Ezrin

Ezrin also known as cytovillin or villin-2 is a protein that in humans is encoded by the EZR gene.

EZR
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4RM8, 4RMA, 4RM9, 1NI2
Identifiers
Aliases	EZR, CVIL, CVL, HEL-S-105, VIL2, Ezrin
External IDs	OMIM: 123900; MGI: 98931; HomoloGene: 55740; GeneCards: EZR; OMA:EZR - orthologs
Gene location (Human) Chr. Chromosome 6 (human) Band 6q25.3 Start 158,765,741 bp End 158,819,368 bp
Gene location (Mouse) Chr. Chromosome 17 (mouse) Band 17|17 A1 Start 7,005,440 bp End 7,050,183 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in ventricular zone bronchial epithelial cell ganglionic eminence nasal epithelium olfactory zone of nasal mucosa mucosa of paranasal sinus tendon of biceps brachii right adrenal cortex right uterine tube jejunal mucosa Top expressed in epithelium of stomach mucous cell of stomach left colon epithelium of small intestine intestinal villus ileum seminal vesicula Ileal epithelium pyloric antrum Epithelium of choroid plexus More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein domain specific binding protein-containing complex binding cytoskeletal protein binding protein kinase A regulatory subunit binding protein kinase A catalytic subunit binding S100 protein binding actin filament binding protein binding cell adhesion molecule binding microtubule binding ATPase binding actin binding RNA binding cadherin binding protein C-terminus binding disordered domain specific binding protein kinase A binding identical protein binding Cellular component cytoplasm cytosol endosome membrane focal adhesion T-tubule ruffle Schwann cell microvillus ruffle membrane actin cytoskeleton perinuclear region of cytoplasm cytoskeleton cell projection cytoplasmic side of apical plasma membrane myelin sheath microvillus microspike apical plasma membrane membrane raft extracellular exosome microvillus membrane ciliary basal body filopodium cortical cytoskeleton plasma membrane apical part of cell astrocyte projection intracellular anatomical structure cell cortex brush border actin filament TCR signalosome cell periphery cell body vesicle plasma membrane raft uropod basolateral plasma membrane immunological synapse cell tip fibrillar center extracellular space extrinsic component of membrane protein-containing complex Biological process leukocyte cell-cell adhesion regulation of organelle assembly sphingosine-1-phosphate receptor signaling pathway negative regulation of p38MAPK cascade actin filament bundle assembly establishment of epithelial cell apical/basal polarity positive regulation of multicellular organism growth astral microtubule organization cellular response to cAMP establishment of endothelial barrier microvillus assembly negative regulation of T cell receptor signaling pathway gland morphogenesis cortical microtubule organization positive regulation of protein localization to early endosome positive regulation of early endosome to late endosome transport intestinal D-glucose absorption establishment or maintenance of apical/basal cell polarity regulation of NIK/NF-kappaB signaling epithelial cell differentiation regulation of actin cytoskeleton organization negative regulation of transcription by RNA polymerase II positive regulation of protein secretion membrane to membrane docking regulation of cell shape protein kinase A signaling establishment of centrosome localization phosphatidylinositol-mediated signaling regulation of cell size actin cytoskeleton reorganization filopodium assembly receptor internalization terminal web assembly axon guidance positive regulation of gene expression negative regulation of ERK1 and ERK2 cascade protein localization to cell cortex regulation of microvillus length protein localization to plasma membrane positive regulation of protein localization to plasma membrane Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 7430 22350 Ensembl ENSG00000092820 ENSMUSG00000052397 UniProt P15311 P26040 RefSeq (mRNA) NM_001111077 NM_003379 NM_009510 RefSeq (protein) NP_001104547 NP_003370 NP_033536 Location (UCSC) Chr 6: 158.77 – 158.82 Mb Chr 17: 7.01 – 7.05 Mb PubMed search
Wikidata
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"Cvl" redirects here. For other uses, see CVL.

Structure

The N-terminus of ezrin contains a FERM domain which is further subdivided into three subdomains. The C-terminus contains an ERM domain.

Function

The cytoplasmic peripheral protein encoded by this gene can be phosphorylated by protein-tyrosine kinase in microvilli and is a member of the ERM protein family. This protein serves as a linker between plasma membrane and actin cytoskeleton. It plays a key role in cell surface structure adhesion, migration, and organization.

The N-terminal domain (also called FERM domain) binds sodium-hydrogen exchanger regulatory factor (NHERF) protein (involving long-range allostery). This binding can happen only when ezrin is in its active state. The activation of ezrin occurs in synergism of the two factors: 1) binding of the N-terminal domain to phosphatidylinositol(4,5)bis-phosphate (PIP2) and 2) phosphorylation of threonine T567 in the C-terminal domain. Binding to actin filaments (via C-terminal) and to membrane proteins (via N-terminal) stabilizes the protein's conformation in its active mode. The membrane proteins like CD44 and ICAM-2 are indirect binding partners of ezrin, while EBP50 (ERM binding protein 50) can associate with ezrin directly.

Interactions

VIL2 has been shown to interact with:

• CD43,
• FASLG,
• ICAM-1,
• ICAM2,
• ICAM3,
• Merlin,
• MSN,
• PIK3R1,
• PALLD
• S100P,
• SDC2,
• SLC9A3R1,
• SLC9A3R2, and
• VCAM-1.

Further reading

• Martin TA, Harrison G, Mansel RE, Jiang WG (2004). "The role of the CD44/ezrin complex in cancer metastasis". Crit. Rev. Oncol. Hematol. 46 (2): 165–86. doi:10.1016/S1040-8428(02)00172-5. PMID 12711360.