Abstract
Most bacterial cells carry prophage genomes either integrated into the host DNA or present as repressed plasmids. Methods are described for the induction of prophages using Mitomycin C, and for the isolation of prophage-cured bacterial cell lines.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Raya, R.R., H’bert, E.M. (2009). Isolation of Phage via Induction of Lysogens. In: Clokie, M.R., Kropinski, A.M. (eds) Bacteriophages. Methods in Molecular Biology™, vol 501. Humana Press. https://doi.org/10.1007/978-1-60327-164-6_3
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DOI: https://doi.org/10.1007/978-1-60327-164-6_3
Publisher Name: Humana Press
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Online ISBN: 978-1-60327-164-6
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