Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1991 Mar 15;88(6):2065–2069. doi: 10.1073/pnas.88.6.2065

FVB/N: an inbred mouse strain preferable for transgenic analyses.

M Taketo 1, A C Schroeder 1, L E Mobraaten 1, K B Gunning 1, G Hanten 1, R R Fox 1, T H Roderick 1, C L Stewart 1, F Lilly 1, C T Hansen 1, et al.
PMCID: PMC51169  PMID: 1848692

Abstract

FVB/N mice offer a system suitable for most transgenic experiments and subsequent genetic analyses. The inbred FVB/N strain is characterized by vigorous reproductive performance and consistently large litters. Moreover, fertilized FVB/N eggs contain large and prominent pronuclei, which facilitate microinjection of DNA. The phenotype of large pronuclei in the zygote is a dominant trait associated with the FVB/N oocyte but not the FVB/N sperm. In experiments to generate transgenic mice, the same DNA constructs were injected into three different types of zygotes: FVB/N, C57BL/6J, and (C57BL/6J x SJL/J)F1. FVB/N zygotes survived well after injection, and transgenic animals were obtained with efficiencies similar to the F1 zygotes and much better than the C57BL/6J zygotes. Genetic markers of the FVB/N strain have been analyzed for 44 loci that cover 15 chromosomes and were compared with those of commonly used inbred strains. In addition to the albino FVB/N strain, pigmented congenic strains of FVB/N are being constructed. These features make the FVB/N strain advantageous to use for research with transgenic mice.

Full text

PDF
2065

Images in this article

2067Image
on p.2067
2067Image
on p.2067

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brinster R. L., Chen H. Y., Trumbauer M. E., Yagle M. K., Palmiter R. D. Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. Proc Natl Acad Sci U S A. 1985 Jul;82(13):4438–4442. doi: 10.1073/pnas.82.13.4438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gossler A., Doetschman T., Korn R., Serfling E., Kemler R. Transgenesis by means of blastocyst-derived embryonic stem cell lines. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9065–9069. doi: 10.1073/pnas.83.23.9065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Grosschedl R., Weaver D., Baltimore D., Costantini F. Introduction of a mu immunoglobulin gene into the mouse germ line: specific expression in lymphoid cells and synthesis of functional antibody. Cell. 1984 Oct;38(3):647–658. doi: 10.1016/0092-8674(84)90259-9. [DOI] [PubMed] [Google Scholar]
  4. Jolicoeur P. The Fv-1 gene of the mouse and its control of murine leukemia virus replication. Curr Top Microbiol Immunol. 1979;86:67–122. doi: 10.1007/978-3-642-67341-2_3. [DOI] [PubMed] [Google Scholar]
  5. Leonard J. M., Abramczuk J. W., Pezen D. S., Rutledge R., Belcher J. H., Hakim F., Shearer G., Lamperth L., Travis W., Fredrickson T. Development of disease and virus recovery in transgenic mice containing HIV proviral DNA. Science. 1988 Dec 23;242(4886):1665–1670. doi: 10.1126/science.3201255. [DOI] [PubMed] [Google Scholar]
  6. Mahon K. A., Chepelinsky A. B., Khillan J. S., Overbeek P. A., Piatigorsky J., Westphal H. Oncogenesis of the lens in transgenic mice. Science. 1987 Mar 27;235(4796):1622–1628. doi: 10.1126/science.3029873. [DOI] [PubMed] [Google Scholar]
  7. Mahon K. A., Overbeek P. A., Westphal H. Prenatal lethality in a transgenic mouse line is the result of a chromosomal translocation. Proc Natl Acad Sci U S A. 1988 Feb;85(4):1165–1168. doi: 10.1073/pnas.85.4.1165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Overbeek P. A., Chepelinsky A. B., Khillan J. S., Piatigorsky J., Westphal H. Lens-specific expression and developmental regulation of the bacterial chloramphenicol acetyltransferase gene driven by the murine alpha A-crystallin promoter in transgenic mice. Proc Natl Acad Sci U S A. 1985 Dec;82(23):7815–7819. doi: 10.1073/pnas.82.23.7815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
  10. Schroeder A. C., Eppig J. J. The developmental capacity of mouse oocytes that matured spontaneously in vitro is normal. Dev Biol. 1984 Apr;102(2):493–497. doi: 10.1016/0012-1606(84)90215-x. [DOI] [PubMed] [Google Scholar]
  11. Silva C. M., Tully D. B., Petch L. A., Jewell C. M., Cidlowski J. A. Application of a protein-blotting procedure to the study of human glucocorticoid receptor interactions with DNA. Proc Natl Acad Sci U S A. 1987 Apr;84(7):1744–1748. doi: 10.1073/pnas.84.7.1744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Thomas S. M., Moreno R. F., Tilzer L. L. DNA extraction with organic solvents in gel barrier tubes. Nucleic Acids Res. 1989 Jul 11;17(13):5411–5411. doi: 10.1093/nar/17.13.5411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wagner E. F., Keller G., Gilboa E., Rüther U., Stewart C. Gene transfer into murine stem cells and mice using retroviral vectors. Cold Spring Harb Symp Quant Biol. 1985;50:691–700. doi: 10.1101/sqb.1985.050.01.085. [DOI] [PubMed] [Google Scholar]
  14. Woychik R. P., Stewart T. A., Davis L. G., D'Eustachio P., Leder P. An inherited limb deformity created by insertional mutagenesis in a transgenic mouse. Nature. 1985 Nov 7;318(6041):36–40. doi: 10.1038/318036a0. [DOI] [PubMed] [Google Scholar]
  15. Yokoyama T., Silversides D. W., Waymire K. G., Kwon B. S., Takeuchi T., Overbeek P. A. Conserved cysteine to serine mutation in tyrosinase is responsible for the classical albino mutation in laboratory mice. Nucleic Acids Res. 1990 Dec 25;18(24):7293–7298. doi: 10.1093/nar/18.24.7293. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES

  NODES
twitter 2