ࡱ > w y v #` bjbjmm 8z @ @ @ @ @ @ @ T . . . 8 . . T D9 x0 x0 x0 x0 x0 S1 S1 S1 8 8 8 8 8 8 8 $ (: h < 8 @ S1 S1 S1 S1 S1 8 @ @ x0 x0 8 2 2 2 S1 j @ x0 @ x0 8 2 S1 8 2 2 @ @ 2 x0 l0 d0} . 1 ^ 2 3 9 0 D9 2 = 2 | = 2 = @ 2 ( S1 S1 2 S1 S1 S1 S1 S1 8 8 2 X S1 S1 S1 D9 S1 S1 S1 S1 T d D $ d T T T T @ @ @ @ @ @ Table S2: Percentage of sequences remaining after quality filtering applying all criteria or a single criterion
Percentage of pyrosequences remaining after quality filteringSamplePrimerAll criteriaExact matches to the forward primer
No ambiguous basses (N)
Read-lengths not longer or shorter than 1 SD from the average sequence length
F127F-DegL50.7291.8485.1964.3627F-DegS49.8791.2083.4565.4527F-Nondeg48.5395.0885.5360.4935F-Nondeg47.3896.2885.9157.98F227F-DegL52.1092.2984.4566.4027F-DegS50.0689.0183.4565.8627F-Nondeg49.3995.8684.4860.9035F-Nondeg51.3295.7886.1461.88F327F-DegL54.2792.7488.1265.6827F-DegS52.0592.5286.8263.4527F-Nondeg48.5396.2688.1756.3335F-Nondeg47.8093.5287.6156.98S127F-DegL48.2692.3683.4064.3627F-DegS49.7792.5984.5665.2727F-Nondeg48.5896.2183.1462.8235F-Nondeg51.1795.6387.2462.50S227F-DegL50.3892.6284.6864.6927F-DegS49.0492.1384.5563.8727F-Nondeg49.1996.2684.8661.6035F-Nondeg43.8088.2085.7960.23S327F-DegL52.7991.2985.0866.1727F-DegS53.1692.3986.5364.2227F-Nondeg47.6094.3586.4557.3535F-Nondeg48.8595.8085.3859.21
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