Promoter-specific binding of Rap1 revealed by genome-wide maps of protein-DNA association
- PMID: 11455386
- DOI: 10.1038/ng569
Promoter-specific binding of Rap1 revealed by genome-wide maps of protein-DNA association
Erratum in
- Nat Genet 2001 Sep;29(1):100
Abstract
We determined the distribution of repressor-activator protein 1 (Rap1) and the accessory silencing proteins Sir2, Sir3 and Sir4 in vivo on the entire yeast genome, at a resolution of 2 kb. Rap1 is central to the cellular economy during rapid growth, _targeting 294 loci, about 5% of yeast genes, and participating in the activation of 37% of all RNA polymerase II initiation events in exponentially growing cells. Although the DNA sequence recognized by Rap1 is found in both coding and intergenic sequences, the binding of Rap1 to the genome was highly specific to intergenic regions with the potential to act as promoters. This global phenomenon, which may be a general characteristic of sequence-specific transcriptional factors, indicates the existence of a genome-wide molecular mechanism for marking promoter regions.
Comment in
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To bind or not to bind.Nat Genet. 2001 Aug;28(4):303-4. doi: 10.1038/91045. Nat Genet. 2001. PMID: 11479583
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