Table 3.
Oligonucleotides used in this study.
| Name | Sequencea | Reference |
|---|---|---|
| TUP1_LHF _UPS | 5′- TTGGGCAGTGAGCTCTTTACTACTC -3′ | This study. |
| TUP1_LHF _DS | 5′- ATACATGGACATCCGCGGTGGGGATGG -3′ | This study. |
| TUP1_RHF_UPS | 5′- GGCGATTGTAACTCGAGAATTTGGAA -3′ | This study. |
| TUP1_RHF_UPS | 5′- GGGCCCTTCTCAAGTCTGCTAAACACG-3′ | This study. |
| TUP1_For | 5′- CTCGAGATGTCCATGTATCCCCAACG-3′ | This study. |
| TUP1_Rev | 5′- CAATTGCACTTATTTTTTGGTCCATTTCC-3′ | This study. |
| ACT1-S | 5′-ATGTGTAAAGCCGGTTTTGCCG-3′ | (Toyoda et al. 2004) |
| ACT1-A | 5′-CCATATCGTCCCAGTTGGAAAC-3′ | (Toyoda et al. 2004) |
| TUP1qFOR | 5′- GCCCATAGAAAAATCAAAGAGG-3′ | This Study. |
| TUP1qREV | 5′- GCCATTCTTCATTTGCCTGT-3′ | This Study. |
a
Underlined sequences indicate restriction enzyme recognition sites engineered into the oligonucleotides.