Subcellular location of Ald. Cell extracts and culture filtrates from aerobically growing or NRP-2 cultures were probed with an anti-Ald antibody. (A) AG, aerobically growing cells; NRP, nonreplicating persistent stage 2 cells; F, growth medium filtrate; E, cell extracts. (B) W, cell wall fraction; S, soluble fraction; M, cell membrane.