Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2003 Jul;47(7):2217-22.
doi: 10.1128/AAC.47.7.2217-2222.2003.

In vitro and in vivo activities of fluoroquinolones against Aeromonas hydrophila

Wen-Chien Ko  1 , Shyh-Ren Chiang, Hsin-Chun Lee, Hung-Jen Tang, Yin-Yi Wang, Yin-Ching Chuang
Affiliations
Comparative Study

In vitro and in vivo activities of fluoroquinolones against Aeromonas hydrophila

Wen-Chien Ko et al. Antimicrob Agents Chemother. 2003 Jul.

Abstract

Aeromonas hydrophila, an uncommon human pathogen, can cause invasive infections in immunocompromised individuals. As the fluoroquinolones have been shown to be active in vitro against mesophilic aeromonads and clinical experience with the use of fluoroquinolones to treat aeromonads infections is limited, the antimicrobial activities of five selected drugs (ciprofloxacin, gatifloxacin, levofloxacin, lomefloxacin, and moxifloxacin) against A. hydrophila were studied in vitro and in mice. The MICs of the fluoroquinolones (except lomefloxacin), cefotaxime, and minocycline for 90% of 64 clinical isolates of A. hydrophila tested by the agar dilution method were <or=1 microg/ml. With a clinical cefotaxime-resistant strain, Ah 2743, in an in vitro time-kill study, at an inoculum of 7 x 10(5) CFU/ml incubated with fluoroquinolones, cefotaxime, or minocycline at concentrations equal to twice the MICs, the inhibitory effect lasted for less than 6 h and regrowth occurred thereafter. In an animal model with female BALB/c mice intraperitoneally infected with an inoculum of 1.1 x 10(7) CFU of Ah 2743, more mice in the ciprofloxacin-treated group survived (72.2%) than in the cefotaxime-, minocycline-, or cefotaxime-minocycline-treated group (P < 0.00001, log rank test). However, there were similar fatality rates, ranging from 71.4 to 87.5%, among mice treated with any of five fluoroquinolones. With a larger inoculum, 4.9 x 10(7) CFU, mice in the ciprofloxacin-treated group survived longer than those in the minocycline-, cefotaxime-, or cefotaxime-minocycline-treated group (30 h versus 18, 12, and 12 h, respectively [P < 0.002, log rank test]). However, in mice infected with cefotaxime-susceptible Ah 2556, ciprofloxacin was as effective as cefotaxime-minocycline. Thus, our results suggest that ciprofloxacin is at least as effective as cefotaxime-minocycline against murine A. hydrophila infections, which warrants clinical studies to delineate its role in human infections.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Time-kill curves of Ah 2743 at an initial inoculum of 7 × 105 CFU/ml after incubation with various drugs at a concentration equal to twice the MIC. L, liter.
FIG. 2.
FIG. 2.
Time-kill curves of Ah 2743 at an initial inoculum of 5.9 × 105 CFU/ml after incubation with fluoroquinolones at a concentration equal to twice the MIC. L, liter.
FIG. 3.
FIG. 3.
Kaplan-Meier survival curves of five groups of mice infected with two inocula of Ah 2743, a cefotaxime-resistant bacteremic isolate, and treated with different antibiotics. (a) Inoculum of 1.1 × 107 CFU. (b) Inoculum of 4.9 × 107 CFU. The mice in all of the experimental groups, regardless of antimicrobial therapy, died within 36 h.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Altweg M. 1999. Aeromonas and Plesiomonas, p. 507-516. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of clinical microbiology, 7th ed. American Society for Microbiology, Washington, D.C.
    1. Burgos, A., G. Quindos, R. Martinez, P. Rojo, and R. Cisterna. 1990. In vitro susceptibility of Aeromonas caviae, Aeromonas hydrophila and Aeromonas sobria to fifteen antibacterial agents. Eur. J. Clin. Microbiol. Infect. Dis 9:413-417. - PubMed
    1. Chuang, Y. C., C. Y. Yuan, C. Y. Liu, C. K. Lan, and A. H. M. Huang. 1992. Vibrio vulnificus infection in Taiwan: report of 28 cases and review of clinical manifestations and treatment. Clin. Infect. Dis. 15:271-276. - PubMed
    1. Clark, R. B. 1992. Antibiotic susceptibilities of the Vibrionaceae to meropenem and other antimicrobial agents. Diagn. Microbiol. Infect. Dis. 15:453-455. - PubMed
    1. Dryden, M., and R. Munro. 1989. Aeromonas septicemia: relationship of species and clinical features. Pathology 21:111-114. - PubMed

Publication types

MeSH terms

  NODES
INTERN 2
twitter 2