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. 1980 Dec 11;8(23):5779–5794. doi: 10.1093/nar/8.23.5779

The structure of the yeast ribosomal RNA genes. I. The complete nucleotide sequence of the 18S ribosomal RNA gene from Saccharomyces cerevisiae.

P M Rubtsov, M M Musakhanov, V M Zakharyev, A S Krayev, K G Skryabin, A A Bayev
PMCID: PMC324341  PMID: 7008030

Abstract

The cloned 18 S ribosomal RNA gene from Saccharomyces cerevisiae have been sequenced, using the Maxam-Gilbert procedure. From this data the complete sequence of 1789 nucleotides of the 18 S RNA was deduced. Extensive homology with many eucaryotic as well as E. coli ribosomal small subunit rRNA (S-rRNA) has been observed in the 3'-end region of the rRNA molecule. Comparison of the yeast 18 S rRNA sequences with partial sequence data, available for rRNAs of the other eucaryotes provides strong evidence that a substantial portion of the 18 S RNA sequence has been conserved in evolution.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bell G. I., DeGennaro L. J., Gelfand D. H., Bishop R. J., Valenzuela P., Rutter W. J. Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs. J Biol Chem. 1977 Nov 25;252(22):8118–8125. [PubMed] [Google Scholar]
  2. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801–4805. doi: 10.1073/pnas.75.10.4801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carbon P., Ehresmann C., Ehresmann B., Ebel J. P. The sequence of Escherichia coli ribosomal 16 S RNA determined by new rapid gel methods. FEBS Lett. 1978 Oct 1;94(1):152–156. doi: 10.1016/0014-5793(78)80926-0. [DOI] [PubMed] [Google Scholar]
  4. Choi Y. C., Busch H. Modified nucleotides in T1 RNase oligonucleotides of 18S ribosomal RNA of the Novikoff hepatoma. Biochemistry. 1978 Jun 27;17(13):2551–2560. doi: 10.1021/bi00606a015. [DOI] [PubMed] [Google Scholar]
  5. Cramer J. H., Farrelly F. W., Barnitz J. T., Rownd R. H. Construction and restriction endonuclease mapping of hybrid plasmids containing Saccharomyces cerevisiae ribosomal DNA. Mol Gen Genet. 1977 Mar 16;151(3):229–244. doi: 10.1007/BF00268786. [DOI] [PubMed] [Google Scholar]
  6. Dawid I. B., Wellauer P. K., Long E. O. Ribosomal DNA in Drosophila melanogaster. I. Isolation and characterization of cloned fragments. J Mol Biol. 1978 Dec 25;126(4):749–768. doi: 10.1016/0022-2836(78)90018-9. [DOI] [PubMed] [Google Scholar]
  7. De Jonge P., Klootwijk J., Planta R. J. Terminal nucleotide sequences of 17-S ribosomal RNA and its immediate precursor 18-S RNA in yeast. Eur J Biochem. 1977 Jan;72(2):361–369. doi: 10.1111/j.1432-1033.1977.tb11260.x. [DOI] [PubMed] [Google Scholar]
  8. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dudov K. P., Dabeva M. D., Hadjiolov A. A. Simple agar--urea-gel electrophoretic fractionation of high molecular weight ribonucleic acids. Anal Biochem. 1976 Nov;76(50):250–258. doi: 10.1016/0003-2697(76)90283-9. [DOI] [PubMed] [Google Scholar]
  10. Ferguson J., Davis R. W. A new electron microscopic technique for establishing the positions of genes: an analysis of the yeast ribosomal RNA coding region. J Mol Biol. 1978 Aug 15;123(3):417–430. doi: 10.1016/0022-2836(78)90088-8. [DOI] [PubMed] [Google Scholar]
  11. Fuke M., Busch H. Comparison of nucleotide sequences of large T1 ribonuclease fragments of 18S ribosomal RNA of rat and chicken. Nucleic Acids Res. 1979 Nov 10;7(5):1131–1135. doi: 10.1093/nar/7.5.1131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Glynn I. M., Chappell J. B. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity. Biochem J. 1964 Jan;90(1):147–149. doi: 10.1042/bj0900147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hagenbüchle O., Santer M., Steitz J. A., Mans R. J. Conservation of the primary structure at the 3' end of 18S rRNA from eucaryotic cells. Cell. 1978 Mar;13(3):551–563. doi: 10.1016/0092-8674(78)90328-8. [DOI] [PubMed] [Google Scholar]
  14. Kramer R. A., Philippsen P., Davis R. W. Divergent transcription in the yeast ribosomal RNA coding region as shown by hybridization to separated strands and sequence analysis of cloned DNA. J Mol Biol. 1978 Aug 15;123(3):405–416. doi: 10.1016/0022-2836(78)90087-6. [DOI] [PubMed] [Google Scholar]
  15. Laskey R. A., Mills A. D. Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film. FEBS Lett. 1977 Oct 15;82(2):314–316. doi: 10.1016/0014-5793(77)80609-1. [DOI] [PubMed] [Google Scholar]
  16. Maden B. E., Forbes J., de Jonge P., Klootwijk J. Presence of a hypermodified nucleotide in HeLa cell 18 S and Saccharomyces carlsbergensis 17 S ribosomal RNAs. FEBS Lett. 1975 Nov 1;59(1):60–63. doi: 10.1016/0014-5793(75)80341-3. [DOI] [PubMed] [Google Scholar]
  17. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Moss T., Boseley P. G., Birnstiel M. L. More ribosomal spacer sequences from Xenopus laevis. Nucleic Acids Res. 1980 Feb 11;8(3):467–485. doi: 10.1093/nar/8.3.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nath K., Bollon A. P. Generation of discrete yeast DNA fragments by endonuclease RI. Nature. 1975 Sep 11;257(5522):155–157. doi: 10.1038/257155a0. [DOI] [PubMed] [Google Scholar]
  20. Petes T. D., Broach J. R., Wensink P. C., Hereford L. M., Fink G. R., Botstein D. Isolation and analysis of recombinant DNA molecules containing yeast DNA. Gene. 1978 Sep;4(1):37–49. doi: 10.1016/0378-1119(78)90013-6. [DOI] [PubMed] [Google Scholar]
  21. Petes T. D., Hereford L. M., Skryabin K. G. Characterization of two types of yeast ribosomal DNA genes. J Bacteriol. 1978 Apr;134(1):295–305. doi: 10.1128/jb.134.1.295-305.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rubtsov P. M., Musakhanov M. M., Batchikova N. V., Skriabin K. S., Baev A. A. Opredelenie pervichnoi struktury fragmentov ribosomnogo operona pekarskikh drozhzhei, kodiruiushchikh 18 S rRNK. Dokl Akad Nauk SSSR. 1979;248(3):760–762. [PubMed] [Google Scholar]
  23. Samols D. R., Hagenbuchle O., Gage L. P. Homology of the 3' terminal sequences of the 18S rRNA of Bombyx mori and the 16S rRNA of Escherchia coli. Nucleic Acids Res. 1979 Nov 10;7(5):1109–1119. doi: 10.1093/nar/7.5.1109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sanger F., Coulson A. R. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. doi: 10.1016/0014-5793(78)80145-8. [DOI] [PubMed] [Google Scholar]
  25. Shine J., Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. doi: 10.1073/pnas.71.4.1342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Skriabin K. G., Kraev A. S., Rubtsov P. M., Baev A. A. Polnaia posledovatel'nost' nukleotidov speisernoi oblasti, raspolozhennoi mezhdu genami 18S i 5.8S RNK drozhzhei. Dokl Akad Nauk SSSR. 1979;247(3):761–765. [PubMed] [Google Scholar]
  27. Skryabin K. G., Maxam A. M., Petes T. D., Hereford L. Location of the 5.8S rRNA gene of Saccharomyces cerevisiae. J Bacteriol. 1978 Apr;134(1):306–309. doi: 10.1128/jb.134.1.306-309.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Szalay A. A., Grohmann K., Sinsheimer R. L. Separation of the complementary strands of DNA fragments on polyacrylamide gels. Nucleic Acids Res. 1977;4(5):1569–1578. doi: 10.1093/nar/4.5.1569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Tanaka T., Weisblum B. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J Bacteriol. 1975 Jan;121(1):354–362. doi: 10.1128/jb.121.1.354-362.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wellauer P. K., Dawid I. B., Brown D. D., Reeder R. H. The molecular basis for length heterogeneity in ribosomal DNA from Xenopus laevis. J Mol Biol. 1976 Aug 25;105(4):461–486. doi: 10.1016/0022-2836(76)90229-1. [DOI] [PubMed] [Google Scholar]

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