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. 1980 Oct;144(1):28–35. doi: 10.1128/jb.144.1.28-35.1980

Isolation and mapping of a mutation in Escherichia coli with altered levels of ribonuclease H.

P L Carl, L Bloom, R J Crouch
PMCID: PMC294580  PMID: 6998952

Abstract

A mutant of Escherichia coli with altered levels of ribonuclease (RNase) H was isolated after mutagenesis with ethyl methane sulfonate. A procedure for assaying RNase H in partially purified extracts was used to screen approximately 1,500 colonies for variations in RNase H activity. Confirmation of a lower level of RNase H in the mutant was accomplished by analysis of RNase H in sodium dodecyl sulfate-polyacrylamide gels. By Hfr, F', and P1 transduction mapping, the genetic locus responsible for the lower levels of RNase H was located at 5.1 min on the E. coli chromosome. This mutation (rnh) represents a new locus on the E. coli chromosome. The only phenotypic characteristic of this mutation which has been observed to date is the lower level of RNase H (30% of parental values).

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Selected References

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