Figure 4.
Inhibitory activity in primary human monocytes. (a) Human PBMCs isolated from peripheral blood were stimulated with LPS (100 ng/ml) for 4 h in the presence of increasing concentrations of SP600125. Cell lysates were prepared and analyzed by using multiplex mRNA detection (Systems Integration Drug Discovery Company). Results are expressed as the mean of triplicate determinations. (b) Primary human monocytes were isolated by positive selection (CD14) and stimulated with LPS (100 ng/ml) in the presence of increasing concentrations of SP600125. After 15 h, culture supernatants were analyzed for expression of IL-1β, -8, and TNF-α with an ELISA. Values are mean and SD for quadruplicate samples.