doi: 10.1111/j.1432-1033.1975.tb02447.x.
Studies on the role and mode of operation of the very-lysine-rich histones in eukaryote chromatin. The conformation of phi1 histones from marine invertebrate sperm
- PMID: 1204610
- DOI: 10.1111/j.1432-1033.1975.tb02447.x
Item in Clipboard
Studies on the role and mode of operation of the very-lysine-rich histones in eukaryote chromatin. The conformation of phi1 histones from marine invertebrate sperm
Eur J Biochem.
.
Free article
Abstract
Proton magnetic resonance, circular dichroism and infrared spectroscopy are used to investigate the secondary and tertiary structure of three very lysine-rich histones from marine invertebrate sperm. At high ionic strength both Arbacia lixula and Holothuria tubulosa histone phi 1 are observed to contain 25-30% alpha-helix, no beta-structure and to form specific folded structures. Both phi 1 proton magnetic resonance spectra have perturbed methyl resonances at chemical shifts close to those observed for calf thymus H1, suggesting analogies in tertiary structure. Mytilus edulis histone phi 1 however, shows no spectroscopic evidence of secondary and tertiary structure on salt addition.
Similar articles
-
The structure of sea-urchin-sperm histone phi 1 (H1) in chromatin and in free solution. Trypsin digestion and spectroscopic studies.Eur J Biochem. 1980 Feb;104(1):263-70. doi: 10.1111/j.1432-1033.1980.tb04424.x. Eur J Biochem. 1980. PMID: 6768551
-
Studies on the role and mode of operation of the very-lysine-rich histones in eukaryote chromatin. Nuclear-magnetic-resonance studies on nucleoprotein and histone phi 1-DNA complexes from marine invertebrate sperm.Eur J Biochem. 1976 Jun 1;65(2):357-63. doi: 10.1111/j.1432-1033.1976.tb10349.x. Eur J Biochem. 1976. PMID: 985747
-
Antigenic variability among very-lysine-rich histones from calf thymus and from sperm of different echinoderms.Comp Biochem Physiol B. 1983;74(3):611-7. doi: 10.1016/0305-0491(83)90237-7. Comp Biochem Physiol B. 1983. PMID: 6404593
-
Heterogeneity of the histone-containing chromatin in sea cucumber spermatozoa. Distribution of the basic protein phi 0 and absence of non-histone proteins.Exp Cell Res. 1983 Oct 15;148(2):331-44. doi: 10.1016/0014-4827(83)90157-x. Exp Cell Res. 1983. PMID: 6628561
-
Recent developments in the field of marine natural products with emphasis on biologically active compounds.Fortschr Chem Org Naturst. 1986;49:151-363. doi: 10.1007/978-3-7091-8846-0_3. Fortschr Chem Org Naturst. 1986. PMID: 2877925 Review. No abstract available.
Cited by
-
Interaction between domains in chromosomal protein HMG-1.EMBO J. 1984 Jun;3(6):1255-61. doi: 10.1002/j.1460-2075.1984.tb01960.x. EMBO J. 1984. PMID: 6086312 Free PMC article.
-
Preparation and characterization of histone H1 from the sperm of the sea-urchin Sphaerechinus granularis.Biochem J. 1981 Apr 1;195(1):171-6. doi: 10.1042/bj1950171. Biochem J. 1981. PMID: 7306043 Free PMC article.
