Wikipedia

Eva Nogales

Eva Nogales (born in Colmenar Viejo, Madrid, Spain) is a Spanish-American biophysicist at the Lawrence Berkeley National Laboratory and a professor at the University of California, Berkeley, where she served as head of the Division of Biochemistry, Biophysics and Structural Biology of the Department of Molecular and Cell Biology (2015–2020). She is a Howard Hughes Medical Institute investigator.

Eva Nogales
Nogales in 2023
BornMay 16, 1965 (1965-05-16) (age 59)
Colmenar Viejo, Madrid, Spain
Alma materB.S., physics, Autonomous University of Madrid in 1988, PhD, University of Keele, 1992
Occupation(s)Biophysicist, professor
Known forThe first to determine the atomic structure of tubulin by electron crystallography
SpouseHoward Padmore
Children2
AwardsEarly Career Award, American Society for Cell Biology (2005)
Chabot Science Award for Excellence (2006)
Shaw Prize (2023)
Scientific career
InstitutionsUniversity of California, Berkeley, Howard Hughes Medical Institute, Synchrotron Radiation Source

Nogales is a pioneer in using electron microscopy for the structural and functional characterization of macromolecular complexes. She used electron crystallography to obtain the first structure of tubulin and identify the binding site of the important anti-cancer drug taxol. She is a leader in combining cryo-EM, computational image analysis and biochemical assays to gain insights into function and regulation of biological complexes and molecular machines.[1] Her work has uncovered aspects of cellular function that are relevant to the treatment of cancer and other diseases.[2]

Early life and education

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Eva Nogales obtained her BS degree in physics from the Autonomous University of Madrid in 1988.[3] She later earned her PhD from the University of Keele in 1992 while working at the Synchrotron Radiation Source under the supervision of Joan Bordas.[3]

Career

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During her post-doctoral work in the laboratory of Ken Downing at the Lawrence Berkeley National Laboratory, Eva Nogales was the first to determine the atomic structure of tubulin and the location of the taxol-binding site by electron crystallography.[4][5][6] She became an assistant professor in the Department of Molecular and Cell Biology at the University of California, Berkeley in 1998. In 2000 she became an investigator in the Howard Hughes Medical Institute. As cryo-EM techniques became more powerful,[7] she became a leader in applying cryo-EM to the study of microtubule structure and function[8] and other large macromolecular assemblies such as eukaryotic transcription and translation initiation complexes,[9][10] the polycomb complex PRC2, and telomerase.[11]

Selected publications

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  • Nogales, Eva; Whittaker, Michael; Milligan, Ronald A.; Downing, Kenneth H. (January 1999). "High-Resolution Model of the Microtubule". Cell. 96 (1): 79–88. doi:10.1016/s0092-8674(00)80961-7. ISSN 0092-8674. PMID 9989499.
  • Löwe, J; Li, H; Downing, K. H; Nogales, E (9 November 2001). "Refined structure of αβ-tubulin at 3.5 Å resolution11Edited by I. A. Wilson". Journal of Molecular Biology. 313 (5): 1045–1057. doi:10.1006/jmbi.2001.5077. ISSN 0022-2836. PMID 11700061.
  • Nogales, Eva (1 June 2001). "Structural Insights into Microtubule Function". Annual Review of Biophysics and Biomolecular Structure. 30 (1): 397–420. doi:10.1146/annurev.biophys.30.1.397. ISSN 1056-8700. PMID 11441808.

Awards

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Personal life

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Nogales is married to Howard Padmore and they have two children.[20]

References

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  1. ^ "How to See Living Machines". Medical Design Technology. 2016-12-07. Retrieved 2018-02-03.
  2. ^ "Eva Nogales". HHMI.org. Retrieved 2019-03-23.
  3. ^ a b "Nogales Lab – CryoEM – Publications". cryoem.berkeley.edu. Archived from the original on 2021-12-25. Retrieved 2022-03-16.
  4. ^ Nogales, E.; Wolf, S. G.; Khan, I. A.; Ludueña, R. F.; Downing, K. H. (1995-06-01). "Structure of tubulin at 6.5 A and location of the taxol-binding site". Nature. 375 (6530): 424–427. Bibcode:1995Natur.375..424N. doi:10.1038/375424a0. ISSN 0028-0836. PMID 7760939. S2CID 4338992.
  5. ^ Nogales, E., Wolf, S. G. and Downing, K. H. (1998.) Structure of the ab tubulin dimer by electron crystallography. Nature, 391, 199-203.
  6. ^ Nogales, E.; Whittaker, M.; Milligan, R. A.; Downing, K. H. (1999-01-08). "High-resolution model of the microtubule". Cell. 96 (1): 79–88. doi:10.1016/s0092-8674(00)80961-7. ISSN 0092-8674. PMID 9989499. S2CID 18422680.
  7. ^ Callaway, Ewen (2015-09-10). "The revolution will not be crystallized: a new method sweeps through structural biology". Nature. 525 (7568): 172–174. Bibcode:2015Natur.525..172C. doi:10.1038/525172a. ISSN 1476-4687. PMID 26354465.
  8. ^ Downing, Kenneth H.; Nogales, Eva (2010). "Cryoelectron Microscopy Applications in the Study of Tubulin Structure, Microtubule Architecture, Dynamics and Assemblies, and Interaction of Microtubules with Motors". Cryo-EM, Part C: Analyses, Interpretation, and Case studies. Methods in Enzymology. Vol. 483. pp. 121–142. doi:10.1016/S0076-6879(10)83006-X. ISBN 9780123849939. ISSN 1557-7988. PMC 4165512. PMID 20888472.
  9. ^ Michael A. Cianfrocco, George A. Kassavetis, Patricia Grob, Jie Fang, Tamar Juven-Gershon, James T. Kadonaga, Eva Nogales (2013.) Human TFIID Binds to Core Promoter DNA in a Reorganized Structural State. Cell, 152(1):120–131.
  10. ^ "Stop-Motion View of DNA-Binding Complex May Animate Drug Discovery". GEN – Genetic Engineering and Biotechnology News. 2018-11-20. Retrieved 2019-03-23.
  11. ^ "The 3D Structure of Telomerase: Uncovering Its Role in Human Disease". Drug Discovery from Technology Networks. Retrieved 2019-03-23.
  12. ^ "Early Career Life Scientist Award". ASCB. Retrieved 2019-03-23.
  13. ^ "Announcing the winners of the 2015 Protein Society Awards". EurekAlert!. Retrieved 2019-03-23.
  14. ^ "Eva Nogales". nasonline.org. 16 April 2021. Retrieved 29 December 2023.
  15. ^ "9 campus faculty selected for membership in American Academy of Arts and Sciences | The Daily Californian". The Daily Californian. 2016-04-22. Retrieved 2018-02-03.
  16. ^ "Women in Cell Biology Awards". ASCB. Retrieved 2019-03-23.
  17. ^ "Nogales Receives 2019 Grimwade Medal". Biosciences Area. 2019-03-07. Retrieved 2019-03-23.
  18. ^ "AAAS Honors Outstanding Scientific Contributors as 2021 AAAS Fellows | American Association for the Advancement of Science". www.aaas.org. Retrieved 2022-03-16.
  19. ^ Shaw Prize 2023
  20. ^ Phelan, Laura (1 December 2014). "Eva Nogales". The Biophysical Society. Retrieved 29 December 2023.
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