Distinct molecular mechanisms and divergent endocytotic pathways of AMPA receptor internalization
- PMID: 11100149
- DOI: 10.1038/81814
Distinct molecular mechanisms and divergent endocytotic pathways of AMPA receptor internalization
Abstract
Internalization of postsynaptic AMPA receptors depresses excitatory transmission, but the underlying dynamics and mechanisms of this process are unclear. Using immunofluorescence and surface biotinylation, we characterized and quantified basal and regulated AMPA receptor endocytosis in cultured hippocampal neurons, in response to synaptic activity, AMPA and insulin. AMPA-induced AMPA receptor internalization is mediated in part by secondary activation of voltage-dependent calcium channels, and in part by ligand binding independent of receptor activation. Although both require dynamin, insulin- and AMPA-induced AMPA receptor internalization are differentially dependent on protein phosphatases and sequence determinants within the cytoplasmic tails of GluR1 and GluR2 subunits. AMPA receptors internalized in response to AMPA stimulation enter a recycling endosome system, whereas those internalized in response to insulin diverge into a distinct compartment. Thus, the molecular mechanisms and intracellular sorting of AMPA receptors are diverse, and depend on the internalizing stimulus.
Similar articles
-
Regulation of AMPA receptor endocytosis by a signaling mechanism shared with LTD.Nat Neurosci. 2000 Dec;3(12):1291-300. doi: 10.1038/81823. Nat Neurosci. 2000. PMID: 11100150
-
An investigation into signal transduction mechanisms involved in insulin-induced long-term depression in the CA1 region of the hippocampus.J Neurochem. 2004 Apr;89(1):217-31. doi: 10.1111/j.1471-4159.2003.02307.x. J Neurochem. 2004. PMID: 15030406
-
Subunit rules governing the sorting of internalized AMPA receptors in hippocampal neurons.Neuron. 2004 Jul 22;43(2):221-36. doi: 10.1016/j.neuron.2004.06.015. Neuron. 2004. PMID: 15260958
-
Regulation of AMPA receptors and synaptic plasticity.Neuroscience. 2009 Jan 12;158(1):105-25. doi: 10.1016/j.neuroscience.2008.02.037. Epub 2008 Feb 29. Neuroscience. 2009. PMID: 18424006 Review.
-
New insights in endosomal dynamics and AMPA receptor trafficking.Semin Cell Dev Biol. 2011 Jul;22(5):499-505. doi: 10.1016/j.semcdb.2011.06.008. Epub 2011 Aug 6. Semin Cell Dev Biol. 2011. PMID: 21843653 Review.
Cited by
-
Enhancement of dynamin polymerization and GTPase activity by Arc/Arg3.1.Biochim Biophys Acta. 2015 Jun;1850(6):1310-8. doi: 10.1016/j.bbagen.2015.03.002. Epub 2015 Mar 14. Biochim Biophys Acta. 2015. PMID: 25783003 Free PMC article.
-
Endocytosis and degradative sorting of NMDA receptors by conserved membrane-proximal signals.J Neurosci. 2004 Aug 11;24(32):7096-109. doi: 10.1523/JNEUROSCI.0780-04.2004. J Neurosci. 2004. PMID: 15306643 Free PMC article.
-
UPF2 leads to degradation of dendritically _targeted mRNAs to regulate synaptic plasticity and cognitive function.Mol Psychiatry. 2020 Dec;25(12):3360-3379. doi: 10.1038/s41380-019-0547-5. Epub 2019 Oct 21. Mol Psychiatry. 2020. PMID: 31636381 Free PMC article.
-
Glutamatergic ionotropic blockade within accumbens disrupts working memory and might alter the endocytic machinery in rat accumbens and prefrontal cortex.J Neural Transm (Vienna). 2007;114(12):1519-28. doi: 10.1007/s00702-007-0776-7. Epub 2007 Jul 10. J Neural Transm (Vienna). 2007. PMID: 17616844
-
Insulin-like growth factor 1 regulates excitatory synaptic transmission in pyramidal neurons from adult prefrontal cortex.Neuropharmacology. 2022 Oct 1;217:109204. doi: 10.1016/j.neuropharm.2022.109204. Epub 2022 Aug 2. Neuropharmacology. 2022. PMID: 35931212 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
