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Br J Cancer. 1980 Dec; 42(6): 908–914.
doi: 10.1038/bjc.1980.339
PMCID: PMC2010586
PMID: 6936052

Characterization of gangliosides in human leucocytes.

A. Tsuboyama, F. Takaku, S. Sakamoto, Y. Kano, T. Ariga, and T. Miyatake
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Abstract

Lipids extracted from leucocyte pellets with chloroform-methanol were applied to a DEAE-Sephadex column an the gangliosides were eluted with 0.2M sodium acetate in methanol. The eluate was desalted by Sephadex G-50 column chromatography. The purified ganglioside was spotted on the high-performance than-layer plate. The plate was developed and sprayed with resorcinol reagents and heated. Seventeen bands of gangliosides were demonstrated in normal human leucocytes. The composition of these gangliosides was different in the various kinds of leucocytes. Amounts of GM3 ganglioside were apparently greater in normal lymphocytes and leukaemic cells than in granulocytes. Among the acute-leukaemic cells, some kinds of complex gangliosides were much more abundant in myelogenous cells than in lymphocytic cells. These changes in ganglioside composition are suggested as new biochemical markers for leukaemic cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  • Ando S, Chang NC, Yu RK. High-performance thin-layer chromatography and densitometric determination of brain ganglioside compositions of several species. Anal Biochem. 1978 Sep;89(2):437–450. [PubMed] [Google Scholar]
  • Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  • Dacremont G, Hildebrand J. Characterization of two gangliosides from human leukemic polymorphonuclear leukocytes. Biochim Biophys Acta. 1976 Feb 23;424(2):315–322. [PubMed] [Google Scholar]
  • Greaves MF. Clinical applications of cell surface markers. Prog Hematol. 1975;9:255–303. [PubMed] [Google Scholar]
  • Hakomori S. Structures and organization of cell surface glycolipids dependency on cell growth and malignant transformation. Biochim Biophys Acta. 1975 Mar 20;417(1):55–89. [PubMed] [Google Scholar]
  • Hildebrand J, Stryckmans PA, Vanhouche J. Gangliosides in leukemic and non-leukemic human leukocytes. Biochim Biophys Acta. 1972 Feb 21;260(2):272–278. [PubMed] [Google Scholar]
  • Hollenberg MD, Fishman PH, Bennett V, Cuatrecasas P. Cholera toxin and cell growth: role of membrane gangliosides. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4224–4228. [PMC free article] [PubMed] [Google Scholar]
  • Rosenfelder G, Young WW, Jr, Hakomori SI. Association of the glycolipid pattern with antigenic alterations in mouse fibroblasts transformed by murine sarcoma virus. Cancer Res. 1977 May;37(5):1333–1339. [PubMed] [Google Scholar]
  • Sarin PS, Anderson PN, Gallo RC. Terminal deoxynucleotidyl transferase activities in human blood leukocytes and lymphoblast cell lines: high levels in lymphoblast cell lines and in blast cells of some patients with chronic myelogenous leukemia in acute phase. Blood. 1976 Jan;47(1):11–20. [PubMed] [Google Scholar]
  • Stein KE, Marcus DM. Glycosphingolipids of purified human lymphocytes. Biochemistry. 1977 Nov 29;16(24):5285–5291. [PubMed] [Google Scholar]
  • SVENNERHOLM L. Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. Biochim Biophys Acta. 1957 Jun;24(3):604–611. [PubMed] [Google Scholar]
  • SVENNERHOLM L. CHROMATOGRAPHIC SEPARATION OF HUMAN BRAIN GANGLIOSIDES. J Neurochem. 1963 Sep;10:613–623. [PubMed] [Google Scholar]
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