FIGURE 3.
GCN5 increases both mRNA and protein levels of cyclin D1, cyclin E1, and E2F1. A and B, Western blotting analysis of the protein levels of cell cycle regulators in GCN5-knockdown A549 cells, or GCN5-overexpressing A549 cells and control cell lines. GAPDH was included to show that equivalent amounts of protein were loaded in each lane. A, proteins that are regulated by GCN5. B, proteins that are not regulated by GCN5. C, RT-qPCR analysis of cyclin D1, cyclin E1, and E2F1 mRNA in the GCN5-knockdown cells, GCN5-overexpressing A549 cells, and control cell lines. D, RT-qPCR analysis of apoptosis genes in GCN5-knockdown cells, GCN5-overexpressing cells, and control cell lines. *, p < 0.05; **, p < 0.01. Error bars, S.D.