Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Feb;162(2):609-17.
doi: 10.1016/S0002-9440(10)63854-0.

Tumor suppressor role of KiSS-1 in bladder cancer: loss of KiSS-1 expression is associated with bladder cancer progression and clinical outcome

Marta Sanchez-Carbayo  1 , Paola Capodieci, Carlos Cordon-Cardo
Affiliations

Tumor suppressor role of KiSS-1 in bladder cancer: loss of KiSS-1 expression is associated with bladder cancer progression and clinical outcome

Marta Sanchez-Carbayo et al. Am J Pathol. 2003 Feb.

Abstract

The expression profiles of nine bladder cancer cell lines were compared against a pool containing equal total RNA quantities of each of them. Lower expression of KiSS-1 was revealed in cells derived from the most advanced bladder tumors. When comparing 15 primary bladder tumors versus a pool of four bladder cancer cell lines, lower transcript levels of KiSS-1 were observed in the invasive bladder carcinomas as compared to superficial tumors. KiSS-1 expression ratios provided prognostic information. The expression pattern of KiSS-1 transcripts was analyzed using in situ hybridization in nine bladder cancer cells, paired normal urothelium and bladder tumor samples (n = 25), and tissue microarrays of bladder tumors (n = 173). We observed complete loss of KiSS-1 in all invasive tumors under study as compared to their respective normal urothelium. The expression of KiSS-1 was found to be significantly associated with histopathological stage. Patients with lower KiSS-1 expression showed a direct correlation with overall survival in a subset of bladder tumors whose follow-up was available (n = 69). We did not observe any significant differential KiSS-1 expression along cell cycle by sorting analysis. A potential tumor suppressor role in bladder cancer was revealed for KiSS-1. Moreover, it showed predictive value by identifying patients with poor outcome.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Kaplan-Meier curves obtained from the survival analysis of R/G ratios of KiSS-1 in the patients with bladder tumors to whom cDNA microarrays were performed. Low expression of KiSS-1 is represented by bladder tumors displaying a R/G ratio <1.5 versus those patients with high expression of KiSS-1 and R/G ratio >1.5. KiSS-1 was found to be significantly associated with overall survival in this subset of 15 bladder lesions whose expression profiles were analyzed (P = 0.008) (median follow-up time, 2 months). No association with overall survival was found for the R/G ratios of 6q16.2-21.
Figure 2.
Figure 2.
Representative expression patterns of KiSS-1 in normal urothelium (A), and transitional superficial (B) and invasive (C) bladder tumors. High expression levels of KiSS-1 were observed in normal urothelium and superficial bladder tumors as compared to the invasive bladder tumor. There was a significant difference regarding the expression of KiSS-1 regarding tumor stage and grade. Original magnifications, ×200.
Figure 3.
Figure 3.
Kaplan-Meier curve obtained from the survival analysis of the expression of KiSS-1 in a subset of 69 bladder lesions contained in a tissue microarray. KiSS-1 expression was analyzed by in situ hybridization in this separate subset of patients whose median follow-up time of 36 months. KiSS-1 was found to be significantly associated with overall survival (P = 0.03).
Figure 4.
Figure 4.
A: Distribution of cell-cycle phases obtained from the cell-sorting analysis of RT4 and SCaBER. Cell-cycle-specific cytospins including cell-cycle subpopulations were obtained to evaluate the expression of KiSS-1 along cell cycle by in situ hybridization. B: Representative patterns of KiSS-1 in bladder cancer cell lines under study by in situ hybridization derived from a transitional superficial tumor (RT4) and a squamous bladder tumor (SCaBER). No differential expression of KiSS-1 by in situ hybridization was observed in these sorted cells spotted on cytospins containing enriched populations of each cell cycle phase. Original magnifications, ×200.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. : Cancer Facts and Figures 2000.: American Cancer Society 2000,
    1. Fidler IJ: Critical factors in the biology of human cancer metastasis. Cancer Res 1990, 50:6130-6138 - PubMed
    1. Welch DR, Chen P, Miele ME, McGary CT, Bower JM, Weissman BE, Standbridge EJ: Microcell-mediated transfer of chromosome 6 into metastatic human C8161 melanoma cells suppresses metastasis but does not inhibit tumorigenicity. Oncogene 1994, 9:255-262 - PubMed
    1. Miele ME, Robertson G, Lee JH, Coleman A, McGarry CT, Fisher PB, Lugo TG, Welch DR: Metastasis suppressed, but tumorigenicity and local invasiveness unaffected in the human melanoma cell line MelJuSo after introduction of human chromosomes 1 or 6. Mol Carcinog 1996, 15:284-299 - PubMed
    1. Lee JH, Miele ME, Hicks DJ, Phillips KK, Trent JM, Weissman BE, Welch DR: KiSS-1, a novel human malignant melanoma metastasis-suppressor gene. J Natl Cancer Inst 1996, 88:1731-1737 - PubMed

MeSH terms

  NODES
Association 1
twitter 2