The effect of extra virgin olive oil on HSP27 expression in the cerebral cortex of male Rattus norvegicus middle cerebral artery occlusion model

Abdulloh Machin; Viskasari Pintoko Kalanjati; Anisa Nur’aini Abidah; paulus sugianto; Joni Susanto; Azizah Amimathul Firdha

doi:10.29219/fnr.v68.9500

Abdulloh Machin Departement of Neurology, Faculty of Medicine, Universitas Airlangga
Viskasari Pintoko Kalanjati Departement of Anatomy and Histology, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA
Anisa Nur’aini Abidah Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA
paulus sugianto Departement of Neurology, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA
Joni Susanto Departement of Anatomy and Histology, Faculty of Medicine, Universitas Airlangga, Surabaya, INDONESIA
Azizah Amimathul Firdha Indonesian Medical Association (IDI) Surabaya chapter, Surabaya, INDONESIA

DOI: https://doi.org/10.29219/fnr.v68.9500

Keywords: evoo, olive oil, HSP27, MCAO, stroke

Abstract

Background: Ischemic stroke produces oxidative stress in neurons and neuroglial, triggering a cellular response with HSP27 as one of the markers. Extra virgin olive oil (EVOO) is reported to act as a neuroprotection by inhibiting reactive oxygen species, which can modulate HSP27.

Objective: To analyze the potential neuroprotection of EVOO containing 7-g MUFA on the expression of HSP27 in the cerebral cortex of Rattus norvegicus Wistar strain middle cerebral artery occlusion (MCAO) model.

Design: MCAO-Rattus norvegicus Wistar male rodents (n = 32), randomly divided into negative control (sham), positive control (P0), MCAO added EVOO 0.5 mL/kg body weight (P1), and MCAO added EVOO 2 mL/kg body weight (P2); the experiment was conducted 14 days. The nuclei of neurons, glial, and expression of HSP27 on the cerebral cortex were analyzed using Image J software, data were compared between groups with a significance level of P < 0.05.

Results: There were significant differences in the neuron and glia nuclei numbers between groups (P = 0.01). There was also a significant difference in cerebral cortex HSP27 expression between groups (P = 0.006).

Conclusion: There was a significant down-regulated of HSP27 in the cerebral cortex of the male Rattus norvegicus Wistar strain MCAO model given EVOO with 7 g of MUFA content at a dose of 0.5–2 mL/kgBW.

Downloads

Download data is not yet available.

References

1.
Grysiewicz RA, Thomas K, Pandey DK. Epidemiology of ischemic and hemorrhagic stroke: incidence, prevalence, mortality, and risk factors. Neurol Clin 2008; 26(4): 871–95. doi: 10.1016/j.ncl.2008.07.003

2.
World Health Organization. Global health estimates: life expectancy and leading causes of death and disability. Genewa: World Health Organization; 2019.

3.
Deb P, Sharma S, Hassan KM. Pathophysiologic mechanisms of acute ischemic stroke: an overview with emphasis on therapeutic significance beyond thrombolysis. Pathophysiology 2010; 17(3): 197–218. doi: 10.1016/j.pathophys.2009.12.001

4.
Huang J, Upadhyay UM, Tamargo RJ. Inflammation in stroke and focal cerebral ischemia. Surg Neurol 2006; 66(3): 232–45. doi: 10.1016/j.surneu.2005.12.028

5.
Shankar K, Mehendale HM. Oxidative stress. In: Wexler P, ed. Encyclopedia of Toxicology. 3rd ed. Oxford: Academic Press; 2014, pp. 735–7.

6.
Fulda S, Gorman AM, Hori O, Samali A. Cellular stress responses: cell survival and cell death. Int J Cell Biol 2010; 2010: 1–23. doi: 10.1155/2010/214074

7.
Shimura H, Tanaka R, Urabe T, Hattori N. Heat shock protein 27 (HSP27) as a therapeutic _target in ischemic stroke and neurodegenerative disorders. Juntendo Med J 2017; 63(1): 17–21. doi: 10.14789/jmj.63.17

8.
Havasi A, Li Z, Wang Z, Martin JL, Botla V, Ruchalski K, et al. Hsp27 inhibits bax activation and apoptosis via a phosphatidylinositol 3-kinase-dependent mechanism. J Biol Chem 2008; 283(18): 12305–13. doi: 10.1074/jbc.M801291200

9.
Stetler RA, Gao Y, Zhang L, Weng Z, Zhang F, Hu X, et al. Phosphorylation of HSP27 by protein kinase D is essential for mediating neuroprotection against ischemic neuronal injury. J Neurosci 2012; 32(8): 2667–82. doi: 10.1523/JNEUROSCI.5169-11.2012

10.
Rabiei Z, Bigdeli MR, Rasoulian B, Ghassempour A, Mirzajani F. The neuroprotection effect of pretreatment with olive leaf extract on brain lipidomics in rat stroke model. Phytomedicine 2012; 19(10): 940–6. doi: 10.1016/j.phymed.2012.06.003

11.
Angeloni C, Malaguti M, Barbalace M, Hrelia S. Bioactivity of olive oil phenols in neuroprotection. IJMS 2017; 18(11): 2230. doi: 10.3390/ijms18112230

12.
Yu H, Liu P, Tang H, Jing J, Lv X, Chen L, et al. Oleuropein, a natural extract from plants, offers neuroprotection in focal cerebral ischemia/reperfusion injury in mice. Eur J Pharmacol 2016; 775: 113–19. doi: 10.1016/j.ejphar.2016.02.027

13.
Bali E, Ergin V, Rackova L, Bayraktar O, Küçükboyacı N, Karasu Ç. Olive leaf extracts protect cardiomyocytes against 4-hydroxynonenal-induced toxicity in vitro: comparison with oleuropein, hydroxytyrosol, and quercetin. Planta Med 2014; 80(12): 984–92. doi: 10.1055/s-0034-1382881

14.
Machin A, Susilo I, Purwanto DA. Green tea and its active compound epigallocathechin-3-gallate (EGCG) inhibit neuronal apoptosis in a middle cerebral artery occlusion (MCAO) model. J Basic Clin Physiol Pharmacol 2021; 32(4): 319–25. doi: 10.1515/jbcpp-2020-0454

15.
Williams SM, Bryan-Lluka LJ, Pow DV. Quantitative analysis of immunolabeling for serotonin and for glutamate transporters after administration of imipramine and citalopram. Brain Res 2005; 1042(2): 224–32. doi: 10.1016/j.brainres.2005.02.045

16.
Parkinson L, Cicerale S. The health benefiting mechanisms of virgin olive oil phenolic compounds. Molecules 2016; 21(12): 1734. doi: 10.3390/molecules21121734

17.
Guasch-Ferré M, Liu G, Li Y, Sampson L, Manson JE, Salas-Salvadó J, et al. Olive oil consumption and cardiovascular risk in U.S. adults. J Am Coll Cardiol 2020; 75(15): 1729–39. doi: 10.1016/j.jacc.2020.02.036

18.
Veterini AS, Soedarmo SM, Hasanul, Adi AC, Rachmawati H, Rehatta NM. Future views on nanonutrition for critically ill patients: the role of extra virgin olive oil nanoemulsion in sepsis enteral nutrition. Crit Care Shock 2020; 25(3): 208–20.

19.
Kato H, Kogure K, Liu X-H, Araki T, Kato K, Itoyama Y. Immunohistochemical localization of the low molecular weight stress protein HSP27 following focal cerebral ischemia in the rat. Brain Res 1995; 679(1): 1–7. doi: 10.1016/0006-8993(95)00198-Y

20.
Vidyasagar A, Wilson NA, Djamali A. Heat shock protein 27 (HSP27): biomarker of disease and therapeutic _target. Fibrogenesis Tissue Repair 2012; 5(1): 7. doi: 10.1186/1755-1536-5-7

21.
Popp A, Jaenisch N, Witte OW, Frahm C. Identification of ischemic regions in a rat model of stroke. PLoS One 2009; 4(3): e4764. doi: 10.1371/journal.pone.0004764

22.
Mehlen P, Kretz-Remy C, Briolay J, Fostan P, Mirault ME, Arrigo AP. Intracellular reactive oxygen species as apparent modulators of heat-shock protein 27 (hsp27) structural organization and phosphorylation in basal and tumour necrosis factor α -treated T47D human carcinoma cells. Biochem J 1995; 312(2): 367–75. doi: 10.1042/bj3120367

23.
Venugopal A, Sundaramoorthy K, Vellingiri B. Therapeutic potential of Hsp27 in neurological diseases. Egypt J Med Hum Genet 2019; 20(1): 21. doi: 10.1186/s43042-019-0023-4

24.
Rogalla T, Ehrnsperger M, Preville X, Kotlyarov A, Lutsch G, Ducasse C, et al. Regulation of Hsp27 oligomerization, chaperone function, and protective activity against oxidative stress/tumor necrosis factor α by phosphorylation. J Biol Chem 1999; 274(27): 18947–56. doi: 10.1074/jbc.274.27.18947

25.
Arrigo A-P. Hsp27: novel regulator of intracellular redox state. IUBMB Life 2001; 52(6): 303–7. doi: 10.1080/152165401317291156

26.
Mehlen P, Mehlen A, Guillet D, Preville X, Arrigo A-P. Tumor necrosis factor-α induces changes in the phosphorylation, cellular localization, and oligomerization of human hsp27, a stress protein that confers cellular resistance to this cytokine. J Cell Biochem 1995; 58(2): 248–59. doi: 10.1002/jcb.240580213

27.
de la Puerta R, Gutierrez VR, Hoult JRS. Inhibition of leukocyte 5-lipoxygenase by phenolics from virgin olive oil. Biochem Pharmacol 1999; 57(4): 445–9. doi: 10.1016/S0006-2952(98)00320-7

28.
Visioli F, Bellomo G, Galli C. Free radical-scavenging properties of olive oil polyphenols. Biochem Biophys Res Commun 1998; 247(1): 60–4. doi: 10.1006/bbrc.1998.8735