Summary
The effect was studied of a saluretic agent on the uptake of sodium-22 into the CSF and cerebral cortex of nephrectomized rats. Furosemide, 1 mg/kg, was injected into the left lateral ventricle of the brain. Sodium-22 was injected intraperitioneally and the uptake of the isotope into CSF, cerebral cortex, skeletal muscle and plasma was measured at intervals from 0.25 hour to 24 hours. During the initial 4-hour period after injection of the sodium-22, the uptake of the isotope was reduced in the furosemide-treated animals. The maximum reduction in uptake occurred at the 0.25-hour time period. The CSF Na RSA of the treated animals was 37% less than that of the control animals. After about 8 hours there was no difference in sodium-22 uptake into the CSF between the control and treated animals.
The uptake of sodium-22 into the cerebral cortex was decreased in the furosemide-treated animals. Since the brain extracellular space and the CSF appear to be in relatively free communication, the reduced brain radioactivity may be related to the decreased uptake of sodium-22 into the CSF.
It is suggested that the decrease in sodium-22 uptake into CSF and brain in the furosemide-treated animals may be caused by inhibition of active sodium transport in the choroid plexus.
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This study was supported, in part, by USPHS Grants 5-P01-NB-04553 and 5Tl-GM-153.
L.E.B. was a predoctoral trainee under USPHS Pharmacology Research Training Grant 5Tl-GM-153.
D.J.R. is the recipient of USPHS Research Career Development Program Award 1-K3-NB-7779.
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Buhrley, L.E., Reed, D.J. The effect of furosemide on sodium-22 uptake into cerebrospinal fluid and brain. Exp Brain Res 14, 503–510 (1972). https://doi.org/10.1007/BF00236592
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DOI: https://doi.org/10.1007/BF00236592