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Activity of His-tagged Ald at different pH levels. (A) Pyruvate reductive amination activity (circles) and alanine dehydrogenase activity (squares). (B) Glyoxylate reductive amination activity (circles), glycine dehydrogenase activity (squares), and glyoxylate reductive amination activity with glutamine in place of ammonium sulfate (triangles). Specific activity is shown in μmoles of NADH oxidized/min/μg of protein. The standard deviations are shown.
