Regulation of human embryonic stem cell differentiation by BMP-2 and its antagonist noggin
- PMID: 14996946
- DOI: 10.1242/jcs.00970
Regulation of human embryonic stem cell differentiation by BMP-2 and its antagonist noggin
Abstract
Human embryonic stem cells differentiate spontaneously in vitro into a range of cell types, and they frequently give rise to cells with the properties of extra-embryonic endoderm. We show here that endogenous signaling by bone morphogenetic protein-2 controls the differentiation of embryonic stem cells into this lineage. Treatment of embryonic stem cell cultures with the bone morphogenetic protein antagonist noggin blocks this form of differentiation and induces the appearance of a novel cell type that can give rise to neural precursors. These findings indicate that bone morphogenetic protein-2 controls a key early commitment step in human embryonic stem cell differentiation, and show that the conservation of developmental mechanisms at the cellular level can be exploited in this system--in this case, to provide a facile route for the generation of neural precursors from pluripotent cells.
Similar articles
-
Essential requirement of BMPs-2/4 for both osteoblast and osteoclast formation in murine bone marrow cultures from adult mice: antagonism by noggin.J Bone Miner Res. 2000 Apr;15(4):663-73. doi: 10.1359/jbmr.2000.15.4.663. J Bone Miner Res. 2000. PMID: 10780858
-
Bone morphogenetic proteins and growth and differentiation factors in the human cornea.Invest Ophthalmol Vis Sci. 1999 Feb;40(2):296-311. Invest Ophthalmol Vis Sci. 1999. PMID: 9950587
-
Bone morphogenetic protein regulation of forkhead/winged helix transcription factor Foxc2 (Mfh1) in a murine mesodermal cell line C1 and in skeletal precursor cells.J Bone Miner Res. 2001 Oct;16(10):1765-71. doi: 10.1359/jbmr.2001.16.10.1765. J Bone Miner Res. 2001. PMID: 11585339
-
In vitro induction of neural differentiation of embryonic stem (ES) cells closely mimics molecular mechanisms of embryonic brain development.Pediatr Res. 2006 Apr;59(4 Pt 2):48R-53R. doi: 10.1203/01.pdr.0000203566.01600.8c. Pediatr Res. 2006. PMID: 16549548 Review.
-
The voyage of stem cell toward terminal differentiation: a brief overview.Acta Biochim Biophys Sin (Shanghai). 2012 Jun;44(6):463-75. doi: 10.1093/abbs/gms027. Epub 2012 May 3. Acta Biochim Biophys Sin (Shanghai). 2012. PMID: 22562866 Review.
Cited by
-
Enhanced yield of neuroepithelial precursors and midbrain-like dopaminergic neurons from human embryonic stem cells using the bone morphogenic protein antagonist noggin.Stem Cells. 2007 Feb;25(2):411-8. doi: 10.1634/stemcells.2006-0380. Epub 2006 Oct 12. Stem Cells. 2007. PMID: 17038668 Free PMC article.
-
Human embryonic stem cells: derivation, culture, and differentiation: a review.Restor Neurol Neurosci. 2010;28(4):589-603. doi: 10.3233/RNN-2010-0543. Restor Neurol Neurosci. 2010. PMID: 20714081 Free PMC article. Review.
-
Development of the hemangioblast defines the onset of hematopoiesis in human ES cell differentiation cultures.Blood. 2007 Apr 1;109(7):2679-87. doi: 10.1182/blood-2006-09-047704. Blood. 2007. PMID: 17148580 Free PMC article.
-
High-efficiency induction of neural conversion in human ESCs and human induced pluripotent stem cells with a single chemical inhibitor of transforming growth factor beta superfamily receptors.Stem Cells. 2010 Oct;28(10):1741-50. doi: 10.1002/stem.504. Stem Cells. 2010. PMID: 20734356 Free PMC article.
-
Mesenchymal Stem Cells for Spinal Cord Injury: Current Options, Limitations, and Future of Cell Therapy.Int J Mol Sci. 2019 May 31;20(11):2698. doi: 10.3390/ijms20112698. Int J Mol Sci. 2019. PMID: 31159345 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Miscellaneous
