Figure 7

Expression of modified lipoprotein receptors and uptake of Ac-LDL are increased in peritoneal macrophages. (A) mRNA expression of CD36 and SR-A by real-time PCR in peritoneal macrophages from Ldlr–/– and hAR-Ldlr–/– mice. Values were normalized to β-actin and expressed as mean ± SEM. Data are from experiments performed in duplicate on an average of 8 mice. (B) Western blot analysis of CD36, SR-A, and ATP-binding cassette A1 (ABCA1) protein in peritoneal macrophages from hAR-Ldlr–/– and Ldlr–/– mice. Data are from pooled macrophages collected from 3 mice per group. (C) Uptake of 125I-Ac-LDL (100 μg/ml) in peritoneal macrophages from Ldlr–/– and hAR-Ldlr–/– mice. Values are expressed as mean ± SEM. Data are from experiments performed in duplicate on 6 mice. *P < 0.05.