Figure 2.
AT Prevents Infection with SARS-CoV-2
Human Caco-2 cells were pretreated with 10 or 25 μM AT for 4 h or left untreated and were infected with SARS-CoV-2 with a multiplicity of infection (MOI) of 0.001 in the presence or absence of AT. The titer of progeny in the culture supernatants was determined 24 h post infection (p.i.) by plaque assay. Viral transcripts were also detected by quantitative real-time RT-PCR with two separate primer sets in the lysates of Caco-2 cells treated as above but infected with a MOI of 0.1 for 24 h. Shown are the means ± SD of 3 experiments; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 compared to mock or infected without AT; Dunnets (for plaque-forming units [PFUs]) or Sidak’s (for PCR) multiple comparisons test.