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. 1978 Apr;134(1):295–305. doi: 10.1128/jb.134.1.295-305.1978

Characterization of two types of yeast ribosomal DNA genes.

T D Petes, L M Hereford, K G Skryabin
PMCID: PMC222246  PMID: 348684

Abstract

The intragenic organization of ribosomal DNA from a diploid strain of Saccharomyces cerevisiae was analyzed by using recombinant DNA molecules constructed in vitro. Restriction analysis of the yeast ribosomal DNA with the EcoRI restriction enzyme indicated that eight restriction fragments were present in the ribosomal DNA of this strain: X' (1.87 X 10(6) daltons), A (1.77 X 10(6) daltons), B (1.48 X 10(6) daltons), C (1.22 X 10(6) daltons), D (0.39 X 10(6) daltons), E (0.36 X 10(6) daltons), F (0.22 X 10(6) daltons), and G (0.17 X 10(6) daltons). These fragments were distributed between two different types of ribosomal DNA genes, which had the restriction maps: (formula: see text) in which the underlined region shows the repeating unit. The diploid yeast strain contained approximately equal amounts of each of these two types of genes. The analysis of the recombinant DNA molecules also indicated that the yeast ribosomal genes are homogeneous and extensively clustered.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bell G. I., DeGennaro L. J., Gelfand D. H., Bishop R. J., Valenzuela P., Rutter W. J. Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs. J Biol Chem. 1977 Nov 25;252(22):8118–8125. [PubMed] [Google Scholar]
  2. Botchan M., Topp W., Sambrook J. The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell. 1976 Oct;9(2):269–287. doi: 10.1016/0092-8674(76)90118-5. [DOI] [PubMed] [Google Scholar]
  3. Clewell D. B., Helinski D. R. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970 Oct 27;9(22):4428–4440. doi: 10.1021/bi00824a026. [DOI] [PubMed] [Google Scholar]
  4. Cramer J. H., Bhargava M. M., Halvorson H. O. Isolation and characterization of DNA of Saccharomyces cerevisiae. J Mol Biol. 1972 Oct 28;71(1):11–20. doi: 10.1016/0022-2836(72)90396-8. [DOI] [PubMed] [Google Scholar]
  5. Cramer J. H., Farrelly F. W., Barnitz J. T., Rownd R. H. Construction and restriction endonuclease mapping of hybrid plasmids containing Saccharomyces cerevisiae ribosomal DNA. Mol Gen Genet. 1977 Mar 16;151(3):229–244. doi: 10.1007/BF00268786. [DOI] [PubMed] [Google Scholar]
  6. Cramer J. H., Farrelly F. W., Rownd R. H. Restriction endonuclease analysis of ribosomal DNA from Saccharomyces cerevisiae. Mol Gen Genet. 1976 Nov 17;148(3):233–241. doi: 10.1007/BF00332897. [DOI] [PubMed] [Google Scholar]
  7. Finkelstein D. B., Blamire J., Marmur J. Location of ribosomal RNA cistrons in yeast. Nat New Biol. 1972 Dec 27;240(104):279–281. doi: 10.1038/newbio240279a0. [DOI] [PubMed] [Google Scholar]
  8. Goldberg S., Oyen T., Idriss J. M., Halvorson H. O. Use of disomic strains to study the arrangement of ribosomal cistrons in saccharomyces of ribosomal cistrons in Saccharomyces cerevisiae. Mol Gen Genet. 1972;116(2):139–157. doi: 10.1007/BF00582223. [DOI] [PubMed] [Google Scholar]
  9. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kramer R. A., Cameron J. R., Davis R. W. Isolation of bacteriophage lambda containing yeast ribosomal RNA genes: screening by in situ RNA hybridization to plaques. Cell. 1976 Jun;8(2):227–232. doi: 10.1016/0092-8674(76)90006-4. [DOI] [PubMed] [Google Scholar]
  11. LEVINE M. Mutations in the temperate phage P22 and lysogeny in Salmonella. Virology. 1957 Feb;3(1):22–41. doi: 10.1016/0042-6822(57)90021-1. [DOI] [PubMed] [Google Scholar]
  12. Livingston D. M., Klein H. L. Deoxyribonucleic acid sequence organization of a yeast plasmid. J Bacteriol. 1977 Jan;129(1):472–481. doi: 10.1128/jb.129.1.472-481.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lobban P. E., Kaiser A. D. Enzymatic end-to end joining of DNA molecules. J Mol Biol. 1973 Aug 15;78(3):453–471. doi: 10.1016/0022-2836(73)90468-3. [DOI] [PubMed] [Google Scholar]
  14. Maniatis T., Kee S. G., Efstratiadis A., Kafatos F. C. Amplification and characterization of a beta-globin gene synthesized in vitro. Cell. 1976 Jun;8(2):163–182. doi: 10.1016/0092-8674(76)90001-5. [DOI] [PubMed] [Google Scholar]
  15. Maxam A. M., Tizard R., Skryabin K. G., Gilbert W. Promotor region for yeast 5S ribosomal RNA. Nature. 1977 Jun 16;267(5612):643–645. doi: 10.1038/267643a0. [DOI] [PubMed] [Google Scholar]
  16. McDonell M. W., Simon M. N., Studier F. W. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977 Feb 15;110(1):119–146. doi: 10.1016/s0022-2836(77)80102-2. [DOI] [PubMed] [Google Scholar]
  17. Meyerink J. H., Retèl J. Topographical analysis of yeast ribosomal DNA by cleavage with restriction endonucleases. II. The physical map of EcoRI fragments. Nucleic Acids Res. 1976 Oct;3(10):2697–2707. doi: 10.1093/nar/3.10.2697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nath K., Bollon A. P. Characterization of yeast ribosomal DNA fragments generated by EcoR1 restriction endonuclease. Mol Gen Genet. 1976 Aug 19;147(2):153–168. doi: 10.1007/BF00267567. [DOI] [PubMed] [Google Scholar]
  19. Nath K., Bollon A. P. Organization of the yeast ribosomal RNA gene cluster via cloning and restriction analysis. J Biol Chem. 1977 Sep 25;252(18):6562–6571. [PubMed] [Google Scholar]
  20. Petes T. D., Botstein D. Simple Mendelian inheritance of the reiterated ribosomal DNA of yeast. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5091–5095. doi: 10.1073/pnas.74.11.5091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rubin G. M., Sulston J. E. Physical linkage of the 5 S cistrons to the 18 S and 28 S ribosomal RNA cistrons in Saccharomyces cerevisiae. J Mol Biol. 1973 Sep 25;79(3):521–530. doi: 10.1016/0022-2836(73)90403-8. [DOI] [PubMed] [Google Scholar]
  22. Schachat F. H., Hogness D. S. Repetitive sequences in isolated Thomas circles from Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1974;38:371–381. doi: 10.1101/sqb.1974.038.01.040. [DOI] [PubMed] [Google Scholar]
  23. Schweizer E., MacKechnie C., Halvorson H. O. The redundancy of ribosomal and transfer RNA genes in Saccharomyces cerevisiae. J Mol Biol. 1969 Mar 14;40(2):261–277. doi: 10.1016/0022-2836(69)90474-4. [DOI] [PubMed] [Google Scholar]
  24. Sebring E. D., Garon C. F., Salzman N. P. Superhelix density of replicating simian virus 40 DNA molecules. J Mol Biol. 1974 Dec 5;90(2):371–379. doi: 10.1016/0022-2836(74)90380-5. [DOI] [PubMed] [Google Scholar]
  25. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  26. Skryabin K. G., Maxam A. M., Petes T. D., Hereford L. Location of the 5.8S rRNA gene of Saccharomyces cerevisiae. J Bacteriol. 1978 Apr;134(1):306–309. doi: 10.1128/jb.134.1.306-309.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  28. Stevens B. J., Moustacchi E. ADN satellite gamma et molécules circulaires torsadées de petite taille chez la levure Saccharomyces cerevisiae. Exp Cell Res. 1971 Feb;64(2):259–266. doi: 10.1016/0014-4827(71)90075-9. [DOI] [PubMed] [Google Scholar]
  29. Udem S. A., Warner J. R. Ribosomal RNA synthesis in Saccharomyces cerevisiae. J Mol Biol. 1972 Mar 28;65(2):227–242. doi: 10.1016/0022-2836(72)90279-3. [DOI] [PubMed] [Google Scholar]
  30. Williamson D. H., Fennell D. J. The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA. Methods Cell Biol. 1975;12:335–351. doi: 10.1016/s0091-679x(08)60963-2. [DOI] [PubMed] [Google Scholar]
  31. Williamson D. H., Moustacchi E. The synthesis of mitochondrial DNA during the cell cycle in the yeast Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1971 Jan 22;42(2):195–201. doi: 10.1016/0006-291x(71)90087-8. [DOI] [PubMed] [Google Scholar]

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