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. 1996 Oct 15;24(20):4071–4077. doi: 10.1093/nar/24.20.4071

Genomic structure and expression of the murine Hmgi-c gene.

X Zhou 1, K F Benson 1, K Przybysz 1, J Liu 1, Y Hou 1, L Cherath 1, K Chada 1
PMCID: PMC146186  PMID: 8918814

Abstract

The murine Hmgi-c gene, a member of the Hmgi gene family, contains five exons encompassing >110 kb of genomic DNA at the pygmy locus on mouse chromosome 10. Northern analysis identified a 4.1 kb transcript which contains a 324 bp open reading frame encoding a 12 kDa HMGI-C protein. Further analysis defined both the 5' and 3' untranslated regions of the Hmgi-c mRNA species as 658 and 2967 bp respectively. The HMGI-C protein has three consecutive AT hook DNA binding domains and an acidic domain, each of which are encoded by individual exons; such an organization is conserved among the HMGI gene family members from insects to mammals. Similar to the HMGI/Y proteins, the HMGI-C protein does not function as a typical transcriptional activator. Developmental studies revealed that the Hmgi-c gene is expressed predominantly during mouse embryogenesis. Since the human homolog is disrupted in a number of tumors, HMGI-C could play an important role in cell proliferation and differentiation during mammalian development.

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