doi: 10.1021/bi00679a001.
Ribosomal subunit interaction as studied by light scattering. Evidence of different classes of ribosome preparations
- PMID: 1092339
- DOI: 10.1021/bi00679a001
Item in Clipboard
Ribosomal subunit interaction as studied by light scattering. Evidence of different classes of ribosome preparations
Biochemistry.
.
Abstract
The Mg2+-dependent equilibrium of ribosomal subunits was studied by light scattering in the absence of any other factor of protein synthesis. The difference of intensity in the light scattered by dissociated 30S-50S couples and by 70S particles was used as a method to measure the percentage of association as a function of Mg2+ under conditions of nonperturbation of the equilibrium. We found that pressure-resistant (type A) and non-pressure-resistant (type B) ribosomes may be characterized by the different behavior of their association equilibrium curves. The thermodynamic parameters of this equilibrium and their comparison for the two classes of ribosomes were calculated from experiments at different temperatures.
Similar articles
-
Kinetics of ribosome dissociation and subunit association studied in a light-scattering stopped-flow apparatus.Biochemistry. 1976 Dec 28;15(26):5743-53. doi: 10.1021/bi00671a010. Biochemistry. 1976. PMID: 795460
-
Relaxation kinetics of E. coli ribosomes: evidence for the reaction of 30S . IF3 complex with 50S ribosomal subunits.Biophys Chem. 1979 May;9(4):405-12. Biophys Chem. 1979. PMID: 380673
-
Thermodynamic studies of the reversible association of Escherichia coli ribosomal subunits.Biochemistry. 1977 Jun 14;16(12):2800-5. doi: 10.1021/bi00631a034. Biochemistry. 1977. PMID: 329865
-
Magnesium dependence of the association kinetics of Escherichia coli ribosomal subunits.Biochemistry. 1976 Sep 7;15(18):3903-12. doi: 10.1021/bi00663a001. Biochemistry. 1976. PMID: 786363
-
Magnesium ion dependent equilibria, kinetics, and thermodynamic parameters of Artemia ribosome dissociation and subunit association.Biochemistry. 1986 Jun 17;25(12):3690-5. doi: 10.1021/bi00360a032. Biochemistry. 1986. PMID: 3718953
Cited by
-
Formylation of initiator tRNA methionine in procaryotic protein synthesis: in vivo polarity in lactose operon expression.J Bacteriol. 1978 Aug;135(2):453-9. doi: 10.1128/jb.135.2.453-459.1978. J Bacteriol. 1978. PMID: 98518 Free PMC article.
-
The Discovery of Ribosomal Protein bL31 from Escherichia coli: A Long Story Revisited.Int J Mol Sci. 2023 Feb 8;24(4):3445. doi: 10.3390/ijms24043445. Int J Mol Sci. 2023. PMID: 36834855 Free PMC article. Review.
-
Covalent crosslinking of tRNA1Val to 16S RNA at the ribosomal P site: identification of crosslinked residues.Proc Natl Acad Sci U S A. 1982 Sep;79(18):5450-4. doi: 10.1073/pnas.79.18.5450. Proc Natl Acad Sci U S A. 1982. PMID: 6813860 Free PMC article.
-
Effects of macromolecular crowding on the association of E. coli ribosomal particles.Nucleic Acids Res. 1988 Jul 25;16(14A):6309-26. doi: 10.1093/nar/16.14.6309. Nucleic Acids Res. 1988. PMID: 3041372 Free PMC article.
-
A new ribosomal mutation which affects the two ribosomal subunits in Escherichia coli.Mol Gen Genet. 1977 Jun 8;153(2):199-204. doi: 10.1007/BF00264736. Mol Gen Genet. 1977. PMID: 329117