moreno, M.; Jaramillo, D.M.; Garcia, L.; Queiroz, L.M.D. Particulate Soil Organic Matter in Bahiagrass-Rhizoma Peanut Mixtures and Their Monocultures. Soil Sci. Soc. Am. J. 2019, 83, 658–665. [Google Scholar] [CrossRef]
  28. Takahashi, S.; Tomita, J.; Nishioka, K.; Hisada, T.; Nishijima, M. Development of a Prokaryotic Universal Primer for Simultaneous Analysis of Bacteria and Archaea Using next-Generation Sequencing. PLoS ONE 2014, 9, e105592. [Google Scholar] [CrossRef] [PubMed]
  29. Chen, K.-H.; Longley, R.; Bonito, G.; Liao, H.-L. A Two-Step PCR Protocol Enabling Flexible Primer Choice and High Sequencing Yield for Illumina MiSeq Meta-Barcoding. Agronomy 2021, 11, 1274. [Google Scholar] [CrossRef]
  30. Bolyen, E.; Rideout, J.R.; Dillon, M.R.; Bokulich, N.A.; Abnet, C.C.; Al-Ghalith, G.A.; Alexander, H.; Alm, E.J.; Arumugam, M.; Asnicar, F.; et al. Author Correction: Reproducible, Interactive, Scalable and Extensible Microbiome Data Science Using QIIME 2. Nat. Biotechnol. 2019, 37, 1091. [Google Scholar] [CrossRef]
  31. R Core Team. R: A Language and Environment for Statistical Computing; R Foundation for Statistical Computing: Vienna, Austria, 2022. [Google Scholar]
  32. Anderson, M.J. Distance-Based Tests for Homogeneity of Multivariate Dispersions. Biometrics 2006, 62, 245–253. [Google Scholar] [CrossRef]
  33. Oksanen, J.; Blanchet, F.G.; Friendly, M.; Kindt, R. Vegan: Ecological Diversity R Package, Version 2.5-4. Available online: https://cran.r-project.org/web/packages/vegan/vignettes/diversity-vegan.pdf (accessed on 6 June 2023).
  34. Anderson, M.J. A New Method for Non-parametric Multivariate Analysis of Variance. Austral Ecol. 2001, 26, 32–46. [Google Scholar] [CrossRef]
  35. Millard, P.; Singh, B.K. Does Grassland Vegetation Drive Soil Microbial Diversity? Nutr. Cycl. Agroecosyst. 2010, 88, 147–158. [Google Scholar] [CrossRef]
  36. Zhou, Y.; Zhu, H.; Fu, S.; Yao, Q. Variation in Soil Microbial Community Structure Associated with Different Legume Species Is Greater than That Associated with Different Grass Species. Front. Microbiol. 2017, 8, 1007. [Google Scholar] [CrossRef]
  37. Philippot, L.; Spor, A.; Hénault, C.; Bru, D.; Bizouard, F.; Jones, C.M.; Sarr, A.; Maron, P.-A. Loss in Microbial Diversity Affects Nitrogen Cycling in Soil. ISME J. 2013, 7, 1609–1619. [Google Scholar] [CrossRef] [PubMed]
  38. Girvan, M.S.; Campbell, C.D.; Killham, K.; Prosser, J.I.; Glover, L.A. Bacterial Diversity Promotes Community Stability and Functional Resilience after Perturbation. Environ. Microbiol. 2005, 7, 301–313. [Google Scholar] [CrossRef] [PubMed]
  39. Tardy, V.; Mathieu, O.; Lévêque, J.; Terrat, S.; Chabbi, A.; Lemanceau, P.; Ranjard, L.; Maron, P.-A. Stability of Soil Microbial Structure and Activity Depends on Microbial Diversity. Environ. Microbiol. Rep. 2014, 6, 173–183. [Google Scholar] [CrossRef] [PubMed]
  40. Lange, M.; Habekost, M.; Eisenhauer, N.; Roscher, C.; Bessler, H.; Engels, C.; Oelmann, Y.; Scheu, S.; Wilcke, W.; Schulze, E.-D.; et al. Biotic and Abiotic Properties Mediating Plant Diversity Effects on Soil Microbial Communities in an Experimental Grassland. PLoS ONE 2014, 9, e96182. [Google Scholar] [CrossRef] [PubMed]
  41. Dassen, S.; Cortois, R.; Martens, H.; de Hollander, M.; Kowalchuk, G.A.; van der Putten, W.H.; De Deyn, G.B. Differential Responses of Soil Bacteria, Fungi, Archaea and Protists to Plant Species Richness and Plant Functional Group Identity. Mol. Ecol. 2017, 26, 4085–4098. [Google Scholar] [CrossRef]
  42. Guerra, V.A. Soil Microbial and Ecophysiological Aspects of Rhizoma Peanut Inclusion in Bahiagrass Pastures. Ph.D. Dissertation, University of Florida, Gainesville, FL, USA, 2021. [Google Scholar]
  43. Santos, E.R.S.; Dubeux, J.C.B., Jr.; Mackowiak, C.; Blount, A.; Sollenberger, L.E.; DiLorenzo, N.; Jaramillo, D.; Garcia, L.; Pereira-Neto, J.D. Root-rhizome Mass and Chemical Composition of Bahiagrass and Rhizoma Peanut Monocultures Compared with Their Binary Mixtures. Crop Sci. 2018, 58, 955–963. [Google Scholar] [CrossRef]
  44. Daraz, U.; Erhunmwunse, A.S.; Dubeux, J.C.B., Jr.; Mackowiak, C.; Guerra, V.A.; Hsu, C.-M.; Ma, J.; Li, Y.; Yang, X.; Liao, H.-L.; et al. Soil Bacterial Communities Across Seven Rhizoma Peanut Cultivars (Arachis glabrata Benth.) Respond to Seasonal Variation. Microb. Ecol. 2023, 86, 2703–2715. [Google Scholar] [CrossRef]
  45. Peerawat, M.; Blaud, A.; Trap, J.; Chevallier, T.; Alonso, P.; Gay, F.; Thaler, P.; Spor, A.; Sebag, D.; Choosai, C.; et al. Rubber Plantation Ageing Controls Soil Biodiversity after Land Conversion from Cassava. Agric. Ecosyst. Environ. 2018, 257, 92–102. [Google Scholar] [CrossRef]
  46. Gonzalez-Pimentel, J.L.; Dominguez-Moñino, I.; Jurado, V.; Laiz, L.; Caldeira, A.T.; Saiz-Jimenez, C. The Rare Actinobacterium Crossiella Sp. Is a Potential Source of New Bioactive Compounds with Activity against Bacteria and Fungi. Microorganisms 2022, 10, 1575. [Google Scholar] [CrossRef] [PubMed]
  47. Ren, H.; Guo, H.; Shafiqul Islam, M.; Zaki, H.E.M.; Wang, Z.; Wang, H.; Qi, X.; Guo, J.; Sun, L.; Wang, Q.; et al. Improvement Effect of Biochar on Soil Microbial Community Structure and Metabolites of Decline Disease Bayberry. Front. Microbiol. 2023, 14, 1154886. [Google Scholar] [CrossRef] [PubMed]
  48. Hellequin, E.; Binet, F.; Klarzynski, O.; Hallin, S.; Juhanson, J.; Daburon, V.; Monard, C. Shaping of Soil Microbial Communities by Plants Does Not Translate into Specific Legacy Effects on Organic Carbon Mineralization. Soil Biol. Biochem. 2021, 163, 108449. [Google Scholar] [CrossRef]
  49. Warnick, T.A.; Methé, B.A.; Leschine, S.B. Clostridium Phytofermentans Sp. Nov., a Cellulolytic Mesophile from Forest Soil. Int. J. Syst. Evol. Microbiol. 2002, 52, 1155–1160. [Google Scholar] [CrossRef] [PubMed]
  50. Kadyan, S.; Panghal, M.; Kumar, S.; Singh, K.; Yadav, J.P. Assessment of Functional and Genetic Diversity of Aerobic Endospore Forming Bacilli from Rhizospheric Soil of Phyllanthus amarus L. World J. Microbiol. Biotechnol. 2013, 29, 1597–1610. [Google Scholar] [CrossRef] [PubMed]
  51. Sharma, M.; Mishra, V.; Rau, N.; Sharma, R.S. Increased Iron-Stress Resilience of Maize through Inoculation of Siderophore-Producing Arthrobacter Globiformis from Mine. J. Basic Microbiol. 2016, 56, 719–735. [Google Scholar] [CrossRef]
  52. Lugo, M.A.; Ferrero, M.; Menoyo, E.; Estévez, M.C.; Siñeriz, F.; Anton, A. Arbuscular Mycorrhizal Fungi and Rhizospheric Bacteria Diversity along an Altitudinal Gradient in South American Puna Grassland. Microb. Ecol. 2008, 55, 705–713. [Google Scholar] [CrossRef]
  53. Eilers, K.G.; Debenport, S.; Anderson, S.; Fierer, N. Digging Deeper to Find Unique Microbial Communities: The Strong Effect of Depth on the Structure of Bacterial and Archaeal Communities in Soil. Soil Biol. Biochem. 2012, 50, 58–65. [Google Scholar] [CrossRef]
  54. Rupela, O.P.; Toomsan, B.; Mittal, S.; Dart, P.J.; Thompson, J.A. Chickpea Rhizobium Populations: Survey of Influence of Season, Soil Depth and Cropping Pattern. Soil Biol. Biochem. 1987, 19, 247–252. [Google Scholar] [CrossRef]
  55. Wang, H.; Li, X.; Li, X.; Li, X.; Wang, J.; Zhang, H. Changes of Microbial Population and N-Cycling Function Genes with Depth in Three Chinese Paddy Soils. PLoS ONE 2017, 12, e0189506. [Google Scholar] [CrossRef]
  56. Grady, E.N.; MacDonald, J.; Liu, L.; Richman, A.; Yuan, Z.-C. Current Knowledge and Perspectives of Paenibacillus: A Review. Microb. Cell Fact. 2016, 15, 203. [Google Scholar] [CrossRef]
  57. Kostka, J.E.; Green, S.J.; Rishishwar, L.; Prakash, O.; Katz, L.S.; Mariño-Ramírez, L.; Jordan, I.K.; Munk, C.; Ivanova, N.; Mikhailova, N.; et al. Genome Sequences for Six Rhodanobacter Strains, Isolated from Soils and the Terrestrial Subsurface, with Variable Denitrification Capabilities. J. Bacteriol. 2012, 194, 4461–4462. [Google Scholar] [CrossRef]
  58. Chen, Y.; Wang, W.; Zhou, D.; Jing, T.; Li, K.; Zhao, Y.; Tang, W.; Qi, D.; Zhang, M.; Zang, X.; et al. Biodegradation of Lignocellulosic Agricultural Residues by a Newly Isolated Fictibacillus Sp. YS-26 Improving Carbon Metabolic Properties and Functional Diversity of the Rhizosphere Microbial Community. Bioresour. Technol. 2020, 310, 123381. [Google Scholar] [CrossRef]
  59. Sungthong, R.; Nakaew, N. The Genus Nonomuraea: A Review of a Rare Actinomycete Taxon for Novel Metabolites. J. Basic Microbiol. 2015, 55, 554–565. [Google Scholar] [CrossRef]
Microorganisms 11 03002 g001
Figure 1. Relative abundance (>1%) of major bacterial phyla under three forage treatments (Arg, Arg-Eco, and Eco) at two soil depths (0 to 15 and 15 to 30 cm).
Figure 1. Relative abundance (>1%) of major bacterial phyla under three forage treatments (Arg, Arg-Eco, and Eco) at two soil depths (0 to 15 and 15 to 30 cm).
Microorganisms 11 03002 g001
Microorganisms 11 03002 g002
Figure 2. Differential abundant bacterial genera among (A) forage treatments and (B) soil depths based on Lefse analysis. Differences in abundance among forage species (A) and soil depth (B) are coded by color (see legends). The linear discriminant analysis effect size (LEfSe) of microbial communities with LDA scores greater than 2.0 was considered.
Figure 2. Differential abundant bacterial genera among (A) forage treatments and (B) soil depths based on Lefse analysis. Differences in abundance among forage species (A) and soil depth (B) are coded by color (see legends). The linear discriminant analysis effect size (LEfSe) of microbial communities with LDA scores greater than 2.0 was considered.
Microorganisms 11 03002 g002
Table 1. Bacterial alpha diversity indices (observed features and Shannon diversity) under three forage treatments and two soil depths.
Table 1. Bacterial alpha diversity indices (observed features and Shannon diversity) under three forage treatments and two soil depths.
Observed FeaturesShannon Diversity
Forage treatment (FT)
Arg353 c7.3 c
Arg-Eco481 b7.8 b
Eco508 a8.1 a
Soil depth (D)
0 to 15 486 A8.0 A
15 to 30 408 B7.5 B
Source of variationp values 1
FT0.024 *0.046 *
D0.047 *0.049 *
FT × D0.4390.643
1 p values from ANOVA table showing the effect of forage treatment, soil depth, and their interactions on bacteria alpha diversity. * indicate significance at p < 0.05. Pairwise comparisons were conducted using Tukey’s analysis. Lowercase letters show significant differences among forage treatments and uppercase letters show significant differences between soil depths.
Table 2. PERMANOVA partitioning and analysis of forage treatment (FT), soil depth (D), and their interactions (FT × D) based on Bray–Curtis dissimilarities of amplicon sequence variant counts.
Table 2. PERMANOVA partitioning and analysis of forage treatment (FT), soil depth (D), and their interactions (FT × D) based on Bray–Curtis dissimilarities of amplicon sequence variant counts.
Source of VariationDfMSPseudo FR2p
FT20.2711.8220.140.024 *
D11.0977.3620.28<0.001 ***
FT × D20.2441.4020.120.047 *
* and *** show significance at p < 0.05 and <0.0001, respectively.
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Erhunmwunse, A.S.; Guerra, V.A.; Liu, J.-C.; Mackowiak, C.L.; Blount, A.R.S.; Dubeux, J.C.B., Jr.; Liao, H.-L. Soil Bacterial Diversity Responds to Long-Term Establishment of Perennial Legumes in Warm-Season Grassland at Two Soil Depths. Microorganisms 2023, 11, 3002. https://doi.org/10.3390/microorganisms11123002

AMA Style

Erhunmwunse AS, Guerra VA, Liu J-C, Mackowiak CL, Blount ARS, Dubeux JCB Jr., Liao H-L. Soil Bacterial Diversity Responds to Long-Term Establishment of Perennial Legumes in Warm-Season Grassland at Two Soil Depths. Microorganisms. 2023; 11(12):3002. https://doi.org/10.3390/microorganisms11123002

Chicago/Turabian Style

Erhunmwunse, Adesuwa Sylvia, Victor Alonso Guerra, Jung-Chen Liu, Cheryl L. Mackowiak, Ann Rachel Soffes Blount, José Carlos Batista Dubeux, Jr., and Hui-Ling Liao. 2023. "Soil Bacterial Diversity Responds to Long-Term Establishment of Perennial Legumes in Warm-Season Grassland at Two Soil Depths" Microorganisms 11, no. 12: 3002. https://doi.org/10.3390/microorganisms11123002

APA Style

Erhunmwunse, A. S., Guerra, V. A., Liu, J.-C., Mackowiak, C. L., Blount, A. R. S., Dubeux, J. C. B., Jr., & Liao, H.-L. (2023). Soil Bacterial Diversity Responds to Long-Term Establishment of Perennial Legumes in Warm-Season Grassland at Two Soil Depths. Microorganisms, 11(12), 3002. https://doi.org/10.3390/microorganisms11123002

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop
  NODES
admin 3
Association 5
COMMUNITY 34
Idea 2
idea 2
innovation 2
INTERN 31
Note 9
Project 4
twitter 1