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. 1984 Apr 11;12(7):3283–3293. doi: 10.1093/nar/12.7.3283

U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle.

C Hashimoto, J A Steitz
PMCID: PMC318745  PMID: 6201826

Abstract

U4 and U6 RNAs of mammalian cells possess extensive intermolecular sequence complementarity and hence have the potential to base pair. A U4/U6 RNA complex, detectable in nondenaturing polyacrylamide gels, is released when human small nuclear ribonucleoproteins (snRNPs) containing U1, U2, U4, U5, and U6 RNAs are dissociated with proteinase K in the presence of sodium dodecyl sulfate. The released RNA/RNA complex dissociates with increasing temperature, consistent with the existence of specific base-pairing between the two RNAs. Since U6 RNA is selectively released from intact snRNPs under the same conditions required to dissociate the U4/U6 RNA complex, the RNA-RNA interaction may be sufficient to maintain U4 and U6 RNAs in the same snRNP particle. The biological implications of these findings are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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