FIG. 5.
ERRα and PGC-1α regulate the mPDK4 promoter through the NR response element. (Top) Schematic showing the mutation (lowercase) of the NR response element in mPDK4.Luc.2281 (NRptmut.mPDK4.Luc.2281). (Bottom) Graph depicting the results of transient cotransfections in CV-1 cells performed with either the mPDK4.Luc.2281 reporter (wild type [WT]) or NRptmut.mPDK4.Luc.2281 (NRptmut). Asterisks indicate significant differences from WT mPDK4.Luc.2281 cotransfected with empty vector (P < 0.05). Dagger indicates a significant difference from mPDK4.Luc.2281 with ERRα (P < 0.05). Double dagger indicates a significant difference from the value obtained using NRptmut.mPDK4.Luc.2281 with empty vector (P < 0.05).